{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:52:07Z","timestamp":1777668727902,"version":"3.51.4"},"reference-count":45,"publisher":"SAGE Publications","issue":"7-8","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Human Gene Therapy"],"published-print":{"date-parts":[[2026,4,1]]},"abstract":"<jats:p>\n                    Recently, our research group generated induced tissue-specific stem\/progenitor (iTS\/iTP) cells. Compared with induced pluripotent stem (iPS) cells, iTS\/iTP cells offer several advantages, that is, easy generation, higher differentiation efficiency, and no teratoma formation. In this study, iTS cells were generated from mouse pancreatic tissues (iTS-P cells) using two different methods. Plasmid vectors were used for expressing\n                    <jats:italic toggle=\"yes\">Oct3\/4<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">Sox2<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">Klf4<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">c-Myc<\/jats:italic>\n                    (\n                    <jats:italic toggle=\"yes\">OSKM<\/jats:italic>\n                    ), or\n                    <jats:italic toggle=\"yes\">Yap1<\/jats:italic>\n                    (\n                    <jats:italic toggle=\"yes\">YAP<\/jats:italic>\n                    ) to evaluate the efficiency and differentiation potential of the resulting cells. No significant difference in reprogramming efficiency between the\n                    <jats:italic toggle=\"yes\">OSKM<\/jats:italic>\n                    - and\n                    <jats:italic toggle=\"yes\">YAP<\/jats:italic>\n                    -based methods was observed. Among the established clones, iTS-P OSKM2 and iTS-P YAP9 cells, which demonstrated high efficiency in differentiating to insulin-producing cells (IPCs), were selected for further comparison. Both iTS-P OSKM2 and iTS-P YAP9 cells expressed genetic markers of endoderm and pancreatic progenitors and differentiated into IPCs more efficiently than the embryonic stem (ES) cells. Genomic bisulfite sequencing revealed that the pluripotency factors\n                    <jats:italic toggle=\"yes\">Oct3\/4<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Nanog<\/jats:italic>\n                    were partially methylated in both iTS-P OSKM2 and iTS-P YAP9 cells. Unsupervised hierarchical clustering of gene expression profiles showed that iTS-P YAP9 cells clustered more closely with the ES cells than with the iTS-P OSKM2 cells. However, the expression levels of\n                    <jats:italic toggle=\"yes\">Oct3\/4<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Nanog<\/jats:italic>\n                    were significantly lower in both iTS-P OSKM2 and YAP9 cells than in the ES cells. These results conclude that no substantial difference is present in the characteristics between iTS-P cells induced by\n                    <jats:italic toggle=\"yes\">OSKM<\/jats:italic>\n                    or\n                    <jats:italic toggle=\"yes\">YAP<\/jats:italic>\n                    , and that their higher differentiation efficiency than the ES cells indicates promising potential for clinical applications.\n                  <\/jats:p>","DOI":"10.1177\/10430342251414882","type":"journal-article","created":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T08:11:11Z","timestamp":1770192671000},"page":"329-340","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Analysis of Induced Pancreatic Stem Cells Generated with Different Factors"],"prefix":"10.1177","volume":"37","author":[{"given":"Hirofumi","family":"Noguchi","sequence":"first","affiliation":[{"name":"Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chika","family":"Miyagi-Shiohira","sequence":"additional","affiliation":[{"name":"Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takuya","family":"Sadahira","sequence":"additional","affiliation":[{"name":"Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masami","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Issei","family":"Saitoh","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, Asahi University School of Dentistry, Hozumi, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2026,2,4]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.07.024"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.11.019"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1151526"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2007.05.014"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature05934"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature05944"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature06534"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1164270"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1172482"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1591"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.14.7999"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200203074"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.52.8.2007"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-006-0372-7"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1177\/096368970901805-612"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.3727\/215517913X666503"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.3727\/096368910X509004"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2014.132"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0163580"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-30784-0"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19040930"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtm.2019.01.011"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19113489"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.105052"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2022.101884"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2016.08.009"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.09.048"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00005.2014"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.06.013"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2016.05.005"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.1177\/09636897241248942"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1259"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1393"},{"key":"e_1_3_5_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2012.02.024"},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2011.407"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09404-6"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2506549"},{"key":"e_1_3_5_39_2","doi-asserted-by":"publisher","DOI":"10.1186\/s41232-019-0106-0"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09342"},{"key":"e_1_3_5_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1667"},{"key":"e_1_3_5_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.471"},{"key":"e_1_3_5_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09798"},{"key":"e_1_3_5_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2239"},{"key":"e_1_3_5_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2011.06.007"},{"key":"e_1_3_5_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2016.06.006"}],"container-title":["Human 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