{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:41:46Z","timestamp":1777930906347,"version":"3.51.4"},"reference-count":33,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T00:00:00Z","timestamp":1724371200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"University of California - Davis","award":["Phil and Karen C. Drayer Wildlife Health Center Fe"],"award-info":[{"award-number":["Phil and Karen C. Drayer Wildlife Health Center Fe"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:p>Rabbit hemorrhagic disease virus 2 (RHDV2; Caliciviridae, Lagovirus europaeus), the cause of a highly transmissible and fatal lagomorph disease, has spread rapidly through the western United States and Mexico, resulting in substantial mortality in domestic and wild rabbits. The disease was first detected in California in May 2020, prompting an interagency\/zoo\/academia\/nonprofit team to implement emergency conservation actions to protect endangered riparian brush rabbits ( Sylvilagus bachmani riparius) from RHDV2. Prior to vaccinating wild rabbits, we conducted a vaccine safety trial by giving a single SC dose of Filavac VHD K C+V (Filavie) vaccine to 19 adult wild riparian brush rabbits captured and temporarily held in captivity. Rabbits were monitored for adverse effects, and serum was collected before vaccination, and at 7\u201310, 14\u201320, and 60\u2009d post-vaccination. Sera were tested using an ELISA to determine antibody response and timing of seroconversion. Reverse-transcription quantitative real-time PCR (RT-qPCR) was performed on rectal swabs to evaluate infection status. No adverse effects from the vaccine were observed. Before vaccination, 18 of 19 rabbits were seronegative, and RHDV2 was not detected by RT-qPCR on any rectal swabs. After vaccination, all rabbits developed an antibody response, with titers of 1:10\u20131:160. Seroconversion generally occurred at 7\u201310\u2009d. The duration of antibody response was \u226560\u2009d in 12 of 13 rabbits. Sixteen animals were released and 4 were recaptured several months later, offering a glimpse into longer duration immune response. Our study has informed vaccination strategies for this species and serves as a model for protecting other vulnerable lagomorphs against RHDV2.<\/jats:p>","DOI":"10.1177\/10406387241267850","type":"journal-article","created":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T03:40:40Z","timestamp":1724384440000},"page":"735-744","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Antibody response of endangered riparian brush rabbits to vaccination against rabbit hemorrhagic disease virus 2"],"prefix":"10.1177","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0379-2499","authenticated-orcid":false,"given":"Megan E.","family":"Moriarty","sequence":"first","affiliation":[{"name":"Wildlife Health Laboratory, California Department of Fish and Wildlife, Rancho Cordova, CA, USA"},{"name":"Department of Medicine and Epidemiology, Karen C. Drayer Wildlife Health Center, School of Veterinary Medicine, University of California\u2013Davis, Davis, CA, USA"},{"name":"Current affiliations: University of California \u2013 Santa Cruz, Santa Cruz, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaime L.","family":"Rudd","sequence":"additional","affiliation":[{"name":"Wildlife Health Laboratory, California Department of Fish and Wildlife, Rancho Cordova, CA, USA"},{"name":"Endangered Species Recovery Program, Stanislaus State University, Turlock, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumika","family":"Takahashi","sequence":"additional","affiliation":[{"name":"San Luis National Wildlife Refuge Complex, U.S. Fish and Wildlife Service, Los Banos, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Hopson","sequence":"additional","affiliation":[{"name":"San Luis National Wildlife Refuge Complex, U.S. Fish and Wildlife Service, Los Banos, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colleen","family":"Kinzley","sequence":"additional","affiliation":[{"name":"Department of Animal Care, Conservation and Research, Oakland Zoo\u2013Conservation Society of California, Oakland, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darren","family":"Minier","sequence":"additional","affiliation":[{"name":"Department of Animal Care, Conservation and Research, Oakland Zoo\u2013Conservation Society of California, Oakland, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex","family":"Herman","sequence":"additional","affiliation":[{"name":"Department of Animal Care, Conservation and Research, Oakland Zoo\u2013Conservation Society of California, Oakland, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary Lou","family":"Berninger","sequence":"additional","affiliation":[{"name":"Foreign Animal Disease Diagnostic Laboratory, Plum Island Animal Disease Center, Greenport, NY, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fawzi","family":"Mohamed","sequence":"additional","affiliation":[{"name":"Foreign Animal Disease Diagnostic Laboratory, Plum Island Animal Disease Center, Greenport, NY, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muzafar","family":"Makhdoomi","sequence":"additional","affiliation":[{"name":"Foreign Animal Disease Diagnostic Laboratory, Plum Island Animal Disease Center, Greenport, NY, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leslie W.","family":"Woods","sequence":"additional","affiliation":[{"name":"California Animal Health and Food Safety Laboratory, University of California\u2013Davis, Davis, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hon S.","family":"Ip","sequence":"additional","affiliation":[{"name":"U.S. Geological Survey, National Wildlife Health Center, Madison, WI, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deana L.","family":"Clifford","sequence":"additional","affiliation":[{"name":"Wildlife Health Laboratory, California Department of Fish and Wildlife, Rancho Cordova, CA, USA"},{"name":"Department of Medicine and Epidemiology, Karen C. Drayer Wildlife Health Center, School of Veterinary Medicine, University of California\u2013Davis, Davis, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2024,8,23]]},"reference":[{"key":"bibr1-10406387241267850","doi-asserted-by":"publisher","DOI":"10.1186\/1297-9716-43-12"},{"key":"bibr2-10406387241267850","doi-asserted-by":"publisher","DOI":"10.1177\/10406387211006353"},{"key":"bibr3-10406387241267850","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.14315"},{"key":"bibr4-10406387241267850","doi-asserted-by":"publisher","DOI":"10.1155\/2023\/1869692"},{"key":"bibr5-10406387241267850","unstructured":"California Department of Fish and Wildlife. Five-year status review of riparian brush rabbit (\n                      Sylvilagus bachmani riparius\n                      ). Version 4. 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