{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:44:43Z","timestamp":1777931083357,"version":"3.51.4"},"reference-count":12,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[1995,1,1]],"date-time":"1995-01-01T00:00:00Z","timestamp":788918400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[1995,1]]},"abstract":"<jats:p>A dot-blot assay and an enzyme-linked immunosorbent assay (ELISA) to detect listeriosis in dairy cattle were developed that detected anti-listeriolysin O antibodies in the serum of cows experimentally infected with Listeria monocytogenes. The tests utilized purified listeriolysin O (LLO) as the detection antigen and streptolysin O (SLO) to absorb cross-reacting antibodies. The two tests were compared with an agglutination test that used formalin-killed whole L. monocytogenes cells. Blood samples were collected periodically from 17 cows after intramammary gland infection, and the development of anti-LLO antibodies was followed by an agglutination test, the dot-blot test, and the ELISA. In general, an agglutination titer of &gt;640 was needed for a positive dot-blot anti-LLO test for nonpregnant cows. However, I pregnant cow with an agglutination titer of 20 was positive in the dot-blot test. The ELISA was as sensitive as the dot-blot assay but gave a quantitative measurement to distinguish serum samples of positive reactors from cross-reactors. The specificity of the LLO-based tests was further evaluated using serum from cows that had been experimentally infected with Staphylococcus aureus, 17 of which had agglutination titers for L. monocytogenes &gt;640. These elevated agglutination titers were probably due to cross-reacting bacterial antigens because serum from 9 of 17 of these animals did not react to the purified LLO antigen. A positive response to the LLO-based dot-blot and ELISA assays is indicative of previous or current infection with L. monocytogenes.<\/jats:p>","DOI":"10.1177\/104063879500700113","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T16:18:45Z","timestamp":1305217125000},"page":"82-86","source":"Crossref","is-referenced-by-count":15,"title":["Detection of Anti-Listeriolysin O in Dairy Cattle Experimentally Infected with\n                    <i>Listeria Monocytogenes<\/i>"],"prefix":"10.1177","volume":"7","author":[{"given":"Albert L.","family":"Baetz","sequence":"first","affiliation":[{"name":"Physiopathology Research Unit, National Animal Disease Center, US Department of Agriculture, Agriculture Research Service, PO Box 70, Ames, IA 50010"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene V.","family":"Wesley","sequence":"additional","affiliation":[{"name":"Physiopathology Research Unit, National Animal Disease Center, US Department of Agriculture, Agriculture Research Service, PO Box 70, Ames, IA 50010"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1995,1,1]]},"reference":[{"key":"bibr1-104063879500700113","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)90411-W"},{"key":"bibr2-104063879500700113","first-page":"137","volume":"36","author":"Bryner J","year":"1989","journal-title":"Acta Microbiol Hung"},{"key":"bibr3-104063879500700113","doi-asserted-by":"publisher","DOI":"10.1128\/iai.57.11.3629-3636.1989"},{"key":"bibr4-104063879500700113","doi-asserted-by":"publisher","DOI":"10.1128\/br.30.2.309-382.1966"},{"key":"bibr5-104063879500700113","first-page":"355","volume":"5","author":"Kvenberg JE","year":"1988","journal-title":"Microbiol Sci"},{"key":"bibr6-104063879500700113","doi-asserted-by":"publisher","DOI":"10.1016\/0034-5288(91)90012-D"},{"key":"bibr7-104063879500700113","doi-asserted-by":"publisher","DOI":"10.1136\/vr.116.6.147"},{"key":"bibr8-104063879500700113","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1991.tb04344.x"},{"key":"bibr9-104063879500700113","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.12.4641-4646.1991"},{"key":"bibr10-104063879500700113","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1992.03480150052036"},{"key":"bibr11-104063879500700113","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-55.2.93"},{"key":"bibr12-104063879500700113","first-page":"495","volume":"36","author":"Wesley IV","year":"1989","journal-title":"Acta Microbiol Hung"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063879500700113","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063879500700113","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:34:25Z","timestamp":1777682065000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/104063879500700113"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,1]]},"references-count":12,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1995,1]]}},"alternative-id":["10.1177\/104063879500700113"],"URL":"https:\/\/doi.org\/10.1177\/104063879500700113","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,1]]}}}