{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:43:37Z","timestamp":1777931017000,"version":"3.51.4"},"reference-count":21,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[1992,1,1]],"date-time":"1992-01-01T00:00:00Z","timestamp":694224000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[1992,1]]},"abstract":"<jats:p>\n                    Diagnosis of paratuberculosis using the IDEXX DNA probe test and 3 methods for cultivation of Mycobacterium paratuberculosis from fecal specimens were compared. Twenty-one of 170 fecal specimens were DNA probe test positive, whereas 35 specimens were positive by 1 or more of the cultivation methods evaluated. Four specimens were DNA probe test positive but were negative by fecal culture. The probe test detected M. paratuberculosis DNA in 62.9% of the specimens positive by a sedimentation culture method, in 56.6% of those positive by a centrifugation culture method, and in 65.4% of the specimens positive by the Cornell culture method. Specificity of the DNA probe test was approximately 97% relative to all culture methods. Generally, the probe test detected M. paratuberculosis DNA in fecal specimens from animals shedding at least 10\n                    <jats:sup>4<\/jats:sup>\n                    M. paratuberculosis colony forming units per gram of feces. Although the probe test did not detect all of the cattle shedding M. paratuberculosis, it was possible to identify cattle shedding the greatest number of organisms in 3 days compared with a minimum of 6 weeks required for positive culture results. The centrifugation method resulted in the most isolations of M. paratuberculosis after 12 weeks of incubation. However, contamination also was greatest when the centrifugation method was used. Contamination was best controlled using the Cornell method. The sedimentation method was the least time consuming and yielded results similar to those of the other 2 methods.\n                  <\/jats:p>","DOI":"10.1177\/104063879200400106","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T16:10:00Z","timestamp":1305216600000},"page":"23-27","source":"Crossref","is-referenced-by-count":47,"title":["Comparison of a Commercial DNA Probe Test and Three Cultivation Procedures for Detection of\n                    <i>Mycobacterium Paratuberculosis<\/i>\n                    in Bovine Feces"],"prefix":"10.1177","volume":"4","author":[{"given":"Diana L.","family":"Whipple","sequence":"first","affiliation":[{"name":"Leptospirosis\/Mycobacteriosis Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul A.","family":"Kapke","sequence":"additional","affiliation":[{"name":"Leptospirosis\/Mycobacteriosis Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phil R.","family":"Andersen","sequence":"additional","affiliation":[{"name":"IDEXX Corp., Portland, ME 04101"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1992,1,1]]},"reference":[{"key":"bibr1-104063879200400106","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(91)90044-G"},{"key":"bibr2-104063879200400106","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1990.tb07693.x"},{"key":"bibr3-104063879200400106","first-page":"218","volume":"74","author":"Chiodini RJ","year":"1984","journal-title":"Cornell Vet"},{"key":"bibr4-104063879200400106","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(89)90083-7"},{"key":"bibr5-104063879200400106","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1989.tb03440.x"},{"key":"bibr6-104063879200400106","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/17.22.9063"},{"key":"bibr7-104063879200400106","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.27.7.1582-1587.1989"},{"key":"bibr8-104063879200400106","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.2460\/ajvr.1989.50.07.1110","volume":"50","author":"Kim YG","year":"1989","journal-title":"Am J Vet Res"},{"key":"bibr9-104063879200400106","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(88)90487-4"},{"key":"bibr10-104063879200400106","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.25.5.796-801.1987"},{"key":"bibr11-104063879200400106","doi-asserted-by":"publisher","DOI":"10.1080\/00480169.1989.35556"},{"key":"bibr12-104063879200400106","doi-asserted-by":"publisher","DOI":"10.1126\/science.2448875"},{"key":"bibr13-104063879200400106","first-page":"299","volume":"197","author":"Sanftleben P","year":"1990","journal-title":"J Am Vet Med Assoc"},{"key":"bibr14-104063879200400106","first-page":"381","volume":"93","author":"Shin S","year":"1989","journal-title":"Proc Annu Meet US Anim Health Assoc"},{"key":"bibr15-104063879200400106","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.28.5.933-937.1990"},{"key":"bibr16-104063879200400106","doi-asserted-by":"publisher","DOI":"10.1177\/104063879100300424"},{"key":"bibr17-104063879200400106","unstructured":"Whipple DL, Merkal RS: 1983, Modifications in the techniques for cultivation of Mycobacterium paratuberculosis. Proc Int Colloq Res Paratuberculosis, Ames, IA, pp. 82\u201392."},{"key":"bibr18-104063879200400106","first-page":"475","volume":"89","author":"Whipple DL","year":"1985","journal-title":"Proc Annu Meet US Anim Health Assoc"},{"key":"bibr19-104063879200400106","first-page":"382","volume":"93","author":"Whitlock RH","year":"1989","journal-title":"Proc Annu Meet US Anim Health Assoc"},{"key":"bibr20-104063879200400106","unstructured":"Whitlock RH, Sweeney RW: 1990, Johne's disease: Current aspects of transmission and the art of fecal culture testing. Proc Annu Meet Livestock Conserv Inst, pp. 24\u201330."},{"key":"bibr21-104063879200400106","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711620203"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063879200400106","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063879200400106","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:31:58Z","timestamp":1777681918000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/104063879200400106"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,1]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1992,1]]}},"alternative-id":["10.1177\/104063879200400106"],"URL":"https:\/\/doi.org\/10.1177\/104063879200400106","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,1]]}}}