{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T14:16:45Z","timestamp":1787926605422,"version":"build-2784847793"},"reference-count":23,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["DNA and Cell Biology"],"published-print":{"date-parts":[[2026,10,1]]},"abstract":"\n Mastitis caused by\n Staphylococcus aureus<\/jats:italic>\n is a common inflammatory disease in mammals. However, the role of carnitine palmitoyltransferase 1A (CPT1A) in this process remains poorly understood. In this study, we examined the expression and function of CPT1A using both\n in vitro<\/jats:italic>\n and\n in\u00a0vivo<\/jats:italic>\n models. HC11 cells were transfected with a CPT1A-overexpressing plasmid and then infected with\n S. aureus<\/jats:italic>\n , with or without the NOD-like receptor family pyrin domain containing 3 (NLRP3) agonist BMS-986299.\n In vivo<\/jats:italic>\n , we established a mouse mastitis model by injecting\n S. aureus<\/jats:italic>\n into the mammary duct and achieved local CPT1A overexpression via AAV9 delivery. The results showed that\n S. aureus<\/jats:italic>\n infection significantly downregulated CPT1A expression in mammary tissue and HC11 cells. CPT1A overexpression reversed the infection-induced decrease in cell viability, restored mitochondrial membrane potential, increased antioxidant enzyme activities, reduced malondialdehyde levels, and prevented the downregulation of Occludin and Claudin-3. Mechanistically, CPT1A overexpression was associated with enhanced NLRP3 succinylation and accelerated degradation, thereby inhibiting NLRP3 expression. Administration of the NLRP3 agonist BMS-986299 partially reversed the protective effects of CPT1A overexpression both\n in vitro<\/jats:italic>\n and\n in\u00a0vivo<\/jats:italic>\n . Collectively, these findings demonstrate that CPT1A counteracts\n S. aureus<\/jats:italic>\n -induced mastitis by promoting NLRP3 succinylation, which accelerates NLRP3 degradation, thereby alleviating mitochondrial oxidative stress and preserving blood\u2013milk barrier integrity. Targeting the CPT1A\u2013NLRP3 axis may represent a potential therapeutic strategy for\n S. aureus<\/jats:italic>\n -associated mastitis.\n <\/jats:p>","DOI":"10.1177\/10445498261473087","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T10:01:47Z","timestamp":1787133707000},"page":"509-521","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["CPT1A Ameliorates\n Staphylococcus aureus<\/i>\n -Induced Impairment of the Blood\u2013Milk Barrier and Mitochondrial Oxidative Stress in Mastitis by Promoting NLRP3 Succinylation"],"prefix":"10.1177","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-1261-8990","authenticated-orcid":false,"given":"Jie","family":"Xing","sequence":"first","affiliation":[{"name":"Thyroid Breast Surgery, Affiliated Hospital of Changchun University of Traditional Chinese Medicine, Changchun, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuzhuo","family":"Bai","sequence":"additional","affiliation":[{"name":"Thyroid Breast Surgery, Affiliated Hospital of Changchun University of Traditional Chinese Medicine, Changchun, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Deng","sequence":"additional","affiliation":[{"name":"Thyroid Breast Surgery, Affiliated Hospital of Changchun University of Traditional Chinese Medicine, Changchun, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Metabolic Diseases, and Spleen-Stomach Diseases, The Third Affiliated Hospital of Changchun University of Chinese Medicine, Changchun, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1011764"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-022-02135-1"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202303535"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/10641955.2025.2609374"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2018.07.016"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom14081011"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-025-02077-6"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2025.1577669"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2023.0004"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjpp-2020-0165"},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2021.105261"},{"key":"e_1_3_3_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M114.045575"},{"key":"e_1_3_3_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-022-04339-z"},{"key":"e_1_3_3_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202411947"},{"key":"e_1_3_3_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.760581"},{"key":"e_1_3_3_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-022-01027-y"},{"key":"e_1_3_3_18_1","first-page":"1171","article-title":"Magnetic resonance imaging of cardiovascular diseases: Advantages of coronal and sagittal planes]","volume":"15","author":"Watanabe S","year":"1985","unstructured":"Watanabe S, , Sakakibara M, , Imai H, et al. Magnetic resonance imaging of cardiovascular diseases: Advantages of coronal and sagittal planes]. J Cardiogr 1985;15:1171\u20131186.","journal-title":"J Cardiogr"},{"key":"e_1_3_3_19_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCHEARTFAILURE.124.012180"},{"key":"e_1_3_3_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.70322"},{"key":"e_1_3_3_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2023.10.043"},{"key":"e_1_3_3_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-025-02426-2"},{"key":"e_1_3_3_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41522-023-00374-8"},{"key":"e_1_3_3_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2024.113171"}],"container-title":["DNA and Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10445498261473087","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/10445498261473087","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10445498261473087","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T13:17:15Z","timestamp":1787923035000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/10445498261473087"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,19]]},"references-count":23,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10,1]]}},"alternative-id":["10.1177\/10445498261473087"],"URL":"https:\/\/doi.org\/10.1177\/10445498261473087","relation":{},"ISSN":["1044-5498","1557-7430"],"issn-type":[{"value":"1044-5498","type":"print"},{"value":"1557-7430","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,19]]}}}