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A mechanically extracted membrane and a lipopolysaccharide antigen showed a high degree of leptospiral serogroup specificity. The lipopolysaccharide antigen was the most suitable antigen for detection of anti-hardjo antibodies. Enzyme immunoassay was more sensitive than the microscopic agglutination test for detecting antibodies in serum from experimentally and naturally infected cattle. It was not possible to differentiate vaccinated from infected animals or to detect a secondary immune response in vaccinated animals that were subsequently infected.<\/jats:p>","DOI":"10.1177\/104063879000200413","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T16:10:00Z","timestamp":1305216600000},"page":"323-329","source":"Crossref","is-referenced-by-count":15,"title":["Evaluation of an Enzyme Immunoassay for Diagnosis of Bovine Leptospirosis Caused by\n                    <i>Leptospira Interrogans<\/i>\n                    Serovar\n                    <i>Hardjo<\/i>\n                    Type Hardjo-Bovis"],"prefix":"10.1177","volume":"2","author":[{"given":"Gabriel A.","family":"Trueba","sequence":"first","affiliation":[{"name":"Leptospirosis and Mycobacteriosis Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, PO Box 70, Ames, IA 50010"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carole A.","family":"Bolin","sequence":"additional","affiliation":[{"name":"Leptospirosis and Mycobacteriosis Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, PO Box 70, Ames, IA 50010"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles O.","family":"Thoen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA 50011"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1990,10,1]]},"reference":[{"key":"bibr1-104063879000200413","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-11-4-401"},{"key":"bibr2-104063879000200413","doi-asserted-by":"publisher","DOI":"10.3109\/00313028309083501"},{"key":"bibr3-104063879000200413","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1981.tb00546.x"},{"key":"bibr4-104063879000200413","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.11.5.452-457.1980"},{"key":"bibr5-104063879000200413","doi-asserted-by":"publisher","DOI":"10.1128\/iai.5.6.968-975.1972"},{"key":"bibr6-104063879000200413","doi-asserted-by":"publisher","DOI":"10.1016\/0147-9571(83)90038-3"},{"key":"bibr7-104063879000200413","doi-asserted-by":"crossref","first-page":"161","DOI":"10.2460\/ajvr.1989.50.01.161","volume":"50","author":"Bolin CA","year":"1989","journal-title":"Am J Vet Res"},{"key":"bibr8-104063879000200413","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.2460\/ajvr.1989.50.07.1001","volume":"50","author":"Bolin CA","year":"1989","journal-title":"Am J Vet Res"},{"key":"bibr9-104063879000200413","doi-asserted-by":"crossref","first-page":"2004","DOI":"10.2460\/ajvr.1989.50.12.2004","volume":"50","author":"Bolin CA","year":"1989","journal-title":"Am J Vet Res"},{"key":"bibr10-104063879000200413","first-page":"277","volume":"269","author":"Cinco M","year":"1988","journal-title":"Zentralbl Bakteriol Mikrobiol Hyg Ser A"},{"key":"bibr11-104063879000200413","doi-asserted-by":"publisher","DOI":"10.1128\/am.25.6.976-980.1973"},{"key":"bibr12-104063879000200413","first-page":"323","volume":"43","author":"Corvoz L","year":"1987","journal-title":"Isr J Vet Med"},{"key":"bibr13-104063879000200413","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(85)90026-4"},{"key":"bibr14-104063879000200413","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.34.10.1128"},{"key":"bibr15-104063879000200413","doi-asserted-by":"publisher","DOI":"10.1021\/ac60111a017"},{"key":"bibr16-104063879000200413","first-page":"39","volume":"26","author":"Ellinghausen HC","year":"1965","journal-title":"Am J Vet Res"},{"key":"bibr17-104063879000200413","unstructured":"Ellis WA, Thiermann AB, Marshall RB: 1986, Genotypes of Leptospira hardjo and their role in clinical disease. 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