{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T23:44:17Z","timestamp":1790207057947,"version":"4.1.0"},"reference-count":45,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T00:00:00Z","timestamp":1734393600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Mark and Pat DuMont Equine Orthopedics Research Fund"},{"name":"Townsend Equine Health Research Fund"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    Prompt diagnosis of equine septic arthritis is crucial for successful treatment. Serum amyloid A (SAA) has been suggested as a reliable biomarker. However, we previously found that synovial fluid SAA increases in nonaffected joints of horses with septic arthritis. We hypothesized that systemic SAA may leak into the nonaffected joints. If this is the case, we also hypothesized that locally produced joint SAA isoforms may be better candidates for septic arthritis biomarkers. Thus, our objectives were 1) to evaluate the temporal kinetics of systemic and synovial fluid SAA in horses with septic arthritis ( n\u2009=\u20095), non-septic synovitis ( n\u2009=\u20095), and systemic inflammation ( n\u2009=\u20095), examining both affected and contralateral joints; and 2) investigate putative locally produced joint SAA isoforms and detect amino-acid differences between them. We confirmed that SAA increases significantly in synovial fluid in nonaffected joints of horses with systemic inflammation (\u2264352\u2009mg\/L), as well as in contralateral nonaffected joins in horses with septic arthritis (\u22641,830\u2009mg\/L) compared to baseline at time 0 (&lt;0.2\u2009mg\/L). We also identified a putative locally produced joint SAA peptide in synovial fluid (FGDSGHGAADSR) that differed in 1 amino acid from 2 systemic peptides found both in plasma and synovial fluid. The putative joint SAA isoform was present in joints of horses with both septic arthritis and systemic inflammation (ion intensities 10\n                    <jats:sup>4<\/jats:sup>\n                    \u201310\n                    <jats:sup>6<\/jats:sup>\n                    ). Thus, the increase of synovial fluid SAA may be both due to the leakage of SAA from serum into joints and local production of joint SAA isoforms.\n                  <\/jats:p>","DOI":"10.1177\/10406387241299873","type":"journal-article","created":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T07:18:34Z","timestamp":1734419914000},"page":"42-54","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Temporal kinetics of serum amyloid A (SAA) concentration and identification of SAA isoforms in blood and synovial fluid of horses with experimentally induced septic arthritis, non-septic synovitis, and systemic inflammation"],"prefix":"10.1177","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1009-7814","authenticated-orcid":false,"given":"Roman V.","family":"Koziy","sequence":"first","affiliation":[{"name":"Departments of Veterinary Pathology, University of Saskatchewan, Saskatoon, SK, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0869-7300","authenticated-orcid":false,"given":"George S.","family":"Katselis","sequence":"additional","affiliation":[{"name":"Western College of Veterinary Medicine, and Canadian Centre for Rural and Agricultural Health, Department of Medicine, College of Medicine, University of Saskatchewan, Saskatoon, SK, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seiji","family":"Yoshimura","sequence":"additional","affiliation":[{"name":"Large Animal Clinical Sciences, University of Saskatchewan, Saskatoon, SK, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elemir","family":"Simko","sequence":"additional","affiliation":[{"name":"Departments of Veterinary Pathology, University of Saskatchewan, Saskatoon, SK, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 L.","family":"Bracamonte","sequence":"additional","affiliation":[{"name":"Large Animal Clinical Sciences, University of Saskatchewan, Saskatoon, SK, 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