{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T10:48:07Z","timestamp":1791802087101,"version":"4.3.4"},"reference-count":35,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:00:00Z","timestamp":1783036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:00:00Z","timestamp":1783036800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100022314","name":"Center for Veterinary Medicine","doi-asserted-by":"publisher","award":["HHSF223201410256A"],"award-info":[{"award-number":["HHSF223201410256A"]}],"id":[{"id":"10.13039\/100022314","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100022314","name":"Center for Veterinary Medicine","doi-asserted-by":"publisher","award":["U18FD005013"],"award-info":[{"award-number":["U18FD005013"]}],"id":[{"id":"10.13039\/100022314","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100022314","name":"Center for Veterinary Medicine","doi-asserted-by":"publisher","award":["U18FD006446"],"award-info":[{"award-number":["U18FD006446"]}],"id":[{"id":"10.13039\/100022314","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100022314","name":"Center for Veterinary Medicine","doi-asserted-by":"publisher","award":["U18FD006664"],"award-info":[{"award-number":["U18FD006664"]}],"id":[{"id":"10.13039\/100022314","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100022314","name":"Center for Veterinary Medicine","doi-asserted-by":"publisher","award":["U18FD007242"],"award-info":[{"award-number":["U18FD007242"]}],"id":[{"id":"10.13039\/100022314","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:p>\n                    We evaluated a semi-automated method for DNA extraction, expanded to herbivore feces a\n                    <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                    PCR method for the detection of\n                    <jats:italic toggle=\"yes\">Campylobacter jejuni<\/jats:italic>\n                    in canine feces, added an internal positive control (IPC) DNA to specimens before DNA extraction, and multiplexed the\n                    <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                    PCR assay with the IPC assay. The addition of IPC DNA and multiplexing of the IPC assay did not affect the performance of the\n                    <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                    PCR assay. The method was repeatable with no significant difference in results across 6\u2009d (F\u2009=\u20090.715;\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009=\u20090.614 with\n                    <jats:italic toggle=\"yes\">R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u2009=\u20090.818). In a single-laboratory randomized method test evaluating detection in bovine feces, sensitivity was 96.1%, specificity was 100%, and accuracy was 94.1%. In a multi-laboratory randomized method test (M-RMT) in bovine feces, agreement was 90% between laboratories on test results with \u03ba\u2009=\u20090.80 (95% CI [0.64, 0.96]). Comparison of manual DNA extraction kits with a semi-automated extraction method in canine feces found 88.9% agreement, with \u03ba\u2009=\u20090.78 (95% CI [0.64, 0.92]). In a second M-RMT in canine feces, accuracy was 78% with 90% agreement between the laboratories and \u03ba\u2009=\u20090.80 (95% CI [0.64, 0.96]), even though inoculation of the intended fractional level was lower than intended at 45 cfu\/g feces. Overall, the\n                    <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                    PCR assay was extended to an additional matrix, the inclusion of an IPC DNA and assay was successful, and the\n                    <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                    PCR assay for the detection of\n                    <jats:italic toggle=\"yes\">Campylobacter jejuni<\/jats:italic>\n                    provided repeatable results in multiple laboratories.\n                  <\/jats:p>","DOI":"10.1177\/10406387261454377","type":"journal-article","created":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T10:42:35Z","timestamp":1783075355000},"page":"1170-1181","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Matrix extension to bovine feces and evaluation of semi-automated DNA extraction methods for the detection of\n                    <i>Campylobacter jejuni<\/i>\n                    in bovine and canine fecal samples using\n                    <i>gyrA<\/i>\n                    PCR"],"prefix":"10.1177","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9154-8909","authenticated-orcid":false,"given":"Sara D.","family":"Lawhon","sequence":"first","affiliation":[{"name":"Veterinary Pathobiology, Texas A&amp;M University, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wu","sequence":"additional","affiliation":[{"name":"Veterinary Medical Teaching Hospital, Texas A&amp;M University, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7741-8497","authenticated-orcid":false,"given":"Kristy L.","family":"Pabilonia","sequence":"additional","affiliation":[{"name":"Colorado State University Veterinary Diagnostic Laboratory, Ft. 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