{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T08:07:55Z","timestamp":1789373275337,"version":"build-2803163510"},"reference-count":34,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"abstract":"<jats:p>\n                    Pathogen infections and coinfections are critical determinants of feline upper respiratory tract disease; although the etiologic agents are diverse, their clinical manifestations often overlap. Established detection panels for feline upper respiratory tract infections (FURTIs) primarily focus on core pathogens\u2014feline calicivirus (FCV), felid alphaherpesvirus 1 (FeAHV1),\n                    <jats:italic toggle=\"yes\">Mycoplasmopsis felis<\/jats:italic>\n                    (MF),\n                    <jats:italic toggle=\"yes\">Chlamydia felis<\/jats:italic>\n                    (CF),\n                    <jats:italic toggle=\"yes\">Bordetella bronchiseptica<\/jats:italic>\n                    (BB), and influenza A virus (IAV)\u2014and frequently do not incorporate emerging zoonotic agents (e.g., SARS-CoV-2) and overlooked streptococcal species (\n                    <jats:italic toggle=\"yes\">Streptococcus equi<\/jats:italic>\n                    subsp.\n                    <jats:italic toggle=\"yes\">zooepidemicus<\/jats:italic>\n                    [SEZ] and\n                    <jats:italic toggle=\"yes\">S. canis<\/jats:italic>\n                    [SC]) in a multiplex assay. Furthermore, simultaneous quantification remains technically challenging. We developed an integrated multiplex reverse-transcription PCR (RT-PCR)\/PCR\u2013capillary electrophoresis (CE) platform for simultaneous quantitative detection of 9 feline respiratory pathogens (FCV, FeAHV1, MF, CF, BB, IAV, SARS-CoV-2, SEZ, SC). Requiring only a standard thermal cycler and a basic CE instrument, our platform is highly accessible to resource-limited laboratories, offering high specificity and a 1.6-h turnaround time. With a limit of detection of 1 copy\/\u00b5L across all targets, our multiplex assay had exceptional sensitivity, surpassing conventional singleplex RT-PCR\/PCR on both agarose gel and real-time PCR platforms. Notably, we achieved a 2.2-fold higher detection rate than the conventional gold standard (singleplex RT-PCR\/PCR with agarose gel). This enhanced sensitivity enabled us to identify even low-pathogen\u2013load samples, often missed by conventional assays. When used with clinical specimens, the platform offered comprehensive coverage of major and emerging zoonotic FURTI pathogens, confirming its potential as a cost-effective, high-efficiency tool for differential diagnosis, targeted therapy, and epidemiologic surveillance.\n                  <\/jats:p>","DOI":"10.1177\/10406387261484040","type":"journal-article","created":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T04:47:08Z","timestamp":1789188428000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Quantitative multiplex detection of 9 feline respiratory pathogens with enhanced sensitivity: a cost-effective integrated platform combining RT-PCR\/PCR and capillary electrophoresis"],"prefix":"10.1177","author":[{"given":"Chun-An","family":"Chen","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han-Tso","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Ming Chuan University, Taoyuan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-5656-2390","authenticated-orcid":false,"given":"Shi-Wen","family":"Goh","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Han-You Lin","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7302-4714","authenticated-orcid":false,"given":"Lih-Chiann","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,9,11]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.7853\/kjvs.2023.46.4.269"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfms.2004.07.004"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.00257-22"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.12.040"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2009.12.007"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2004.01.014"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2011.07.006"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2020.25.3.2000045"},{"key":"e_1_3_2_10_2","article-title":"First case of zoonotic transmission of Streptococcus equi subspecies zooepidemicus from cat to human","volume":"15","author":"Dolapsakis C","year":"2023","unstructured":"Dolapsakis C, et al. 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