{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:42:16Z","timestamp":1777930936474,"version":"3.51.4"},"reference-count":24,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T00:00:00Z","timestamp":1748908800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T00:00:00Z","timestamp":1748908800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100014612","name":"parks canada","doi-asserted-by":"publisher","award":["1-426636-1140-71001-8000-27701"],"award-info":[{"award-number":["1-426636-1140-71001-8000-27701"]}],"id":[{"id":"10.13039\/100014612","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:p>\n                    Bovine tuberculosis, caused by\n                    <jats:italic>Mycobacterium bovis<\/jats:italic>\n                    , is endemic in the Wood Buffalo National Park, Canada, home to free-ranging and threatened wood bison. This disease poses a threat to the conservation of this culturally and ecologically important animal species, as well as potentially impacting the health of humans and other animal species via zoonosis and spillover, respectively. The ability to detect infection early will minimize and prevent the potential risk of\n                    <jats:italic>M. bovis<\/jats:italic>\n                    transmission. Interferon-gamma (IFN\u03b3) assays are a reliable detection method for\n                    <jats:italic>M. bovis<\/jats:italic>\n                    in cattle and other wildlife species and may have diagnostic value in bison as well. We aimed to optimize and partially validate 2 commercial IFN\u03b3 ELISAs to detect endogenous bison IFN\u03b3 in mitogen-stimulated whole blood. Parameters evaluated included antibody identification, sample matrix effect, dilution linearity, assay reproducibility, and limit of quantification. The optimized assays demonstrated linear responses to recombinant bovine and endogenous bison IFN\u03b3 (range: 1\u2013125\u2009pg\/mL;\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u2009=\u20090.99), with good recovery and fair reproducibility, and a low limit of quantification of 1\u2009pg\/mL. Mabtech bovine Flex and Pro kits have the same antibodies but in 2 different assay formats; an in-house assay platform (Flex kit) and precoated plates (Pro kit) are considered suitable for measuring bison IFN\u03b3, offering flexibility depending on available resources.\n                  <\/jats:p>","DOI":"10.1177\/10406387251344567","type":"journal-article","created":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T09:34:32Z","timestamp":1748943272000},"page":"764-770","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Optimization and validation of ELISAs for interferon-gamma determination in bison"],"prefix":"10.1177","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7825-6485","authenticated-orcid":false,"given":"Josephine","family":"Chileshe","sequence":"first","affiliation":[{"name":"Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, Saskatoon, Saskatchewan, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Todd","family":"Shury","sequence":"additional","affiliation":[{"name":"Parks Canada Agency, Government of Canada, Saskatoon, Saskatchewan, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey M.","family":"Chen","sequence":"additional","affiliation":[{"name":"Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, Saskatoon, Saskatchewan, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,6,3]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2015.00179"},{"key":"e_1_3_3_3_2","article-title":"Limit of blank, limit of detection and limit of quantification","volume":"29","author":"Armbruster DA","year":"2008","unstructured":"Armbruster DA, Pry T. Limit of blank, limit of detection and limit of quantification. Clin Biochem Rev 2008;29:S49\u2013S52.","journal-title":"Clin Biochem Rev"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006122"},{"key":"e_1_3_3_5_2","unstructured":"Canadian Food Inspection Agency (CFIA). Chapter 3.1. Tuberculosis testing. Disease Control Manual of Procedures 2017. https:\/\/inspection.canada.ca\/en\/animal-health\/terrestrial-animals\/diseases\/accredited-veterinarians-manual\/chapter-3#c1"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1177\/1040638719843955"},{"key":"e_1_3_3_7_2","unstructured":"Clinical and Laboratory Standards Institute (CLSI). Evaluation of detection capability for clinical laboratory measurement procedures; approved guideline. 2nd ed. CLSI 2012. CLSI document EP17-A2."},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5452.443"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens11020260"},{"key":"e_1_3_3_10_2","unstructured":"Environment and Climate Change Canada. Recovery strategy for the wood bison (Bison bison athabascae) in Canada. Species at Risk Act Recovery Strategy Series. Environment and Climate Change Canada 2018. https:\/\/www.canada.ca\/en\/environment-climate-change\/services\/species-risk-public-registry\/recovery-strategies\/wood-bison-2018.html"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2021.682466"},{"key":"e_1_3_3_12_2","first-page":"1","volume-title":"ELISA Technical Bulletin \u2013 No 3. Corning","author":"Gibbs J.","unstructured":"Gibbs J. Effective blocking procedures. ELISA Technical Bulletin \u2013 No 3. Corning, Life Sciences, 2001:1\u20136."},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.7589\/2018-03-089"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-46.1.78"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-023-06083-7"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2005.11.013"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00034-13"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.4103\/2229-5186.79345"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2020.110163"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1258\/acb.2011.011073"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-020-00881-y"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0087145"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2011.03.033"},{"key":"e_1_3_3_24_2","unstructured":"World Organization for Animal Health. Chapter 2.2.7. Principles and Methods for the Validation of Diagnostic Tests for Infectious Diseases Applicable to Wildlife. In: Terrestrial Manual 2023. https:\/\/www.oie.int\/fileadmin\/Home\/eng\/Health_standards\/tahm\/2.02.07_WILDLIFE.pdf"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2012.06.009"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10406387251344567","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/10406387251344567","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10406387251344567","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:29:05Z","timestamp":1777681745000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/10406387251344567"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,3]]},"references-count":24,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1177\/10406387251344567"],"URL":"https:\/\/doi.org\/10.1177\/10406387251344567","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,3]]}}}