{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T20:30:24Z","timestamp":1793824224276,"version":"4.6.1"},"reference-count":22,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2025,2,24]],"date-time":"2025-02-24T00:00:00Z","timestamp":1740355200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100005825","name":"USDA National Institute of Food and Agriculture","doi-asserted-by":"crossref","award":["7004199"],"award-info":[{"award-number":["7004199"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Office of Research and Graduate studies","award":["RGS130532"],"award-info":[{"award-number":["RGS130532"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:p>\n                    The bovine respiratory disease complex (BRDC) is a multifactorial disease of economic importance in cattle involving viral and bacterial agents and several environmental and host-associated predisposing factors.\n                    <jats:italic>Mycoplasmopsis<\/jats:italic>\n                    (\n                    <jats:italic>Mycoplasma<\/jats:italic>\n                    )\n                    <jats:italic>bovis<\/jats:italic>\n                    is frequently detected in BRDC cases, but the role of this bacterium in the pathogenesis of BRDC is not completely understood. We explored the utility of routine histopathology and compared immunohistochemistry (IHC) and in situ hybridization (ISH) in pneumonic bovine lung tissue samples for the detection of\n                    <jats:italic>M. bovis<\/jats:italic>\n                    infection. Samples were analyzed for\n                    <jats:italic>M. bovis<\/jats:italic>\n                    using mycoplasma bacterial culture (screening test), H&amp;E staining, IHC, and ISH. We found that \u201ccompatible histologic lesions\u201d are not entirely predictive of the presence of\n                    <jats:italic>M. bovis<\/jats:italic>\n                    on culture, IHC, or ISH, and also that there was no statistical difference between IHC and ISH for detecting\n                    <jats:italic>M. bovis<\/jats:italic>\n                    . We conclude that IHC and ISH can be used interchangeably to detect\n                    <jats:italic>M. bovis<\/jats:italic>\n                    infections in pneumonic bovine lung.\n                  <\/jats:p>","DOI":"10.1177\/10406387251322463","type":"journal-article","created":{"date-parts":[[2025,2,24]],"date-time":"2025-02-24T23:42:07Z","timestamp":1740440527000},"page":"448-454","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Detection of\n                    <i>Mycoplasmopsis<\/i>\n                    (\n                    <i>Mycoplasma<\/i>\n                    )\n                    <i>bovis<\/i>\n                    in formalin-fixed, paraffin-embedded bovine lung sections by immunohistochemistry and in situ hybridization"],"prefix":"10.1177","volume":"37","author":[{"given":"Matthew","family":"Brown","sequence":"first","affiliation":[{"name":"Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zackary","family":"Laney","sequence":"additional","affiliation":[{"name":"Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laila","family":"Tabatabai","sequence":"additional","affiliation":[{"name":"Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Hassebroek","sequence":"additional","affiliation":[{"name":"Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Lahmers","sequence":"additional","affiliation":[{"name":"Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tessa","family":"LeCuyer","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, University of California\u2013Davis, San Bernardino, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0681-1878","authenticated-orcid":false,"given":"Francisco A.","family":"Uzal","sequence":"additional","affiliation":[{"name":"California Animal Health and Food Safety Laboratory, San Bernardino branch, University of California\u2013Davis, San Bernardino, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5115-9949","authenticated-orcid":false,"given":"Francisco R.","family":"Carvallo","sequence":"additional","affiliation":[{"name":"Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,2,24]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063879500700217"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063879500700306"},{"key":"e_1_3_3_4_2","volume-title":"Proc ACVP\/ASVCP Annual Meeting","author":"Carvallo","year":"2023","unstructured":"Carvallo, et al Real time PCR for the detection of Mycoplasma bovis and viral pathogens of calf pneumonia in southwest Virginia based on formalin-fixed paraffin embedded specimens. In: Proc ACVP\/ASVCP Annual Meeting; Chicago, IL; Oct 2023."},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvfa.2010.03.003"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-7020-5318-4.00011-5"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvfa.2010.04.004"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.12597"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/1751-0147-54-9"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1177\/1040638711435145"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep15557"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0442.2004.00596.x"},{"key":"e_1_3_3_13_2","first-page":"547","volume-title":"Pathologic Basis of Veterinary Disease","author":"L\u00f3pez A","year":"2022","unstructured":"L\u00f3pez A, Martinson SA. Respiratory system, thoracic cavities, mediastinum and pleurae. In: Zachary JF, ed. Pathologic Basis of Veterinary Disease. 7th ed. Elsevier, 2022:547\u2013642.","edition":"7"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvfa.2008.10.011"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2011.0750.x"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46058-0"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5288(02)00155-8"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.15135"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvfa.2020.02.002"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12907-018-0074-3"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.4103\/picr.PICR_123_17"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9975(96)80037-5"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2018.06.007"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10406387251322463","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/10406387251322463","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10406387251322463","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:28:29Z","timestamp":1777681709000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/10406387251322463"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,24]]},"references-count":22,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1177\/10406387251322463"],"URL":"https:\/\/doi.org\/10.1177\/10406387251322463","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,24]]}}}