{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T18:09:53Z","timestamp":1790964593874,"version":"4.1.0"},"reference-count":25,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[1999,3,1]],"date-time":"1999-03-01T00:00:00Z","timestamp":920246400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[1999,3]]},"abstract":"<jats:p>Genomic DNA from reference strains and biovars of the genus Brucella was analyzed using pulsed-field gel electrophoresis (PFGE). Fingerprints were compared to estimate genetic relatedness among the strains and to obtain information on evolutionary relationships. Electrophoresis of DNA digested with the restriction endonuclease XbaI produced fragment profiles for the reference type strains that distinguished these strains to the level of species. Included in this study were strains isolated from marine mammals. The PFGE profiles from these strains were compared with those obtained from the reference strains and biovars. Isolates from dolphins had similar profiles that were distinct from profiles of Brucella isolates from seals and porpoises. Distance matrix analyses were used to produce a dendrogram. Biovars of B. abortus were clustered together in the dendrogram; similar clusters were shown for biovars of B. melitensis and for biovars of B. suis. Brucella ovis, B. canis, and B. neotomae differed from each other and from B. abortus, B. melitensis, and B. suis. The relationship between B. abortus strain RB51 and other Brucella biovars was compared because this strain has replaced B. abortus strain 19 for use as a live vaccine in cattle and possibly in bison and elk. These results support the current taxonomy of Brucella species and the designation of an additional genomic group(s) of Brucella. The PFGE analysis in conjunction with distance matrix analysis was a useful tool for calculating genetic relatedness among the Brucella species.<\/jats:p>","DOI":"10.1177\/104063879901100208","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T16:20:37Z","timestamp":1305217237000},"page":"152-157","source":"Crossref","is-referenced-by-count":25,"title":["Genomic Fingerprinting and Development of a Dendrogram for\n                    <i>Brucella<\/i>\n                    spp. Isolated from Seals, Porpoises, and Dolphins"],"prefix":"10.1177","volume":"11","author":[{"given":"Allen E.","family":"Jensen","sequence":"first","affiliation":[{"name":"US Department of Agriculture, Agricultural Research Service, National Animal Disease Center, Zoonotic Diseases Research Unit, Ames, IA 50010"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norman F.","family":"Cheville","sequence":"additional","affiliation":[{"name":"Department of Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA 50011"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles O.","family":"Thoen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Preventative Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alastair P.","family":"MacMillan","sequence":"additional","affiliation":[{"name":"Central Veterinary Laboratory, OIE Brucellosis Reference Laboratory, FAO\/WHO Centre for Brucellosis Reference and Research, Weybridge, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. George","family":"Miller","sequence":"additional","affiliation":[{"name":"RDT&E Division of the Naval Command Control Ocean Surveillance Center, San Diego, CA 92152"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[1999,3,1]]},"reference":[{"key":"bibr1-104063879901100208","doi-asserted-by":"publisher","DOI":"10.1128\/jb.170.10.4603-4607.1988"},{"key":"bibr2-104063879901100208","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.7.2219-2224.1991"},{"key":"bibr3-104063879901100208","first-page":"9","volume-title":"Laboratory techniques in brucellosis","author":"Alton GG","year":"1967"},{"key":"bibr4-104063879901100208","first-page":"13","volume-title":"Techniques for the brucellosis laboratory","author":"Alton GG","year":"1988"},{"key":"bibr5-104063879901100208","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.32.11.2660-2666.1994"},{"key":"bibr6-104063879901100208","first-page":"377","volume-title":"Bergey's manual of systematic bacteriology","volume":"1","author":"Corbel MJ","year":"1984"},{"key":"bibr7-104063879901100208","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-37-1-35"},{"key":"bibr8-104063879901100208","doi-asserted-by":"publisher","DOI":"10.1177\/104063879400600408"},{"key":"bibr9-104063879901100208","doi-asserted-by":"publisher","DOI":"10.1136\/vr.138.24.583"},{"key":"bibr10-104063879901100208","doi-asserted-by":"publisher","DOI":"10.1177\/104063879700900311"},{"key":"bibr11-104063879901100208","first-page":"197","volume":"140","author":"Hall LMC","year":"1994","journal-title":"Microbiologia"},{"key":"bibr12-104063879901100208","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb01306.x"},{"key":"bibr13-104063879901100208","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.12.6637-6640.1990"},{"key":"bibr14-104063879901100208","doi-asserted-by":"publisher","DOI":"10.1128\/JB.95.2.444-448.1968"},{"key":"bibr15-104063879901100208","doi-asserted-by":"publisher","DOI":"10.1128\/JB.96.5.1783-1790.1968"},{"key":"bibr16-104063879901100208","doi-asserted-by":"crossref","first-page":"308","DOI":"10.2460\/ajvr.1995.56.03.308","volume":"56","author":"Jensen AE","year":"1995","journal-title":"Am J Vet Res"},{"key":"bibr17-104063879901100208","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.34.3.628-633.1996"},{"key":"bibr18-104063879901100208","first-page":"27","volume":"31","author":"Morgan WJB","year":"1976","journal-title":"Dev Biol Stand"},{"key":"bibr19-104063879901100208","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(85)90024-0"},{"key":"bibr20-104063879901100208","doi-asserted-by":"publisher","DOI":"10.1136\/vr.134.14.359-b"},{"key":"bibr21-104063879901100208","first-page":"6.1","volume-title":"Molecular cloning: a laboratory manual","author":"Sambrook J","year":"1989","edition":"2"},{"key":"bibr22-104063879901100208","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(84)90301-5"},{"key":"bibr23-104063879901100208","first-page":"145","volume":"13","author":"Stableforth AW","year":"1963","journal-title":"Int Bull Bacteriol Nomencl Taxon"},{"key":"bibr24-104063879901100208","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-35-3-292"},{"key":"bibr25-104063879901100208","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.11.3807-3811.1992"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063879901100208","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063879901100208","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:37:20Z","timestamp":1777682240000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/104063879901100208"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,3]]},"references-count":25,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1999,3]]}},"alternative-id":["10.1177\/104063879901100208"],"URL":"https:\/\/doi.org\/10.1177\/104063879901100208","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,3]]}}}