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Here, we employed flow cytometry to document the specificity of mAbs submitted to the canine workshop and the animal homologue section of the human HLDA8 international workshop. We also provide an initial characterization of new mAbs derived from hybridomas developed from mice immunized with leukocytes from the blood of a healthy dog and from mice immunized with cells from a dog with B-cell leukemia. These mAbs enhance the tools available for characterizing leukemias, lymphomas, and other hematologic disorders in dogs, as well as for researching the canine immune response to pathogens. Importantly, some of the mAbs submitted to the canine and HLDA8 workshop recognize highly conserved epitopes expressed on orthologues of cluster of differentiation molecules first identified in humans and present an opportunity to develop a cross-species panel for clinical testing and research.<\/jats:p>","DOI":"10.1177\/10406387251315530","type":"journal-article","created":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T00:20:57Z","timestamp":1738801257000},"page":"298-304","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Update on development of monoclonal antibodies for use in clinical flow cytometry and research in dogs"],"prefix":"10.1177","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2062-6978","authenticated-orcid":false,"given":"Cleverson","family":"de Souza","sequence":"first","affiliation":[{"name":"Department of Comparative, Diagnostic and Population Medicine, University of Florida, Gainesville, FL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janean","family":"Fidel","sequence":"additional","affiliation":[{"name":"Veterinary Clinical Sciences, Washington State University, Pullman, WA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William C.","family":"Davis","sequence":"additional","affiliation":[{"name":"Departments of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,2,5]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00347-08"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0198-8859(84)90051-X"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1994.tb02315.x"},{"key":"e_1_3_2_5_2","first-page":"149","article-title":"Use of monoclonal antibodies and flow cytometry to cluster and analyze leukocyte differentiation molecules","volume":"45","author":"Davis WC","year":"1995","unstructured":"Davis WC, et al. Use of monoclonal antibodies and flow cytometry to cluster and analyze leukocyte differentiation molecules. Methods Mol Biol 1995;45:149\u2013167.","journal-title":"Methods Mol Biol"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2427(87)90005-5"},{"key":"e_1_3_2_7_2","first-page":"17","article-title":"Culture conditions that optimize outgrowth of hybridomas","volume":"45","author":"Hamilton MJ","year":"1995","unstructured":"Hamilton MJ, Davis WC. Culture conditions that optimize outgrowth of hybridomas. 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