{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:44:19Z","timestamp":1777931059536,"version":"3.51.4"},"reference-count":23,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[1993,10,1]],"date-time":"1993-10-01T00:00:00Z","timestamp":749433600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[1993,10]]},"abstract":"<jats:p>\n                    Fifteen bovine herpesvirus-1 (BHV-1)-negative calves were vaccinated intramuscularly with 10\n                    <jats:sup>7.4<\/jats:sup>\n                    plaque-forming units of a double-deletion BHV-1 mutant (IBRV(NG)dltkdlgIII), and 6 remained as nonvaccinated controls. Thirty days after vaccination, the animals were challenged by nasal instillation of 10\n                    <jats:sup>8.2<\/jats:sup>\n                    CCID\n                    <jats:sub>50<\/jats:sub>\n                    of a virulent BHV-1 strain (Cooper). The vaccinated calves were protected against wildtype virus challenge as demonstrated by clinical evaluation. Most of the vaccinates developed only a mild rhinitis (lasting an average of 6.5 days) with almost no systemic symptoms, whereas the controls developed a serious illness characterized by rhinitis (X = 11.5 days), conjunctivitis, hyperthermia, apathy, loss of appetite, and dyspnea. The vaccinates also shed significantly less virus and for a shorter period of time (X = 5.5 days) than the controls (X = 9 days). Thirty days after vaccination, the vaccinates were negative in an anti-gIII specific blocking enzyme-linked immunosorbent assay (ELISA), despite the fact that most of them had developed neutralizing antibodies (serum neutralization titers ranging from 1:2 to 1: 16). Seroconversion to gIII was detected as early as 7 days postinfection (dpi). Fourteen days after the challenge, all the animals exposed to wildtype BHV-1 had developed anti-gIII antibodies and were positive in this differential serologic test. Six controls plus 8 vaccinates kept in isolation were still positive to gIII when tested at 75 dpi. The use of the IBRV(NG)dltkdlgIII strain in conjunction with an anti-gIII specific blocking ELISA kit represents a powerful tool for BHV-1 control\/eradication programs.\n                  <\/jats:p>","DOI":"10.1177\/104063879300500406","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T16:10:00Z","timestamp":1305216600000},"page":"534-540","source":"Crossref","is-referenced-by-count":26,"title":["Efficacy of a Deletion Mutant Bovine Herpesvirus-1 (BHV-1) Vaccine That Allows Serologic Differentiation of Vaccinated from Naturally Infected Animals"],"prefix":"10.1177","volume":"5","author":[{"given":"Eduardo Furtado","family":"Flores","sequence":"first","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, NE 68583"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando A.","family":"Osorio","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, NE 68583"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eraldo L.","family":"Zanella","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, NE 68583"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saul","family":"Kit","sequence":"additional","affiliation":[{"name":"Division of Biochemical Virology, Baylor College of Medicine, Houston, TX 77030"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malon","family":"Kit","sequence":"additional","affiliation":[{"name":"Novagene, Inc., Houston, TX 77027"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1993,10,1]]},"reference":[{"key":"bibr1-104063879300500406","doi-asserted-by":"crossref","first-page":"36","DOI":"10.2460\/ajvr.1982.43.01.36","volume":"43","author":"Ackermann M","year":"1982","journal-title":"Am J Vet Res"},{"key":"bibr2-104063879300500406","doi-asserted-by":"publisher","DOI":"10.1136\/vr.111.6.116"},{"key":"bibr3-104063879300500406","doi-asserted-by":"crossref","first-page":"893","DOI":"10.2460\/ajvr.1985.46.04.893","volume":"46","author":"Guy JS","year":"1985","journal-title":"Am J Vet Res"},{"key":"bibr4-104063879300500406","volume-title":"Serologic microtitration techniques","author":"Jenney EW","year":"1981"},{"key":"bibr5-104063879300500406","first-page":"1055","volume":"171","author":"Kahrs RF","year":"1977","journal-title":"J Am Vet Med Assoc"},{"key":"bibr6-104063879300500406","first-page":"183","author":"Kit S","year":"1988","journal-title":"Tech Adv Vaccine Dev"},{"key":"bibr7-104063879300500406","first-page":"128","volume":"38","author":"Kit S","year":"1991","journal-title":"Prog Med Virol"},{"key":"bibr8-104063879300500406","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(86)90098-8"},{"key":"bibr9-104063879300500406","unstructured":"Kit S, Kit M, Otsuka H: 1990, US patent nos. 4992051 and 5128129. US Patents Office, Washington, DC."},{"key":"bibr10-104063879300500406","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(92)90006-Y"},{"key":"bibr11-104063879300500406","doi-asserted-by":"publisher","DOI":"10.1007\/BF01314114"},{"key":"bibr12-104063879300500406","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.65.3.1124-1132.1991"},{"key":"bibr13-104063879300500406","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.65.10.5553-5557.1991"},{"key":"bibr14-104063879300500406","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(92)90586-E"},{"key":"bibr15-104063879300500406","doi-asserted-by":"crossref","first-page":"383","DOI":"10.2460\/ajvr.1980.41.03.383","volume":"41","author":"Lupton HW","year":"1980","journal-title":"Am J Vet Res"},{"key":"bibr16-104063879300500406","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.57.3.745-753.1986"},{"key":"bibr17-104063879300500406","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(91)90900-V"},{"key":"bibr18-104063879300500406","doi-asserted-by":"publisher","DOI":"10.1177\/104063878900100209"},{"key":"bibr19-104063879300500406","first-page":"850","volume":"163","author":"SheffL BE","year":"1973","journal-title":"J Am Vet Med Assoc"},{"key":"bibr20-104063879300500406","doi-asserted-by":"publisher","DOI":"10.1136\/vr.122.17.407"},{"key":"bibr21-104063879300500406","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.23.2.274-282.1986"},{"key":"bibr22-104063879300500406","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.59.2.401-410.1986"},{"key":"bibr23-104063879300500406","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(90)90139-M"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063879300500406","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063879300500406","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:33:25Z","timestamp":1777682005000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/104063879300500406"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,10]]},"references-count":23,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1993,10]]}},"alternative-id":["10.1177\/104063879300500406"],"URL":"https:\/\/doi.org\/10.1177\/104063879300500406","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,10]]}}}