{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:42:22Z","timestamp":1777930942619,"version":"3.51.4"},"reference-count":24,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T00:00:00Z","timestamp":1759881600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Thammasat University Research Fund","award":["TUFT 53\/2566"],"award-info":[{"award-number":["TUFT 53\/2566"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    The ability to accurately and rapidly identify causative agents of infectious diseases facilitates precise treatment, improves clinical outcomes, and augments epidemiology studies. For many veterinary and zoonotic pathogens, however, simple molecular tests for species identification are not available.\n                    <jats:italic toggle=\"yes\">Actinobacillus equuli<\/jats:italic>\n                    causes severe diseases, such as sleepy foal disease, septicemia, and meningitis in horses and pigs.\n                    <jats:italic toggle=\"yes\">A. equuli<\/jats:italic>\n                    can also cause severe diseases in humans bitten by infected animals. Existing\n                    <jats:italic toggle=\"yes\">A. equuli<\/jats:italic>\n                    identification methods are biochemical tests, 16S rRNA gene amplification followed by DNA sequencing, and MALDI-TOF MS. Nonetheless, differentiating among\n                    <jats:italic toggle=\"yes\">Actinobacillus<\/jats:italic>\n                    spp. by these methods is still challenging. We identified novel DNA markers specific to\n                    <jats:italic toggle=\"yes\">A. equuli<\/jats:italic>\n                    by computational genome analysis. We then designed PCR primers specific to\n                    <jats:italic toggle=\"yes\">A. equuli<\/jats:italic>\n                    based on\n                    <jats:italic toggle=\"yes\">A. equuli<\/jats:italic>\n                    marker sequences. We validated 2\n                    <jats:italic toggle=\"yes\">A. equuli<\/jats:italic>\n                    \u2013specific PCR assays using genomic DNA from 10 strains of\n                    <jats:italic toggle=\"yes\">A. equuli<\/jats:italic>\n                    , 15 strains of other\n                    <jats:italic toggle=\"yes\">Actinobacillus<\/jats:italic>\n                    species, and 5 other bacterial species. Both assays gave the PCR products of expected sizes for genomic DNA of all 10 strains of\n                    <jats:italic toggle=\"yes\">A. equuli<\/jats:italic>\n                    but not for those of other\n                    <jats:italic toggle=\"yes\">Actinobacillus<\/jats:italic>\n                    and other bacterial species. Our novel PCR assays can accelerate\n                    <jats:italic toggle=\"yes\">A. equuli<\/jats:italic>\n                    identification and disease diagnosis, leading to timely and appropriate antimicrobial treatment, and enable high-resolution epidemiologic studies.\n                  <\/jats:p>","DOI":"10.1177\/10406387251382186","type":"journal-article","created":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T05:23:21Z","timestamp":1759987401000},"page":"59-65","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["In silico\u2013derived\n                    <i>Actinobacillus equuli<\/i>\n                    \u2013specific DNA markers and development of associated PCR assays"],"prefix":"10.1177","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7824-5153","authenticated-orcid":false,"given":"Warangkhana","family":"Songsungthong","sequence":"first","affiliation":[{"name":"National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Sirindhorn International Institute of Technology, Thammasat University, Pathum Thani, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wichai","family":"Pornthanakasem","sequence":"additional","affiliation":[{"name":"National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Sirindhorn International Institute of Technology, Thammasat University, Pathum Thani, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ubolsree","family":"Leartsakulpanich","sequence":"additional","affiliation":[{"name":"National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Sirindhorn International Institute of Technology, Thammasat University, Pathum Thani, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6791-4411","authenticated-orcid":false,"given":"Gun","family":"Srijuntongsiri","sequence":"additional","affiliation":[{"name":"School of Information, Computer, and Communication Technology (ICT), Sirindhorn International Institute of Technology, Thammasat University, Pathum Thani, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,10,8]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2002.tb11145.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00048-2"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/9781118960608.gbm01197.pub2"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2023.1235837"},{"key":"e_1_3_2_6_2","first-page":"656","volume-title":"Equine Emergencies","author":"Borges AS","unstructured":"Borges AS, et al. Emergency diseases unique to countries outside the continental United States. In: Orsini JA, Divers TJ, eds. Equine Emergencies. 4th ed. Saunders, 2013:656\u2013686.","edition":"4"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2003.12.002"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-52-5-1569"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2015.07.097"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(22)00648-X"},{"key":"e_1_3_2_11_2","first-page":"1290","article-title":"Valvular endocarditis in the horse: 20 cases (1993\u20132020)","volume":"61","author":"Henderson B","year":"2020","unstructured":"Henderson B, et al. Valvular endocarditis in the horse: 20 cases (1993\u20132020). Can Vet J 2020;61:1290\u20131294.","journal-title":"Can Vet J"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens12020224"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1177\/1040638711416854"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00339-15"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2009.06.029"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.02785-0"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv681"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1053\/tvjl.1999.0403"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1032"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(00)00345-X"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-1036-7_1"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01311-21"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-023-01160-2"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/0471142727.mb0204s56"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-13-134"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10406387251382186","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/10406387251382186","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10406387251382186","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:29:18Z","timestamp":1777681758000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/10406387251382186"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,8]]},"references-count":24,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1177\/10406387251382186"],"URL":"https:\/\/doi.org\/10.1177\/10406387251382186","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,8]]}}}