{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:42:25Z","timestamp":1777930945430,"version":"3.51.4"},"reference-count":13,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T00:00:00Z","timestamp":1762128000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100032517","name":"IDEXX Laboratories","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100032517","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2026,1]]},"abstract":"\n Cystoisospora<\/jats:italic>\n species that infect dogs and cats are ubiquitous, and oocysts can be found in the feces of both subclinically infected and sick animals. Most clinical cases are diagnosed in puppies and kittens <4-mo-old. We validated a high-throughput immunoassay (Fecal Dx immunoassay; Idexx), which uses 2 monoclonal antibodies to detect\n Cystoisospora<\/jats:italic>\n spp. coproantigen, using zinc sulfate centrifugal flotation (ZCF) as the reference method and samples from experimentally infected animals. This new genus-level coproantigen assay had equivalent sensitivity for at least 4 relevant\n Cystoisospora<\/jats:italic>\n spp.\u2014\n C. canis<\/jats:italic>\n ,\n C. ohioensis\u2013<\/jats:italic>\n like,\n C. felis<\/jats:italic>\n , and\n C. rivolta<\/jats:italic>\n \u2014with no observed cross-reactivity to other protozoal parasites (\n Giardia<\/jats:italic>\n ,\n Cryptosporidium<\/jats:italic>\n ,\n Tritrichomonas<\/jats:italic>\n ,\n Eimeria<\/jats:italic>\n ) of companion animals. In comparison to ZCF, the immunoassay had a positive percent agreement of 88.5% (95% CI [86.1, 91.6]) and a negative percent agreement of 98.2% (95% CI [98.1, 98.2]). The prevalence of\n Cystoisospora<\/jats:italic>\n spp. detected by ZCF was 1%; the prevalence identified through coproantigen testing was 2.7% (95% CI [2.6, 2.8]). Among puppies and kittens <1-y-old, the prevalence according to coproantigen testing was 7.4% (95% CI [6.6, 8.2]) for puppies and 8.2% (95% CI [7.7, 8.6%]) for kittens; the prevalence detected by ZCF was 3.6% (95% CI [3.0, 3.9]) for puppies and 2.9% (95% CI [2.4, 3.4]) for kittens. Our results validate our\n Cystoisospora<\/jats:italic>\n coproantigen immunoassay as specific, precise, and sensitive.\n <\/jats:p>","DOI":"10.1177\/10406387251385940","type":"journal-article","created":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T07:23:00Z","timestamp":1762240980000},"page":"130-134","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Analytical validation of an immunoassay for the detection of\n Cystoisospora<\/i>\n spp. coproantigen in canine and feline fecal samples"],"prefix":"10.1177","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-9317-0275","authenticated-orcid":false,"given":"Amy L.","family":"Porter","sequence":"first","affiliation":[{"name":"IDEXX Laboratories, Westbrook, ME, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9081-7847","authenticated-orcid":false,"given":"Rita","family":"Hanna","sequence":"additional","affiliation":[{"name":"IDEXX Laboratories, Westbrook, ME, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-2545-8079","authenticated-orcid":false,"given":"Jill","family":"Saucier","sequence":"additional","affiliation":[{"name":"IDEXX Laboratories, Westbrook, ME, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3738-3115","authenticated-orcid":false,"given":"Haley","family":"Florio","sequence":"additional","affiliation":[{"name":"IDEXX Laboratories, Westbrook, ME, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-1615-2733","authenticated-orcid":false,"given":"Quinn","family":"Ferguson","sequence":"additional","affiliation":[{"name":"IDEXX Laboratories, Westbrook, ME, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3791-1665","authenticated-orcid":false,"given":"Todd","family":"Bezold","sequence":"additional","affiliation":[{"name":"IDEXX Laboratories, Westbrook, ME, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9057-4406","authenticated-orcid":false,"given":"Anne","family":"Westra","sequence":"additional","affiliation":[{"name":"IDEXX Laboratories, Westbrook, ME, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7969-3272","authenticated-orcid":false,"given":"Phillip","family":"Smith","sequence":"additional","affiliation":[{"name":"IDEXX Laboratories, Westbrook, ME, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6897-4169","authenticated-orcid":false,"given":"Tori","family":"Denis","sequence":"additional","affiliation":[{"name":"IDEXX Laboratories, Westbrook, ME, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8733-6014","authenticated-orcid":false,"given":"Jesse","family":"Buch","sequence":"additional","affiliation":[{"name":"IDEXX Laboratories, Westbrook, ME, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,11,3]]},"reference":[{"key":"e_1_3_3_2_2","article-title":"Limit of blank, limit of detection, and limit of quantitation","volume":"29","author":"Armbruster DA","year":"2008","unstructured":"Armbruster DA, Pry T. Limit of blank, limit of detection, and limit of quantitation. Clin Biochem Rev 2008;29(Suppl 1):S49\u2013S52.","journal-title":"Clin Biochem Rev"},{"key":"e_1_3_3_3_2","first-page":"483","article-title":"Prevalence of canine parasites based on fecal flotation","volume":"18","author":"Blagburn BL","year":"1996","unstructured":"Blagburn BL, et al. Prevalence of canine parasites based on fecal flotation. Compend Contin Educ Pract Vet 1996;18:483\u2013509.","journal-title":"Compend Contin Educ Pract Vet"},{"key":"e_1_3_3_4_2","first-page":"349","volume-title":"Georgis\u2019 Parasitology for Veterinarians","author":"Bowman DD.","year":"2020","unstructured":"Bowman DD. Diagnostic parasitology. In: Georgis\u2019 Parasitology for Veterinarians. 11th ed. Elsevier, 2020:349\u2013454.","edition":"11"},{"key":"e_1_3_3_5_2","unstructured":"Clinical Laboratory Standards Institute (CLSI). Evaluation of Detection Capability for Clinical Laboratory Measurement Procedures; Approved Guideline. 2nd ed. Guideline EP17-A2. CLSI 2012."},{"key":"e_1_3_3_6_2","first-page":"15","article-title":"Comparison of common fecal flotation techniques for the recovery of parasite eggs and oocysts","volume":"6","author":"Dryden MW","year":"2005","unstructured":"Dryden MW, et al. Comparison of common fecal flotation techniques for the recovery of parasite eggs and oocysts. Vet Ther 2005;6:15\u201328.","journal-title":"Vet Ther"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2009.08.001"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1177\/10406387231189193"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1177\/10406387221129259"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1645\/13-197.1"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2009.07.044"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.16012"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-021-04678-6"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-020-04319-4"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10406387251385940","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/10406387251385940","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10406387251385940","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:29:21Z","timestamp":1777681761000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/10406387251385940"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,3]]},"references-count":13,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1177\/10406387251385940"],"URL":"https:\/\/doi.org\/10.1177\/10406387251385940","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,3]]}}}