{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:42:37Z","timestamp":1777930957846,"version":"3.51.4"},"reference-count":17,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T00:00:00Z","timestamp":1769644800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:p>\n                    A 4-y-old, male English Mastiff with a history of inflammatory neuromuscular disease developed progressive tetraparesis, ataxia, and severe temporal muscle atrophy, culminating in non-ambulatory status and euthanasia. The autopsy revealed diffuse muscle atrophy and pale pulmonary nodules. Histologically, polyphasic myositis was evident, with apicomplexan organisms within skeletal muscle myofibers. Encephalomyelitis, myocarditis, and hepatitis with protozoal cysts and tachyzoites also were observed. Immunohistochemistry of brain tissue was strongly positive for\n                    <jats:italic toggle=\"yes\">Neospora caninum<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Toxoplasma gondii<\/jats:italic>\n                    , but PCR testing confirmed\n                    <jats:italic toggle=\"yes\">N. caninum<\/jats:italic>\n                    and excluded\n                    <jats:italic toggle=\"yes\">T. gondii<\/jats:italic>\n                    , establishing a diagnosis of disseminated neosporosis. Despite extensive tissue involvement,\n                    <jats:italic toggle=\"yes\">N. caninum<\/jats:italic>\n                    antibody titers were below the diagnostic cutoff. Our case highlights that non-positive serologic results do not exclude neosporosis in clinically compatible cases, particularly in dogs undergoing corticoid therapy, and underscores the diagnostic value of histopathology with confirmatory molecular testing for definitive diagnosis.\n                  <\/jats:p>","DOI":"10.1177\/10406387261417402","type":"journal-article","created":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T07:28:31Z","timestamp":1769758111000},"page":"555-558","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Seronegative disseminated neosporosis with fatal outcome in an adult dog following prednisone therapy"],"prefix":"10.1177","volume":"38","author":[{"given":"Constanza L.","family":"Rodriguez","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine, Universidad Austral de Chile, Valdivia, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kayla","family":"Fowler","sequence":"additional","affiliation":[{"name":"Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, VA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin","family":"Lahmers","sequence":"additional","affiliation":[{"name":"Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, VA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle","family":"Todd","sequence":"additional","affiliation":[{"name":"Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, VA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rell L.","family":"Parker","sequence":"additional","affiliation":[{"name":"Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, VA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0681-1878","authenticated-orcid":false,"given":"Francisco A.","family":"Uzal","sequence":"additional","affiliation":[{"name":"California Animal Health and Food Safety Laboratory, School of Veterinary Medicine, University of California\u2013Davis, San Bernardino, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6178-4801","authenticated-orcid":false,"given":"Javier","family":"Asin","sequence":"additional","affiliation":[{"name":"California Animal Health and Food Safety Laboratory, School of Veterinary Medicine, University of California\u2013Davis, San Bernardino, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5115-9949","authenticated-orcid":false,"given":"Francisco R.","family":"Carvallo-Chaigneau","sequence":"additional","affiliation":[{"name":"Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, VA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2026,1,29]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.17145"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.139.18.439"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00942230"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/1040638720958467"},{"key":"e_1_3_3_6_2","first-page":"544","article-title":"Disseminated Neospora caninum encephalomyelitis and myositis in a 3-month-old Cane Corso puppy","volume":"11","author":"English AL","year":"2024","unstructured":"English AL, et al Disseminated Neospora caninum encephalomyelitis and myositis in a 3-month-old Cane Corso puppy. Vet Sci 2024;11:544.","journal-title":"Vet Sci"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(01)00493-9"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.2460\/javma.1995.206.07.1000"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182006000485"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/vms3.70425"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2006.tb00121.x"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.17219"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2015.03.005"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063870101300312"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.tcam.2010.07.005"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12287"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.2307\/3284407"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4894(03)00033-X"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10406387261417402","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/10406387261417402","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10406387261417402","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:29:50Z","timestamp":1777681790000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/10406387261417402"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,29]]},"references-count":17,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1177\/10406387261417402"],"URL":"https:\/\/doi.org\/10.1177\/10406387261417402","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,29]]}}}