{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T11:05:14Z","timestamp":1789297514968,"version":"build-2803163510"},"reference-count":22,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T00:00:00Z","timestamp":1775433600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Program for Science & Technology Innovation Talents in Universities of Henan Province","award":["24HASTIT061"],"award-info":[{"award-number":["24HASTIT061"]}]},{"name":"Scientific and Technological Project in Henan Province","award":["242102110058\/232102111048\/242102110002"],"award-info":[{"award-number":["242102110058\/232102111048\/242102110002"]}]},{"name":"Henan Provincial Excellent Young Scientist Foundation Project","award":["252300421155"],"award-info":[{"award-number":["252300421155"]}]},{"name":"Key Discipline of Veterinary Medicine of Henan Province","award":["312"],"award-info":[{"award-number":["312"]}]},{"name":"Henan Institute of Animal Husbandry and Economics","award":["2023HNUAHEDF003"],"award-info":[{"award-number":["2023HNUAHEDF003"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:p>\n                    Goose astrovirus 1 (GAstV1), goose astrovirus 2 (GAstV2), and goose parvovirus (GPV) infections have significant negative effects on the goose farming industry. A rapid and accurate multiplex PCR method for simultaneous detection of these 3 goose viruses is needed. We designed and synthesized specific primers targeting the conserved regions of GAstV1, GAstV2, and GPV. and optimized reaction conditions to assess specificity and sensitivity. The optimal amplification condition was an annealing temperature of 54.5\u00b0C for 35 cycles. Specific bands were detected at 315\u2009bp (GAstV1), 609\u2009bp (GAstV2), and 1,405\u2009bp (GPV). No cross-amplification occurred with other common goose pathogens, including avian influenza A virus H9, Newcastle disease virus, duck Tembusu virus, fowl adenovirus 4, goose reovirus, and goose circovirus. The limit of detection (LOD) for GAstV1, GAstV2, and GPV was 1\u2009\u00d7\u200910\n                    <jats:sup>3<\/jats:sup>\n                    copies\/\u03bcL, and the LOD for samples containing all 3 agents was 1\u2009\u00d7\u200910\n                    <jats:sup>4<\/jats:sup>\n                    copies\/\u03bcL. Our results from testing 90 clinical samples were in 100% concordance with single PCR assays, suggesting that our multiplex PCR assay offers a reliable and efficient approach for the clinical detection of GAstV1, GAstV2, and GPV.\n                  <\/jats:p>","DOI":"10.1177\/10406387261436326","type":"journal-article","created":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T13:59:47Z","timestamp":1775483987000},"page":"956-962","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Development and preliminary application of a multiplex PCR method for the detection of goose parvovirus and goose astrovirus 1 and 2"],"prefix":"10.1177","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-4504-1010","authenticated-orcid":false,"given":"Xinghui","family":"Song","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengkun","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyue","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Cao","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenzhuo","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhifeng","family":"Peng","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanling","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaozhan","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,4,6]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2021.101407"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2020.09.077"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.14699"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-021-05086-y"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/03079457708418242"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2023.102730"},{"key":"e_1_3_2_8_2","first-page":"141","article-title":"Isolation of novel goose astrovirus, FJ-NP","volume":"36","author":"Lin YS","year":"2021","unstructured":"Lin YS, et al. 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