{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T14:05:27Z","timestamp":1780581927145,"version":"3.54.1"},"reference-count":41,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T00:00:00Z","timestamp":1737936000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:p>Phenobarbital is a common antiseizure medication that has a relatively narrow therapeutic window. Therapeutic drug monitoring (TDM) is a helpful tool to guide dose adjustments for phenobarbital and avoid its toxicity. We investigated the agreement among 3 methods of quantifying phenobarbital in canine plasma: high-performance liquid chromatography (HPLC), point-of-care (POC) testing, and the FDA-approved immunoassay analyzer. We randomly selected 45 plasma samples obtained by the TDM service (College of Veterinary Medicine, Auburn University, Auburn, AL, USA). Passing\u2013Bablok regression and Lin concordance correlation coefficients (CCCs) were used to determine the agreement of the results obtained for the 3 methods; Bland\u2013Altman plots were used for bias analysis using the results from the HPLC method as a reference. The FDA-approved immunoassay analyzer and POC immunoassay method results agreed with the HPLC. The results from the FDA-approved immunoassay analyzer were better correlated than those from the POC method, with Lin CCCs of 0.96 (95% CI: 0.93\u20130.98) and 0.94 (95% CI: 0.90\u20130.97), respectively. The average biases of the FDA-approved and the POC immunoassay analyzers were 0.80 and \u22120.64\u2009\u00b5g\/mL, respectively. Based on the CIs of Lin CCCs, the commercial POC phenobarbital test is a good screening tool and agrees with the HPLC method. However, the FDA-approved immunoassay analyzer method allows for more accurate quantification of phenobarbital concentrations, which is required for appropriate dose adjustment of phenobarbital.<\/jats:p>","DOI":"10.1177\/10406387241312891","type":"journal-article","created":{"date-parts":[[2025,1,29]],"date-time":"2025-01-29T13:19:13Z","timestamp":1738156753000},"page":"272-277","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparison of 3 methods of quantification of phenobarbital in canine plasma: high-performance liquid chromatography, a point-of-care immunoassay, and the FDA-approved immunoassay analyzer"],"prefix":"10.1177","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4971-0938","authenticated-orcid":false,"given":"Kamoltip","family":"Thungrat","sequence":"first","affiliation":[{"name":"Departments of Anatomy, Physiology, and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, AL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1314-7448","authenticated-orcid":false,"given":"Ryan","family":"Gibson","sequence":"additional","affiliation":[{"name":"Departments of Anatomy, Physiology, and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, AL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Jukier","sequence":"additional","affiliation":[{"name":"Clinical Sciences, College of Veterinary Medicine, Auburn University, Auburn, AL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3809-457X","authenticated-orcid":false,"given":"Alex","family":"Jenkins","sequence":"additional","affiliation":[{"name":"Departments of Anatomy, Physiology, and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, AL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Crisanta","family":"Cruz-Espindola","sequence":"additional","affiliation":[{"name":"Departments of Anatomy, Physiology, and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, AL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,1,27]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600770910"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.153.1.13"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tjem.2018.08.001"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2007.254"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/vrc2.608"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-9-202"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1179\/acb.2004.039"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-015-0464-z"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.11613\/BM.2011.010"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1177\/096228029900800204"},{"key":"e_1_3_2_12_2","first-page":"932","volume-title":"Small Animal Clinical Pharmacology and Therapeutics","author":"Boothe DM","year":"2011","unstructured":"Boothe DM. 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