{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:41:59Z","timestamp":1777930919882,"version":"3.51.4"},"reference-count":23,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2025,2,12]],"date-time":"2025-02-12T00:00:00Z","timestamp":1739318400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:p>\n                    Primary neoplastic diseases of the CNS in livestock are rare compared to companion animals. Here we describe the clinical, pathologic, and genetic findings in an adult sheep with a high-grade cerebral astrocytoma. Clinically, apathy, ataxia, bilateral amaurosis, and horizontal resting nystagmus were observed. The signs were suggestive of a space-occupying lesion affecting the vestibular and ascending reticular activating systems. The CSF had high total protein, hypercellularity, and pleocytosis. Macroscopically, a friable, gray-pink, ovoid mass (6\u2009\u00d7\u20093\u2009\u00d7\u20094\u2009cm) was observed within most of the right parietal, occipital, and temporal cerebral subcortical areas, extended into the thalamus, hippocampus, and the cerebral peduncles, and reached the ipsilateral choroid plexus and the lateral ventricles. Histologically, the well-delimited, unencapsulated lesion consisted of proliferating neoplastic spindloid-to-polygonal cells mainly arranged in streams and bundles supported by minimal fibrous stroma, and random areas of coagulative necrosis and hemorrhages without pseudopalisading in the center of the mass. Immunohistochemistry revealed strong immunolabeling of spindle cells for S100, vimentin, and glial fibrillary acidic protein. Immunolabeling for neuron-specific enolase was restricted to trapped neurons; oligodendrocyte transcription factor 2 (OLIG2), pan-cytokeratins, and SOX10 were immunonegative.\n                    <jats:italic>TP53<\/jats:italic>\n                    gene sequencing of the neoplasm did not demonstrate any point mutations involving exons 2\u201311.\n                  <\/jats:p>","DOI":"10.1177\/10406387251318694","type":"journal-article","created":{"date-parts":[[2025,2,12]],"date-time":"2025-02-12T23:48:41Z","timestamp":1739404121000},"page":"499-503","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["High-grade astrocytoma in a sheep: clinical, pathology, and genetic investigations"],"prefix":"10.1177","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8172-194X","authenticated-orcid":false,"given":"Sebastian A.","family":"Mignacca","sequence":"first","affiliation":[{"name":"Pathology and Animal Sciences Department, Animal and Plant Health Agency Weybridge, Addlestone, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timm","family":"Konold","sequence":"additional","affiliation":[{"name":"Pathology and Animal Sciences Department, Animal and Plant Health Agency Weybridge, Addlestone, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Gallisai","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sardegna, Laboratorio di Anatomo-Istopatologia e Genetica Animale, Sassari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caterina","family":"Maestrale","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sardegna, Laboratorio di Anatomo-Istopatologia e Genetica Animale, Sassari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Gomez-Vitores","sequence":"additional","affiliation":[{"name":"Pathology and Animal Sciences Department, Animal and Plant Health Agency Weybridge, Addlestone, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Nunez","sequence":"additional","affiliation":[{"name":"Pathology and Animal Sciences Department, Animal and Plant Health Agency Weybridge, Addlestone, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,2,12]]},"reference":[{"key":"e_1_3_3_2_2","unstructured":"Adjou K et al. Principali patologie del sistema nervoso degli ovini [Main pathologies of nervous system of ovine]. Summa 2007;3:11\u201319. Italian. https:\/\/www.summaanimalidareddito.it\/archivio\/?get=1"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/0300985818824952"},{"key":"e_1_3_3_4_2","unstructured":"Bernardini M. Esami collaterali\u2014Prelievo ed esami del liquor [Ancillary exams\u2014CSF sampling and testing]. In: Bernardini M ed. Neurologia del cane e del gatto [Dog and Cat Neurology]. 2nd ed. Poletto Editore 2010:85\u201394. Italian. https:\/\/distribuzione.evsrl.it\/ArticleDetail.aspx?lang=en&from=HP&id=4128"},{"key":"e_1_3_3_5_2","article-title":"TP53, ATRX alterations, and low tumor mutation load feature IDH-wildtype giant cell glioblastoma despite exceptional ultra-mutated tumors","volume":"2","author":"Cantero D","year":"2020","unstructured":"Cantero D, et al. TP53, ATRX alterations, and low tumor mutation load feature IDH-wildtype giant cell glioblastoma despite exceptional ultra-mutated tumors. Neurooncol Adv 2020;2:vdz059.","journal-title":"Neurooncol Adv"},{"key":"e_1_3_3_6_2","first-page":"250","volume-title":"Jubb, Kennedy, and Palmer\u2019s Pathology of Domestic Animals","author":"Cantile C","unstructured":"Cantile C, et al. Nervous system. In: Maxie MG, ed. Jubb, Kennedy, and Palmer\u2019s Pathology of Domestic Animals. 6th ed. Vol. 1. Elsevier, 2016:250\u2013406.","edition":"6"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1024\/0036-7281.147.10.453"},{"key":"e_1_3_3_8_2","first-page":"269","article-title":"Cerebral astrocytoma in a sheep","volume":"8","author":"Farjanikish G","year":"2017","unstructured":"Farjanikish G, et al. Cerebral astrocytoma in a sheep. 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