{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T18:58:52Z","timestamp":1790449132583,"version":"4.1.0"},"reference-count":36,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2024,9,20]],"date-time":"2024-09-20T00:00:00Z","timestamp":1726790400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>Bacillary hemoglobinuria (BH) is an infectious disease, mostly affecting cattle, caused by Clostridium haemolyticum ( C. novyi type D), with acute hepatic necrosis and intravascular hemolysis. Cattle are typically predisposed to BH by liver injury caused by Fasciola hepatica, although cases have been reported in cattle without evidence of this parasite. Here we describe a cluster of 14 BH cases from 7 counties in north-central to central Missouri submitted to a veterinary diagnostic laboratory between December 2020 and April 2023. Postmortem examination in all cases revealed hemoglobinuria and acute hepatic necrosis with large numbers of gram-positive bacilli with terminal-to-subterminal spores. Flukes, fluke ova, and\/or fluke pigment consistent with Fascioloides magna were identified in 12 of 14 cases. Sequences of the nuclear ribosomal internal transcribed spacer 1 (ITS1) from one fluke had 100% identity to F. magna. C. novyi was detected by fluorescent antibody testing of liver impression smears (11 of 12 cases) and by immunohistochemistry of liver sections (7 of 7 cases). PCR on formalin-fixed, paraffin-embedded tissues amplified the C. haemolyticum beta toxin gene in each of the 7 cases tested. To our knowledge, a confirmed cluster of BH associated with F. magna has not been reported previously in cattle.<\/jats:p>","DOI":"10.1177\/10406387241280741","type":"journal-article","created":{"date-parts":[[2024,9,20]],"date-time":"2024-09-20T06:52:53Z","timestamp":1726815173000},"page":"126-134","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Bacillary hemoglobinuria in beef cattle infected with\n                    <i>Fascioloides magna<\/i>\n                    in Missouri"],"prefix":"10.1177","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3330-3759","authenticated-orcid":false,"given":"Rosalie A.","family":"Ierardi","sequence":"first","affiliation":[{"name":"Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, Columbia, MO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annabelle L.","family":"Burnum","sequence":"additional","affiliation":[{"name":"Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, Columbia, MO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren E.","family":"Camp","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology, and Immunology, University of California\u2013Davis, Davis, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren E.","family":"Delaney","sequence":"additional","affiliation":[{"name":"Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, Columbia, MO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Gull","sequence":"additional","affiliation":[{"name":"Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, Columbia, MO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brett M.","family":"Havis","sequence":"additional","affiliation":[{"name":"Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, Columbia, MO, USA"},{"name":"Idexx Laboratories, Westbrook, ME, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gayle C.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, Columbia, MO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3022-8888","authenticated-orcid":false,"given":"Dae Young","family":"Kim","sequence":"additional","affiliation":[{"name":"Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, Columbia, MO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6255-1121","authenticated-orcid":false,"given":"Kei","family":"Kuroki","sequence":"additional","affiliation":[{"name":"Veterinary Medical Diagnostic Laboratory, University of Missouri, Columbia, MO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1814-2392","authenticated-orcid":false,"given":"Renata M.","family":"Mammone","sequence":"additional","affiliation":[{"name":"Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, Columbia, MO, USA"},{"name":"Memorial Sloan Kettering Cancer Center, New York City, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William J.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, Columbia, MO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauricio A.","family":"Navarro","sequence":"additional","affiliation":[{"name":"California Animal Health and Food Safety Laboratory, San Bernardino, CA"},{"name":"Instituto de Patolog\u00eda Animal, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis A.","family":"Rivero","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia, MO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Shapiro","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology, and Immunology, University of California\u2013Davis, Davis, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0624-0789","authenticated-orcid":false,"given":"Amanda C.","family":"Smith","sequence":"additional","affiliation":[{"name":"Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, Columbia, MO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Courtney M.","family":"Valerio","sequence":"additional","affiliation":[{"name":"Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, Columbia, MO, USA"},{"name":"Department of Comparative, Diagnostic, and Population Medicine, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"III","given":"Fred","family":"Williams","sequence":"additional","affiliation":[{"name":"Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of 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