{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T21:02:14Z","timestamp":1786741334914,"version":"build-2736575974"},"reference-count":56,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T00:00:00Z","timestamp":1759449600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Rudolf Steiner Fund for Scientific Research e.V., Nuremberg, Germany"},{"name":"Stiftung Pro Pferd, Zurich, Switzerland"},{"name":"The Veterinary Association of the Canton of Neuch\u00e2tel, Neuch\u00e2tel, Switzerland"},{"name":"The Swiss Veterinary Association for Alternative and Complementary Medicine (camvet.ch), Bern, Switzerland"},{"name":"Society of Cancer Research, Arlesheim, Switzerland"},{"name":"Iscador AG, Arlesheim, Switzerland"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    We investigated microRNAs (miRNAs) as potential prognostic biomarkers for equine sarcoid (ES) disease. In a breed-, age-, and sex-matched case-controlled study involving 45 ES-affected and 15 control horses, we assessed the diagnostic, prognostic, and theragnostic value of 3 miRNAs (eca-miR-127, eca-miR-379, eca-miR-432) in horses treated with European mistletoe (\n                    <jats:italic toggle=\"yes\">Viscum album<\/jats:italic>\n                    ) extract versus placebo. Whole-blood miRNA concentrations were measured using reverse-transcription quantitative real-time PCR (RT-qPCR) at 3 different times. We found that eca-miR-432 expression was lower in ES-affected (median\u2009=\u2009\u22121.93; 95% CI: \u22122.03 to \u2212.86) compared to control (median\u2009=\u2009\u22121.71; 95% CI: \u22121.92 to \u22121.6) horses (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009=\u20090.03,\n                    <jats:italic toggle=\"yes\">r<\/jats:italic>\n                    \u2009=\u20090.3; 95% CI: 0.024\u20130.57) with a median difference of \u22121.93 versus \u22121.71, respectively. The ROC curve analysis indicated an area under the curve of 0.71 (95% CI: 0.51\u20130.84;\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009=\u20090.005) with a sensitivity of 74% (95% CI: 61\u201388%) and a specificity of 73% (95% CI: 39\u201394%) to diagnose ES. However, none of the miRNAs evaluated had prognostic potential or significant changes in expression following treatment. Additionally, miRNA expression was not influenced by breed, sex, or season. Although whole-blood eca-miR-432 had moderate diagnostic potential for ES, identifying prognostic miRNA biomarkers for ES remains a challenge.\n                  <\/jats:p>","DOI":"10.1177\/10406387251362820","type":"journal-article","created":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T07:12:27Z","timestamp":1759475547000},"page":"33-40","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Selected microRNAs as biomarkers in sarcoid-affected horses under immunotherapy with a mistletoe extract"],"prefix":"10.1177","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-4943-5151","authenticated-orcid":false,"given":"Anke","family":"Beermann","sequence":"first","affiliation":[{"name":"Departments of Livestock Sciences, Soil Sciences and Crop Sciences (FiBL), Research Institute of Organic Agriculture, Frick, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9798-842X","authenticated-orcid":false,"given":"Eman","family":"Hamza","sequence":"additional","affiliation":[{"name":"Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-6996-3022","authenticated-orcid":false,"given":"Sonja","family":"Reinhard","sequence":"additional","affiliation":[{"name":"Departments of Livestock Sciences, Soil Sciences and Crop Sciences (FiBL), Research Institute of Organic Agriculture, Frick, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4574-6952","authenticated-orcid":false,"given":"Christoph","family":"Koch","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, Swiss Institute of Equine Medicine (ISME), Vetsuisse Faculty, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9404-5271","authenticated-orcid":false,"given":"Thomas","family":"Oberh\u00e4nsli","sequence":"additional","affiliation":[{"name":"Departments of Livestock Sciences, Soil Sciences and Crop Sciences (FiBL), Research Institute of Organic Agriculture, Frick, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8238-8907","authenticated-orcid":false,"given":"Lucia","family":"Unger","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, Swiss Institute of Equine Medicine (ISME), Vetsuisse Faculty, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,10,3]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.2010.00267.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.17052"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.2016.1204483"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/vco.12458"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-12-3302"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.112797"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/evj.14434"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.3259"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.18947-0"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.00544"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2013.09.053"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2010.0597.x"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0261076"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gpb.2018.03.004"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20246285"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-7-14"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep39812"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2022.105916"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/vde.13236"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-022-09930-7"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2018.08.010"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.4161\/rna.8.4.16029"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.ctep.2005.10.008"},{"key":"e_1_3_3_25_2","article-title":"Pascoe\u2019s principles and practice of equine dermatology","author":"Knottenbelt DC.","year":"2009","unstructured":"Knottenbelt DC. Pascoe\u2019s principles and practice of equine dermatology. Elsevier Health Sci, 2009.","journal-title":"Elsevier Health Sci"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2018.08.009"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr1259"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.4097\/kjae.2016.69.6.555"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2019.1612217"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00371-13"},{"key":"e_1_3_3_31_2","unstructured":"Mangla P. Anomaly detection: how to find outliers using the Grubbs test. PyImageSearch. 2025 Jan 6. https:\/\/pyimagesearch.com\/2025\/01\/06\/anomaly-detection-how-to-find-outliers-using-the-grubbs-test\/ 2025."},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.140024"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1053\/tvjl.2000.0524"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/arch.21657"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-29514-3"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2018.00402"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-016-3168-2"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2017.02.017"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1159\/000488456"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2018.1551693"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.2460\/javma.242.1.99"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.30120"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0188085"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1111\/vco.12216"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.4168\/aair.2021.13.4.576"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.16027"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1111\/vco.12451"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.15375"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063870601800121"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2019.03.008"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.225391.117"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2014.10.064"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0187776"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules23040919"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-11-44"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.3390\/genes14091688"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2020.11.012"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10406387251362820","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/10406387251362820","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10406387251362820","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:29:09Z","timestamp":1777681749000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/10406387251362820"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,3]]},"references-count":56,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1177\/10406387251362820"],"URL":"https:\/\/doi.org\/10.1177\/10406387251362820","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,3]]}}}