{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T07:54:32Z","timestamp":1790927672137,"version":"4.1.0"},"reference-count":11,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Guangxi Basic Research and Development Program","award":["Guike HJ2600640003"],"award-info":[{"award-number":["Guike HJ2600640003"]}]},{"DOI":"10.13039\/501100004607","name":"natural science foundation of guangxi province","doi-asserted-by":"publisher","award":["2023GXNSFDA026042"],"award-info":[{"award-number":["2023GXNSFDA026042"]}],"id":[{"id":"10.13039\/501100004607","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guangxi Nanning Heyi Biological Technology"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Chlamydia felis<\/jats:italic>\n                    is a primary cause of feline conjunctivitis and upper respiratory tract disease. Detection of\n                    <jats:italic toggle=\"yes\">C. felis<\/jats:italic>\n                    largely relies on molecular tests; however, these tests cannot assess prior exposure or herd immunity. In China, no standardized commercial serologic assay for\n                    <jats:italic toggle=\"yes\">C. felis<\/jats:italic>\n                    is available. We developed an indirect ELISA based on the major outer membrane protein (MOMP) of a locally isolated\n                    <jats:italic toggle=\"yes\">C. felis<\/jats:italic>\n                    strain (GXNN36). A truncated\n                    <jats:italic toggle=\"yes\">ompA<\/jats:italic>\n                    gene (encoding MOMP, amino acids 22\u2013392, which retains the major antigenic domains, including the 4 variable domains VD1\u20134) was expressed in\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    , and the recombinant protein was purified. After optimizing reaction conditions, the iELISA had high specificity (no cross-reactivity with feline calicivirus, panleukopenia virus, or feline coronavirus), high sensitivity (detection limit up to 1:20,480 dilution), and good reproducibility (intra- and inter-assay CVs &lt;10%). The cutoff optical density (OD450) was 0.630. Assessment against western blotting using 20 clinical sera had 95% concordance. Testing of 144 feline serum samples from Guangxi, China revealed a seroprevalence of 20.1%. Our MOMP-based iELISA using a Chinese\n                    <jats:italic toggle=\"yes\">C. felis<\/jats:italic>\n                    isolate offers a practical and regionally relevant tool for serosurveillance.\n                  <\/jats:p>","DOI":"10.1177\/10406387261485570","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T10:32:55Z","timestamp":1790591575000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of an indirect ELISA based on the major outer membrane protein for detection of antibodies against\n                    <i>Chlamydia felis<\/i>"],"prefix":"10.1177","author":[{"given":"Haodong","family":"Zhang","sequence":"first","affiliation":[{"name":"Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rang","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Dong","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Xue","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengjun","family":"Xiang","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolong","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongmei","family":"Pan","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoli","family":"Yang","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huabo","family":"Zhou","sequence":"additional","affiliation":[{"name":"Huabo Pet Hospital, Nanning, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifeng","family":"Qin","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, PR China"},{"name":"Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, PR China"},{"name":"Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang","family":"Ouyang","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, PR China"},{"name":"Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, PR China"},{"name":"Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeshi","family":"Yin","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, PR China"},{"name":"Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, PR China"},{"name":"Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuzhang","family":"Wei","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, PR China"},{"name":"Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, PR China"},{"name":"Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weijian","family":"Huang","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, PR China"},{"name":"Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, PR China"},{"name":"Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianming","family":"Long","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, PR China"},{"name":"Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, PR China"},{"name":"Guangxi Nanning Heyi Biological Technology, Nanning, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6720-353X","authenticated-orcid":false,"given":"Ying","family":"Chen","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, PR China"},{"name":"Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, PR China"},{"name":"Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,9,28]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2025.1502642"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2024.110128"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci10010046"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmni.2024.101412"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-024-06093-5"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.33073\/pjm-2023-029"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0224070"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2010.05.026"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1110\/ps.3650102"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens13090771"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.3390\/ani14111562"}],"container-title":["Journal 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