{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T20:31:55Z","timestamp":1785529915660,"version":"3.56.0"},"reference-count":23,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[1997,10,1]],"date-time":"1997-10-01T00:00:00Z","timestamp":875664000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[1997,10]]},"abstract":"<jats:p>Specimens of blood, lymph nodes, spleens, and genitalia were collected at slaughter from seven 3- and 4-year-old male bison that had recently become seropositive for brucellosis. The animals were from a captive herd of approximately 3,500 bison located in central South Dakota. Brucella abortus biovar 1 was isolated from 2 or more specimens from each of 6 bison. Severe necrotizing and pyogranulomatous orchitis was present in 1 testicle from 1 bull, and 4 animals had mild to marked seminal vesiculitis. Immunohistochemical staining labeled organisms in seminal vesicles and the testicle with orchitis. Ultrastructurally, intact bacilli were present in cytoplasmic vacuoles of some macrophages; other macrophages contained intracytoplasmic aggregates of calcified coccobacilli.<\/jats:p>","DOI":"10.1177\/104063879700900405","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T16:18:45Z","timestamp":1305217125000},"page":"368-374","source":"Crossref","is-referenced-by-count":13,"title":["Seminal Vesiculitis and Orchitis Caused by\n                    <i>Brucella Abortus<\/i>\n                    Biovar 1 in Young Bison Bulls from South Dakota"],"prefix":"10.1177","volume":"9","author":[{"given":"Jack C.","family":"Rhyan","sequence":"first","affiliation":[{"name":"Pathobiology Laboratory, National Veterinary Services Laboratories, Veterinary Services, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, Ames, IA 50010"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sam D.","family":"Holland","sequence":"additional","affiliation":[{"name":"South Dakota Animal Industry Board, Pierre, SD 57501"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Gidlewski","sequence":"additional","affiliation":[{"name":"Diagnostic Bacteriology Laboratory, National Veterinary Services Laboratories, Veterinary Services, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, Ames, IA 50010"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis A.","family":"Saari","sequence":"additional","affiliation":[{"name":"Pathobiology Laboratory, National Veterinary Services Laboratories, Veterinary Services, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, Ames, IA 50010"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allen E.","family":"Jensen","sequence":"additional","affiliation":[{"name":"National Animal Disease Center, Agricultural Research Service, US Department of Agriculture, Ames, IA 50010"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darla R.","family":"Ewalt","sequence":"additional","affiliation":[{"name":"Diagnostic Bacteriology Laboratory, National Veterinary Services Laboratories, Veterinary Services, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, Ames, IA 50010"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve G.","family":"Hennager","sequence":"additional","affiliation":[{"name":"Diagnostic Bacteriology Laboratory, National Veterinary Services Laboratories, Veterinary Services, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, Ames, IA 50010"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven C.","family":"Olsen","sequence":"additional","affiliation":[{"name":"National Animal Disease Center, Agricultural Research Service, US Department of Agriculture, Ames, IA 50010"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norman F.","family":"Cheville","sequence":"additional","affiliation":[{"name":"Department of Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA 50010"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[1997,10,1]]},"reference":[{"key":"bibr1-104063879700900405","unstructured":"Alton GG, Jones LM, Angus RD, Verger JM: 1988, Techniques for the brucellosis laboratory. Institut National de la Recherche Agronomique, Paris, France. 190 pp"},{"key":"bibr2-104063879700900405","unstructured":"Anonymous: 1965a, Standard agglutination test procedures for the diagnosis of brucellosis. Diagnostic Reagents Manual 65D. USDA, APHIS, VS, NVSL, Ames, IA. 8 pp"},{"key":"bibr3-104063879700900405","unstructured":"Anonymous: 1965b, Supplemental test procedures for the diagnosis of brucellosis. Diagnostic Reagents Manual 65E. USDA, APHIS, VS, NVSL, Ames, IA. 23 pp"},{"key":"bibr4-104063879700900405","unstructured":"Anonymous: 1993,\n                      Brucella abortus\n                      complement fixation test micro method. 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