{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T23:58:54Z","timestamp":1783641534015,"version":"3.55.0"},"reference-count":17,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[1993,4,1]],"date-time":"1993-04-01T00:00:00Z","timestamp":733622400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Critical Reviews in Oral Biology &amp; Medicine"],"published-print":{"date-parts":[[1993,4]]},"abstract":"<jats:p> The aim of the present study was to use an in vitro enamel demineralization model (1) to confirm that whole saliva pretreatment conferred acid resistance to dental enamel and (2) to determine whether this phenomenon was attributable to specific salivary proteins, minerals, lipids, or some combination of these. Crowns of human teeth, each with one exposed window, were prepared in groups of ten. They were each pretreated by immersion individually in 4 ml of either (1) clarified whole saliva for 18, 72, or 168 h, (2) dialyzed saliva (3500 MWCO membrane), (3) the \"flow-through\" fraction from a DEAE separation of whole saliva (neutral and basic proteins), (4) the \"eluted\" fraction of a DEAE separation of whole saliva (anionic proteins), or (5) a combination of salivary lipids and the DEAE \"flow-through\" fraction of whole saliva (neutral and basic proteins). Control groups were group 6 with no pretreatment, group 7 pretreated for 168 h in a borate buffer (5 mmol\/1), and group 8 pretreated in a mineral solution containing calcium (0.7 mmol\/1) and phosphate (2.6 mmol\/1). The crowns were then demineralized for 7 d in vitro (0.1 mol\/1 acetate, 1 mmol\/l Ca and phosphate, pH 5.0) to produce artificial caries-like lesions. Lesions were assessed by cross-sectional microhardness profiles, and mineral loss (AZ, \u03bcm x vol% mineral) calculated. Mineral loss (AZ) values decreased linearly with the square root of time of pretreatment by whole saliva, confirming a time-dependent protective effect of salivary pellicle against demineralization of enamel. Pretreatments (168 h) by whole saliva (group 1), dialyzed saliva (group 2), and lipid\/'flow through\" proteins (group 5) gave equivalent protection (approximately 55%). However, no protection was provided by DEAE-separated protein fractions (no lipid present) or by the mineral alone. The protection of surface enamel against demineralization appears to be given by a combination of specifically adsorbed salivary lipids and proteins. <\/jats:p>","DOI":"10.1177\/10454411930040031401","type":"journal-article","created":{"date-parts":[[2017,1,6]],"date-time":"2017-01-06T00:46:03Z","timestamp":1483663563000},"page":"357-362","source":"Crossref","is-referenced-by-count":31,"title":["Effect of Whole Saliva Components on Enamel Demineralization In Vitro"],"prefix":"10.1177","volume":"4","author":[{"given":"J.D.B.","family":"Featherstone","sequence":"first","affiliation":[{"name":"Department of Oral Sciences and Rochester Cariology Center, Eastman Dental Center, 625 Elmwood Avenue, Rochester, NY 14620"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.M.","family":"Behrman","sequence":"additional","affiliation":[{"name":"Department of Oral Sciences and Rochester Cariology Center, Eastman Dental Center, 625 Elmwood Avenue, Rochester, NY 14620"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.E.","family":"Bell","sequence":"additional","affiliation":[{"name":"Department of Oral Sciences and Rochester Cariology Center, Eastman Dental Center, 625 Elmwood Avenue, Rochester, NY 14620"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[1993,4,1]]},"reference":[{"key":"atypb1","doi-asserted-by":"publisher","DOI":"10.1159\/000259504"},{"key":"atypb2","unstructured":"Bertram, V.: Xerostomia: Clinical Aspects, Pathology and Pathogenesis, Acta Odont. Scand. (Suppl.) 49:31-124 (1967)."},{"key":"atypb3","doi-asserted-by":"crossref","unstructured":"Eggen, K. and G. Rolla: Further Information on the Composition of the Acquired Pellicle. Cariology Today, Int. Congr., Zurich, pp. 109-112 (1983).","DOI":"10.1159\/000408729"},{"key":"atypb4","doi-asserted-by":"publisher","DOI":"10.1159\/000260692"},{"key":"atypb5","doi-asserted-by":"crossref","unstructured":"Hay, D.I. and E.C. Moreno: Statherin and the Acidic Proline-Rich Proteins. In: Human Saliva: Clinical Chemistry and Microbiology, Vol. 1, pp. 131-150. ( J. O. Tenovuo, Ed.) CRC Press Inc., Boca Raton (1989).","DOI":"10.1201\/9781003210399-5"},{"key":"atypb6","doi-asserted-by":"publisher","DOI":"10.1177\/00220345920710090501"},{"key":"atypb7","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(75)90045-X"},{"key":"atypb8","doi-asserted-by":"publisher","DOI":"10.1177\/00220345740530021201"},{"key":"atypb9","unstructured":"Mandel, I.D.: Dental Caries. American Scientist 67:680-688 (1979)."},{"key":"atypb10","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(65)90004-X"},{"key":"atypb11","doi-asserted-by":"publisher","DOI":"10.1177\/10454411910020030101"},{"key":"atypb12","doi-asserted-by":"publisher","DOI":"10.1159\/000261026"},{"key":"atypb13","doi-asserted-by":"publisher","DOI":"10.1159\/000261059"},{"key":"atypb14","doi-asserted-by":"publisher","DOI":"10.1177\/00220345760550042101"},{"key":"atypb15","doi-asserted-by":"publisher","DOI":"10.1177\/00220345770560091601"},{"key":"atypb16","doi-asserted-by":"publisher","DOI":"10.1177\/00220345780570110601"},{"key":"atypb17","doi-asserted-by":"publisher","DOI":"10.1177\/00220345790580110501"}],"container-title":["Critical Reviews in Oral Biology &amp; Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10454411930040031401","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10454411930040031401","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,29]],"date-time":"2024-09-29T17:45:23Z","timestamp":1727631923000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/10454411930040031401"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,4]]},"references-count":17,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1993,4]]}},"alternative-id":["10.1177\/10454411930040031401"],"URL":"https:\/\/doi.org\/10.1177\/10454411930040031401","relation":{},"ISSN":["1045-4411","1544-1113"],"issn-type":[{"value":"1045-4411","type":"print"},{"value":"1544-1113","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,4]]}}}