{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T04:27:51Z","timestamp":1792988871069,"version":"4.5.0"},"reference-count":17,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T00:00:00Z","timestamp":1756080000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T00:00:00Z","timestamp":1756080000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100021039","name":"School of Veterinary Medicine, Ross University","doi-asserted-by":"publisher","award":["42015-2021"],"award-info":[{"award-number":["42015-2021"]}],"id":[{"id":"10.13039\/100021039","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:p>To date, the minimum centrifugation times of microhematocrit tubes of blood to generate accurate PCV and total solids (TS) results have not been validated in veterinary medicine. We collected blood samples from 44 donkeys, 43 dogs, 61 sheep, and 40 cattle. We centrifuged microhematocrit tubes for 1, 2, 3, 5, and 15\u2009min in donkeys and dogs, and 1.5, 3, 5, 10, and 15\u2009min in ruminants. We evaluated the agreements between PCV and TS values at each time of centrifugation with the reference values at 15\u2009min using intra-class coefficients of correlation and linear regressions. Finally, we considered the symmetrical distribution of differences between results obtained at each time of centrifugation and the reference values. We found that microhematocrit tubes centrifuged for a minimum of 3\u2009min in donkeys and dogs, 10\u2009min in sheep, and 5\u2009min in cattle gave PCV and TS results in agreement with the values obtained after 15\u2009min of centrifugation. The centrifugation time for cattle was shorter than currently advised. However, because PCV values of all cattle and most donkeys enrolled were within RIs and because no polycythemic animals were included, validation of these times may be warranted.<\/jats:p>","DOI":"10.1177\/10406387251362461","type":"journal-article","created":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T11:03:07Z","timestamp":1756119787000},"page":"873-881","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Estimation of minimum centrifugation time of microhematocrit tubes to obtain accurate results of packed cell volume and total solids in donkeys, dogs, sheep, and cows"],"prefix":"10.1177","volume":"37","author":[{"given":"Dreyton","family":"Oliva","sequence":"first","affiliation":[{"name":"Ross University School of Veterinary Medicine, Basseterre, Saint Kitts and Nevis"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8742-8303","authenticated-orcid":false,"given":"H\u00e9l\u00e8ne","family":"Lard\u00e9","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biomedical Sciences, Faculty of Veterinary Medicine, Universit\u00e9 de Montr\u00e9al, Campus de Rimouski, Rimouski, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2926-4926","authenticated-orcid":false,"given":"Juliette","family":"Bouillon","sequence":"additional","affiliation":[{"name":"Institut Polytechnique UniLaSalle, Universit\u00e9 d\u2019Artois, Mont-Saint-Aignan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4980-888X","authenticated-orcid":false,"given":"Ronan","family":"Whiston","sequence":"additional","affiliation":[{"name":"Departments of Biomedical Sciences, Basseterre, Saint Kitts and Nevis"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Peda","sequence":"additional","affiliation":[{"name":"Clinical Sciences, Basseterre, Saint Kitts and Nevis"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4076-094X","authenticated-orcid":false,"given":"Patricia","family":"Dowling","sequence":"additional","affiliation":[{"name":"Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3039-7710","authenticated-orcid":false,"given":"Ronan J. J.","family":"Chapuis","sequence":"additional","affiliation":[{"name":"Institut Polytechnique UniLaSalle, Universit\u00e9 d\u2019Artois, Mont-Saint-Aignan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,8,25]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12810"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/jsap.12603"},{"key":"e_1_3_3_4_2","unstructured":"Clinical and Laboratory Standards Institute (CLSI). Procedure for determining packed cell volume by the microhematocrit method. 3rd ed. CLSI 2000. CLSI document H07-A3."},{"key":"e_1_3_3_5_2","unstructured":"Cornell University College of Veterinary Medicine. eClinPath. Hematocrit\/packed cell volume. 2025. https:\/\/eclinpath.com\/hematology\/tests\/hematocrit\/"},{"key":"e_1_3_3_6_2","unstructured":"Fielder SE. Merck Manual. Hematology reference ranges. Reviewed\/revised Sept 2022 modified Sept 2024. https:\/\/www.merckvetmanual.com\/reference-values-and-conversion-tables\/reference-guides\/hematology-reference-ranges"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.11613\/BM.2015.015"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12101"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2006.tb00131.x"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcm.2016.02.012"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2021.103858"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12583"},{"key":"e_1_3_3_13_2","first-page":"386","article-title":"Erythrocyte sedimentation rate studies in sheep, dog, and horse","volume":"61","author":"Osbaldiston G.","year":"1971","unstructured":"Osbaldiston G. Erythrocyte sedimentation rate studies in sheep, dog, and horse. 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J S Afr Vet Assoc 2022;93:176\u2013178.","journal-title":"J S Afr Vet Assoc"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.01.003"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2017.00731"},{"key":"e_1_3_3_18_2","unstructured":"World Health Organization (WHO) Expert Panel on Cytometry of the International Council for Standardization in Haematology. Recommended method for the determination of packed cell volume by centrifugation. WHO Diagnostic Imaging and Laboratory Technology 2000."}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10406387251362461","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/10406387251362461","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10406387251362461","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:29:08Z","timestamp":1777681748000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/10406387251362461"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,25]]},"references-count":17,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1177\/10406387251362461"],"URL":"https:\/\/doi.org\/10.1177\/10406387251362461","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,25]]}}}