{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T11:41:31Z","timestamp":1785498091752,"version":"3.56.0"},"reference-count":39,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T00:00:00Z","timestamp":1768953600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:p>Feline coronavirus (FCoV) infects both domestic and wild felids and has the potential to cause feline infectious peritonitis (FIP), a progressive and often fatal systemic disease. Although rapid diagnosis and treatment are crucial in cases of FIP, conventional reverse-transcription quantitative real-time PCR (RT-qPCR) requires RNA extraction and specialized equipment, limiting its use for timely testing in general veterinary practice. We evaluated the performance of a direct RT-qPCR method using the PicoGene PCR1100 system (GoFoton, Ibaraki, Japan), which omits the RNA extraction step and delivers results within ~40\u2009min. Compared with FCoV culture supernatants and extracted RNA, we estimated the limit of detection of this direct RT-qPCR method to be 150 copies\/reaction\u2014a detection sensitivity equivalent to that of conventional RT-qPCR targeting the FCoV 3\u2032-UTR. We observed no cross-reactivity with other feline viruses or SARS-CoV-2. We subsequently analyzed 28 pleural and abdominal effusions collected from cats suspected of having FIP to compare the direct RT-qPCR method with the conventional approach. The sensitivity of the direct RT-qPCR method was 95.5% (95% CI: [78.2, 99.2]) and the specificity was 100% (95% CI: [61.0, 100.0]), which supports the use of the PCR1100 system as a rapid and user-friendly point-of-care tool for the detection of FCoV RNA in effusion samples.<\/jats:p>","DOI":"10.1177\/10406387251411275","type":"journal-article","created":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T10:08:36Z","timestamp":1768990116000},"page":"184-191","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Clinical evaluation of a direct RT-qPCR method for feline coronavirus detection in effusions using the PicoGene PCR1100 system"],"prefix":"10.1177","volume":"38","author":[{"given":"Tomoyoshi","family":"Doki","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Aomori, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoko","family":"Yonezawa","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Aomori, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masato","family":"Katayama","sequence":"additional","affiliation":[{"name":"Bloom Animal Hospital, Tsurumi, Yokohama City, Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaito","family":"To","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Aomori, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3468-0708","authenticated-orcid":false,"given":"Tomomi","family":"Takano","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Aomori, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,1,21]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.19129-0"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-025-09340-0"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-9373-7_12"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/v17020279"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1177\/1098612X15574757"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-020-04605-7"},{"key":"e_1_3_3_8_2","first-page":"81","volume-title":"Coronaviruses Molecular and Cellular Biology","author":"Enjuanes L.","year":"2007","unstructured":"Enjuanes L. 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