{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T04:24:15Z","timestamp":1777523055566,"version":"3.51.4"},"reference-count":23,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2018,5,8]],"date-time":"2018-05-08T00:00:00Z","timestamp":1525737600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["The Cleft Palate Craniofacial Journal"],"published-print":{"date-parts":[[2019,3]]},"abstract":"\n Objective:<\/jats:title>\n \n Distal-less 4 (\n DLX4<\/jats:italic>\n ) was recently identified as the causative gene for a syndromic form of cleft lip with or without cleft palate, and further biological analyses have established the importance of\n Dlx4<\/jats:italic>\n gene in craniofacial development, which suggested\n DLX4<\/jats:italic>\n as a promising candidate to further investigate any possible association between\n DLX4<\/jats:italic>\n polymorphisms and risk to nonsyndromic orofacial clefts (NSOFCs).\n <\/jats:p>\n <\/jats:sec>\n \n Design:<\/jats:title>\n \n Single-nucleotide polymorphisms (SNPs) with minor allele frequency >5% in the Han Chinese population which locate in the 5\u2032 flanking region, 5\u2032\/3\u2032-untranslated region, or coding region with nonsynonymous changes in\n DLX4<\/jats:italic>\n were selected. Four SNPs (rs58769681, rs1058562, rs1058564, and rs8066341) were thus included in the following genotyping using the TaqMan 5\u2032-exonuclease allelic discrimination assay in a case\u2013control cohort with 1522 individuals.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n None of SNPs were associated with NSOFCat the allele and genotype levels in general and stratified single-marker analysis, including genotypic distributions under different modes of inheritance. In linkage disequilibrium (LD) analysis, we found strong LD (\n r<\/jats:italic>\n 2<\/jats:sup>\n > 0.8) between any 2 of the SNPs, respectively. Further haplotyping identified haplotypes C-C (formed by rs1058564 and rs1058562) and C-C-A (formed by rs1058564, rs1058562, and rs58769681) which reached the significance threshold (\n P<\/jats:italic>\n < .05); nevertheless, none of them survived the multiple comparison correction.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions:<\/jats:title>\n \n Our findings indicated the hypothesis that\n DLX4<\/jats:italic>\n variants contributing to NSOFC risk should be interpreted with caution. Further replications in diverse ethnic origins and larger cohorts are still warranted.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1177\/1055665618775723","type":"journal-article","created":{"date-parts":[[2018,5,8]],"date-time":"2018-05-08T16:37:44Z","timestamp":1525797464000},"page":"357-362","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Association Between\n DLX4<\/i>\n Polymorphisms and Nonsyndromic Orofacial Clefts in a Chinese Han Population"],"prefix":"10.1177","volume":"56","author":[{"given":"Miao","family":"He","sequence":"first","affiliation":[{"name":"State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuan","family":"Bian","sequence":"additional","affiliation":[{"name":"State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2018,5,8]]},"reference":[{"key":"e_1_3_2_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.580"},{"key":"e_1_3_2_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.333"},{"key":"e_1_3_2_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2350-14-53"},{"key":"e_1_3_2_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2933"},{"key":"e_1_3_2_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034509341040"},{"key":"e_1_3_2_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.23867"},{"key":"e_1_3_2_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034515581013"},{"key":"e_1_3_2_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.c.31381"},{"key":"e_1_3_2_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034514523987"},{"key":"e_1_3_2_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034514527617"},{"key":"e_1_3_2_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.506"},{"key":"e_1_3_2_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2011.07.007"},{"key":"e_1_3_2_14_1","doi-asserted-by":"publisher","DOI":"10.1086\/422475"},{"key":"e_1_3_2_15_1","doi-asserted-by":"publisher","DOI":"10.1159\/000353510"},{"key":"e_1_3_2_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddp444"},{"key":"e_1_3_2_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2009.01627.x"},{"key":"e_1_3_2_18_1","doi-asserted-by":"publisher","DOI":"10.1597\/10-178"},{"key":"e_1_3_2_19_1","doi-asserted-by":"publisher","DOI":"10.1086\/510518"},{"key":"e_1_3_2_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7414"},{"key":"e_1_3_2_21_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.049700"},{"key":"e_1_3_2_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004257"},{"key":"e_1_3_2_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddv167"},{"key":"e_1_3_2_24_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa032909"}],"container-title":["The Cleft Palate Craniofacial Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1055665618775723","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1055665618775723","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1055665618775723","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T16:19:30Z","timestamp":1777393170000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1055665618775723"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5,8]]},"references-count":23,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,3]]}},"alternative-id":["10.1177\/1055665618775723"],"URL":"https:\/\/doi.org\/10.1177\/1055665618775723","relation":{},"ISSN":["1055-6656","1545-1569"],"issn-type":[{"value":"1055-6656","type":"print"},{"value":"1545-1569","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,5,8]]}}}