{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:45:33Z","timestamp":1777931133921,"version":"3.51.4"},"reference-count":19,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[1997,7,1]],"date-time":"1997-07-01T00:00:00Z","timestamp":867715200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[1997,7]]},"abstract":"<jats:p>An avidin-biotin complex immunohistochemistry technique was developed to detect Haemophilus parasuis serovar 5 in experimentally infected 18-21-day-old conventional pigs, using a rabbit polyclonal antiserum. Seven of 10 intratracheally inoculated animals developed a low to medium degree of fibrinous polyserositis; meninges and pleura were the most severely affected areas. Haemophilus parasuis was recovered from 9 of 10 pigs; in 2 of them H. parasuis was isolated from tracheal swabs only. Positive immunohistochemistry results, mainly observed as free bacteria or bacteria within inflammatory cell cytoplasm in the fibrinopurulent exudate, were observed in 8 of 10 animals. Cross-reactivity with Actinobacillus pleuropneumoniae was detected but not with other gram-positive and gram-negative bacteria tested. This immunohistochemistry technique seemed to be at least as sensitive as microbiologic cultures and could be useful in studies of pathogenesis and retrospective diagnosis. However, cross-reactivity with A. pleuropneumoniae means that positive immunohistochemistry results in lung tissue from field cases would be dubious.<\/jats:p>","DOI":"10.1177\/104063879700900303","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T16:18:45Z","timestamp":1305217125000},"page":"237-243","source":"Crossref","is-referenced-by-count":30,"title":["Immunohistochemical Detection of\n                    <i>Haemophilus Parasuis<\/i>\n                    Serovar 5 in Formalin-Fixed, Paraffin-Embedded Tissues of Experimentally Infected Swine"],"prefix":"10.1177","volume":"9","author":[{"given":"Joaquim","family":"Segal\u00e9s","sequence":"first","affiliation":[{"name":"U.D. Anatomia Patol\u00f2gica, Facultat de Veterinaria, Universitat Aut\u00f2noma de Barcelona, 08193 Bellaterra, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariano","family":"Domingo","sequence":"additional","affiliation":[{"name":"U.D. Anatomia Patol\u00f2gica, Facultat de Veterinaria, Universitat Aut\u00f2noma de Barcelona, 08193 Bellaterra, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gloria I.","family":"Solano","sequence":"additional","affiliation":[{"name":"Clinical and Population Sciences Department, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Pijoan","sequence":"additional","affiliation":[{"name":"Clinical and Population Sciences Department, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1997,7,1]]},"reference":[{"key":"bibr1-104063879700900303","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.56.639"},{"key":"bibr2-104063879700900303","doi-asserted-by":"publisher","DOI":"10.1177\/104063879100300418"},{"key":"bibr3-104063879700900303","first-page":"24","volume":"11","author":"McDaniels T","year":"1991","journal-title":"Agri-Practice"},{"key":"bibr4-104063879700900303","first-page":"187","volume":"21","author":"Menard J","year":"1990","journal-title":"Proc Annu Meet Am Assoc Swine Pract"},{"key":"bibr5-104063879700900303","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.15.6.1019-1023.1982"},{"key":"bibr6-104063879700900303","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.28.12.2711-2716.1990"},{"key":"bibr7-104063879700900303","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9975(08)80310-6"},{"key":"bibr8-104063879700900303","first-page":"526","volume-title":"Diseases of swine","author":"Nicolet J","year":"1992","edition":"7"},{"key":"bibr9-104063879700900303","doi-asserted-by":"publisher","DOI":"10.1186\/BF03548209"},{"key":"bibr10-104063879700900303","first-page":"20","volume":"27","author":"Nielsen R","year":"1975","journal-title":"Nord Veterin\u00e4rmed"},{"key":"bibr11-104063879700900303","doi-asserted-by":"publisher","DOI":"10.1139\/m84-195"},{"key":"bibr12-104063879700900303","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1983.tb05960.x"},{"key":"bibr13-104063879700900303","first-page":"335","volume":"12","author":"P\u00f6hle D","year":"1992","journal-title":"Proc Int Pig Vet Soc Cong"},{"key":"bibr14-104063879700900303","first-page":"945","volume":"22","author":"Rapp VJ","year":"1985","journal-title":"isolated from healthy swine and evaluation of cross-reactivity of complement fixing antibodies to Haemophilus pleuropneumoniae and Haemophilus taxon \u201cminor group.\u201d J Clin Microbiol"},{"key":"bibr15-104063879700900303","doi-asserted-by":"crossref","first-page":"659","DOI":"10.2460\/ajvr.1992.53.05.659","volume":"53","author":"Rapp-Gabrielson VJ","year":"1992","journal-title":"Am J Vet Res"},{"key":"bibr16-104063879700900303","first-page":"132","volume-title":"Pathogenesis of bacterial infections in animals","author":"Rosendal S","year":"1985","edition":"4"},{"key":"bibr17-104063879700900303","first-page":"487","volume":"34","author":"Smart NL","year":"1993","journal-title":"Can Vet J"},{"key":"bibr18-104063879700900303","first-page":"109","volume":"26","author":"White RD","year":"1995","journal-title":"Proc Am Assoc Swine Pract"},{"key":"bibr19-104063879700900303","doi-asserted-by":"crossref","first-page":"63","DOI":"10.2460\/ajvr.1996.57.01.63","volume":"57","author":"Zucker BA","year":"1996","journal-title":"Am J Vet Res"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063879700900303","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063879700900303","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:36:15Z","timestamp":1777682175000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/104063879700900303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,7]]},"references-count":19,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1997,7]]}},"alternative-id":["10.1177\/104063879700900303"],"URL":"https:\/\/doi.org\/10.1177\/104063879700900303","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,7]]}}}