{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:23:50Z","timestamp":1791174230808,"version":"4.1.0"},"reference-count":29,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[1998,10,1]],"date-time":"1998-10-01T00:00:00Z","timestamp":907200000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[1998,10]]},"abstract":"<jats:p>In serology, lack of specificity can generally be attributed to cross-reactions between different pathogens with antigens bearing similar epitopes. During seroepidemiologic surveys of contagious agalactia of sheep caused by Mycoplasma agalactiae infection, numerous sera were analyzed by enzyme-linked immunosorbent assay (ELISA). A few sera reacted with various antigens coated on plates, including the well with no antigen. This reactivity was not due to cross-reactions as initially suspected, and these multipositive sera were designated false-positive sera. Elimination of this false positivity was not possible by using covalent ELISA plates or different rabbit anti-sheep IgG conjugates. Only conjugates using monoclonal antibodies or protein G were efficient in elimination of false positivities without reducing the true specific positive titers. No false-positive sera have been observed since the implementation of protein G conjugates in the serologic diagnosis of contagious agalactia by ELISA for the past 2 years.<\/jats:p>","DOI":"10.1177\/104063879801000403","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T16:18:45Z","timestamp":1305217125000},"page":"326-330","source":"Crossref","is-referenced-by-count":16,"title":["Detection of False-Positive Sera in Contagious Agalactia with a Multiantigen ELISA and their Elimination with a Protein G Conjugate"],"prefix":"10.1177","volume":"10","author":[{"given":"Maurice","family":"Lambert","sequence":"first","affiliation":[{"name":"CNEVA Sophia Antipolis, Unit\u00e9 Pathologie des Petits Ruminants, BP 111, F-06902 Sophia Antipolis cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Calamel","sequence":"additional","affiliation":[{"name":"CNEVA Sophia Antipolis, Unit\u00e9 Pathologie des Petits Ruminants, BP 111, F-06902 Sophia Antipolis cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Dufour","sequence":"additional","affiliation":[{"name":"CNEVA Sophia Antipolis, Unit\u00e9 Pathologie des Petits Ruminants, BP 111, F-06902 Sophia Antipolis cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelyne","family":"Cabasse","sequence":"additional","affiliation":[{"name":"CNEVA Sophia Antipolis, Unit\u00e9 Pathologie des Petits Ruminants, BP 111, F-06902 Sophia Antipolis cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Vitu","sequence":"additional","affiliation":[{"name":"CNEVA Sophia Antipolis, Unit\u00e9 Pathologie des Petits Ruminants, BP 111, F-06902 Sophia Antipolis cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"P\u00e9pin","sequence":"additional","affiliation":[{"name":"CNEVA Sophia Antipolis, Unit\u00e9 Pathologie des Petits Ruminants, BP 111, F-06902 Sophia Antipolis cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[1998,10,1]]},"reference":[{"key":"bibr1-104063879801000403","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)67515-5"},{"key":"bibr2-104063879801000403","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(89)90002-3"},{"key":"bibr3-104063879801000403","volume-title":"E.L.I.S.A. standardized technique.","author":"Calamel M","year":"1988"},{"key":"bibr4-104063879801000403","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(89)90041-2"},{"key":"bibr5-104063879801000403","doi-asserted-by":"publisher","DOI":"10.1177\/104063879200400126"},{"key":"bibr6-104063879801000403","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(93)90122-4"},{"key":"bibr7-104063879801000403","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.32.5.1159-1165.1994"},{"key":"bibr8-104063879801000403","doi-asserted-by":"publisher","DOI":"10.1136\/vr.137.6.145"},{"key":"bibr9-104063879801000403","first-page":"375","volume":"28","author":"Gerbier G","year":"1997","journal-title":"Vet Res"},{"key":"bibr10-104063879801000403","first-page":"186","volume":"17","author":"Hasselaar P","year":"1990","journal-title":"J Rheumatol"},{"key":"bibr11-104063879801000403","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(92)90034-B"},{"key":"bibr12-104063879801000403","doi-asserted-by":"publisher","DOI":"10.1016\/0147-9571(93)90159-3"},{"key":"bibr13-104063879801000403","doi-asserted-by":"publisher","DOI":"10.1080\/00480169.1996.35966"},{"key":"bibr14-104063879801000403","doi-asserted-by":"publisher","DOI":"10.20506\/rst.6.3.308"},{"key":"bibr15-104063879801000403","first-page":"107","volume":"140","author":"Lambert M","year":"1989","journal-title":"Rev M\u00e9d V\u00e9t"},{"key":"bibr16-104063879801000403","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.28.1.92-96.1990"},{"key":"bibr17-104063879801000403","doi-asserted-by":"crossref","first-page":"747","DOI":"10.2460\/javma.1989.195.06.747","volume":"195","author":"Lopez NA","year":"1989","journal-title":"J Am Vet Med Assoc"},{"key":"bibr18-104063879801000403","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(94)90088-4"},{"key":"bibr19-104063879801000403","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)90870-B"},{"key":"bibr20-104063879801000403","doi-asserted-by":"publisher","DOI":"10.1001\/jama.268.8.1015"},{"key":"bibr21-104063879801000403","doi-asserted-by":"publisher","DOI":"10.20506\/rst.12.2.712"},{"key":"bibr22-104063879801000403","doi-asserted-by":"publisher","DOI":"10.1080\/15321819208021232"},{"key":"bibr23-104063879801000403","doi-asserted-by":"crossref","first-page":"371","DOI":"10.19182\/remvt.8789","volume":"42","author":"Poumarat F","year":"1989","journal-title":"Rev Elev Med Vet Pays Trop"},{"key":"bibr24-104063879801000403","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(94)90057-4"},{"key":"bibr25-104063879801000403","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-40-4-293"},{"key":"bibr26-104063879801000403","doi-asserted-by":"publisher","DOI":"10.1177\/104063879200400410"},{"key":"bibr27-104063879801000403","doi-asserted-by":"crossref","first-page":"1550","DOI":"10.2460\/javma.1989.194.11.1550","volume":"194","author":"Tyler JW","year":"1989","journal-title":"J Am Vet Med Assoc"},{"key":"bibr28-104063879801000403","first-page":"35","volume":"38","author":"Yoshida H","year":"1992","journal-title":"Fukushima J Med Sci"},{"key":"bibr29-104063879801000403","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.27.3.580-582.1989"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063879801000403","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063879801000403","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:37:04Z","timestamp":1777682224000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/104063879801000403"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,10]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1998,10]]}},"alternative-id":["10.1177\/104063879801000403"],"URL":"https:\/\/doi.org\/10.1177\/104063879801000403","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,10]]}}}