{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T19:58:28Z","timestamp":1791403108282,"version":"4.3.3"},"reference-count":44,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T00:00:00Z","timestamp":1772582400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T00:00:00Z","timestamp":1772582400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100017545","name":"Institute of Veterinary Bacteriology, University of Bern","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017545","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:p>\n                    Three\n                    <jats:italic toggle=\"yes\">Treponema<\/jats:italic>\n                    sp.\u2014\n                    <jats:italic toggle=\"yes\">T. medium<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">T. pedis<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">T. phagedenis\u2014<\/jats:italic>\n                    are associated with bovine digital dermatitis (BDD). This painful infectious disease has been reported worldwide, causing economic and animal welfare problems. Although detection by real-time PCR (rtPCR) is the method of choice, published assays differ in specificity and sensitivity. We multiplexed target regions of 2 published rtPCR assays and slightly optimized another one to compare their performance. We then used the 2 novel TaqMan rtPCR assays to determine the prevalence of the 3\n                    <jats:italic toggle=\"yes\">Treponema<\/jats:italic>\n                    sp. in a random sample of hind feet from 137 animals collected at slaughterhouses. An overall prevalence at the animal level of 31.4% was found for\n                    <jats:italic toggle=\"yes\">T. phagedenis<\/jats:italic>\n                    and 10.2% for\n                    <jats:italic toggle=\"yes\">T. medium<\/jats:italic>\n                    , with the latter always being detected together with\n                    <jats:italic toggle=\"yes\">T. phagedenis<\/jats:italic>\n                    . We did not detect\n                    <jats:italic toggle=\"yes\">T. pedis<\/jats:italic>\n                    in our sample set. Typical lesions of BDD were present in 16.2% of animals. Of the animals with BDD lesions, 52.6% were positive for any of the 3\n                    <jats:italic toggle=\"yes\">Treponema<\/jats:italic>\n                    sp. A significantly lower positive rate for\n                    <jats:italic toggle=\"yes\">Treponema<\/jats:italic>\n                    sp. was found in animals without lesions, underscoring the role of these organisms in the development of BDD. Our study offers insight into the prevalence of BDD-associated\n                    <jats:italic toggle=\"yes\">Treponema<\/jats:italic>\n                    sp. in cattle as well as a tool for routine diagnosis and future control of the disease.\n                  <\/jats:p>","DOI":"10.1177\/10406387261424230","type":"journal-article","created":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T15:41:50Z","timestamp":1773243710000},"page":"451-457","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Detection of\n                    <i>Treponema<\/i>\n                    species associated with bovine digital dermatitis in cattle using novel real-time PCR assays"],"prefix":"10.1177","volume":"38","author":[{"given":"Laura","family":"Tassera","sequence":"first","affiliation":[{"name":"Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Brodard","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3790-5746","authenticated-orcid":false,"given":"J\u00f6rg","family":"Jores","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1575-5225","authenticated-orcid":false,"given":"Peter","family":"Kuhnert","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,3,4]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13028-022-00635-0"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0178349"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1024\/0036-7281\/a000553"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-018-0605-z"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2012.06.048"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.livprodsci.2004.12.005"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2000.61.928"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2010.07.011"},{"key":"e_1_3_3_10_2","first-page":"208","volume-title":"Proc 8th Int Conf Dis Cattle","author":"Cheli R","year":"1974","unstructured":"Cheli R, Mortellaro C. La dermatite digitale del bovino [Digital dermatitis in cattle]. In: Proc 8th Int Conf Dis Cattle; Piacenza, Italy; 1974:208\u2013213. 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