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Advancements include the development of a direct detection method for\n                    <jats:italic>T. foetus<\/jats:italic>\n                    using reverse-transcription quantitative real-time PCR. We compared the stability of samples in 3 different media: PBS, sterile saline, and lactated Ringer solution over periods of 24\u2013120\u2009h, with storage temperatures of 0\u00b0C, 20\u00b0C, 37\u00b0C, and 54.5\u00b0C to mimic shipping of samples in various environmental temperatures.\n                  <\/jats:p>","DOI":"10.1177\/10406387251330297","type":"journal-article","created":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T08:40:41Z","timestamp":1744792841000},"page":"592-596","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluating the stability of\n                    <i>Tritrichomonas foetus<\/i>\n                    in various media under simulated environmental conditions of shipment for RT-qPCR assays"],"prefix":"10.1177","volume":"37","author":[{"given":"SaraBeth","family":"Boggan","sequence":"first","affiliation":[{"name":"Texas Tech School of Veterinary Medicine, Amarillo, TX, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5718-753X","authenticated-orcid":false,"given":"Ryan B.","family":"Williams","sequence":"additional","affiliation":[{"name":"Texas Tech School of Veterinary Medicine, Amarillo, TX, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0096-5040","authenticated-orcid":false,"given":"Jennifer H.","family":"Koziol","sequence":"additional","affiliation":[{"name":"Texas Tech School of Veterinary Medicine, Amarillo, TX, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,4,16]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0749-0720(15)30346-7"},{"key":"e_1_3_3_3_2","first-page":"111","volume-title":"Parasitic Protozoa","author":"BonDurant RH","unstructured":"BonDurant RH, Honigberg BM. 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