{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T14:15:28Z","timestamp":1781014528576,"version":"3.54.1"},"reference-count":100,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2021,11,3]],"date-time":"2021-11-03T00:00:00Z","timestamp":1635897600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["DE018215"],"award-info":[{"award-number":["DE018215"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["HD97075"],"award-info":[{"award-number":["HD97075"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P20GM103453"],"award-info":[{"award-number":["P20GM103453"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["The Cleft Palate Craniofacial Journal"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Normal development of the embryonic orofacial region requires precise spatiotemporal coordination between numerous genes. MicroRNAs represent small, single-stranded, non-coding molecules that regulate gene expression. This study examines the role of microRNA-22 (miR-22) in murine orofacial ontogeny.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Spatiotemporal and differential expression of miR-22 (mmu-miR-22-3p) within the developing secondary palate was determined by in situ hybridization and quantitative real-time PCR, respectively. Bioinformatic approaches were used to predict potential mRNA targets of miR-22 and analyze their association with cellular functions indispensable for normal orofacial ontogeny. An in vitro palate organ culture system was used to assess the role of miR-22 in secondary palate development.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>There was a progressive increase in miR-22 expression from GD12.5 to GD14.5 in palatal processes. On GD12.5 and GD13.5, miR-22 was expressed in the future oral, nasal, and medial edge epithelia. On GD14.5, miR-22 expression was observed in the residual midline epithelial seam (MES), the nasal epithelium and the mesenchyme, but not in the oral epithelium. Inhibition of miR-22 activity in palate organ cultures resulted in failure of MES removal. Bioinformatic analyses revealed potential mRNA targets of miR-22 that may play significant roles in regulating apoptosis, migration, and\/or convergence\/extrusion, developmental processes that modulate MES removal during palatogenesis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Results from the current study suggest a key role for miR-22 in the removal of the MES during palatogenesis and that miR-22 may represent a potential contributor to the etiology of cleft palate.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/10556656211054004","type":"journal-article","created":{"date-parts":[[2021,11,3]],"date-time":"2021-11-03T10:56:50Z","timestamp":1635937010000},"page":"27-38","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Spatiotemporal Expression and Functional Analysis of miRNA-22 in the Developing Secondary Palate"],"prefix":"10.1177","volume":"60","author":[{"given":"Partha","family":"Mukhopadhyay","sequence":"first","affiliation":[{"name":"Department of Oral Immunology and Infectious Diseases, Division of Craniofacial Development and Anomalies, School of Dentistry, University of Louisville, Louisville, KY 40202"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Smolenkova","sequence":"additional","affiliation":[{"name":"Department of Oral Immunology and Infectious Diseases, Division of Craniofacial Development and Anomalies, School of Dentistry, University of Louisville, Louisville, KY 40202"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8925-1070","authenticated-orcid":false,"given":"Ratnam S.","family":"Seelan","sequence":"additional","affiliation":[{"name":"Department of Oral Immunology and Infectious Diseases, Division of Craniofacial Development and Anomalies, School of Dentistry, University of Louisville, Louisville, KY 40202"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Michele","family":"Pisano","sequence":"additional","affiliation":[{"name":"Department of Oral Immunology and Infectious Diseases, Division of Craniofacial Development and Anomalies, School of Dentistry, University of Louisville, Louisville, KY 40202"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6456-8103","authenticated-orcid":false,"given":"Robert M.","family":"Greene","sequence":"additional","affiliation":[{"name":"Department of Oral Immunology and Infectious Diseases, Division of Craniofacial Development and Anomalies, School of Dentistry, University of Louisville, Louisville, KY 40202"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,11,3]]},"reference":[{"key":"bibr1-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2019.10.001"},{"key":"bibr2-10556656211054004","doi-asserted-by":"crossref","first-page":"310","DOI":"10.4103\/0971-6866.107984","volume":"18","author":"Barritt LC","year":"2012","journal-title":"Indian J Hum Genet"},{"key":"bibr3-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.01.002"},{"key":"bibr4-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.03.006"},{"key":"bibr5-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.2008.00604.x"},{"key":"bibr6-10556656211054004","first-page":"345","volume":"39","author":"Brunet CL","year":"1995","journal-title":"Int J Dev Biol"},{"key":"bibr7-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1242\/dev.067082"},{"key":"bibr8-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1242\/dev.114.2.379"},{"key":"bibr9-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20821"},{"key":"bibr10-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81732-8"},{"key":"bibr11-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1038\/ejhg.2008.149"},{"key":"bibr12-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2006.12.002"},{"key":"bibr13-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00907"},{"key":"bibr14-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2002.0620"},{"key":"bibr15-10556656211054004","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2017.4942"},{"key":"bibr16-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2016.27"},{"key":"bibr17-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2003.12.003"},{"key":"bibr18-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.04.005"},{"key":"bibr19-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-02854-7"},{"key":"bibr20-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ctdb.2015.07.009"},{"key":"bibr21-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1002\/bdrc.20180"},{"key":"bibr22-10556656211054004","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2017.00039"},{"key":"bibr23-10556656211054004","doi-asserted-by":"publisher","DOI":"10.3349\/ymj.2020.61.5.371"},{"key":"bibr24-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.12565"},{"key":"bibr25-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awz406"},{"key":"bibr26-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1159\/000337619"},{"key":"bibr27-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2010.12.021"},{"key":"bibr28-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2010.08.014"},{"key":"bibr29-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7580.2005.00474.x"},{"key":"bibr30-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1002\/jez.b.21274"},{"key":"bibr31-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1177\/0022034511408613"},{"key":"bibr32-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-169X.2012.01364.x"},{"key":"bibr33-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1002\/bdr2.1768"},{"key":"bibr34-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2009.0935"},{"key":"bibr35-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02520"},{"key":"bibr36-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.24177"},{"key":"bibr37-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1038\/ng1295-415"},{"key":"bibr38-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx1141"},{"key":"bibr39-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1002122"},{"key":"bibr40-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1159\/000320178"},{"key":"bibr41-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth843"},{"key":"bibr42-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0059463"},{"key":"bibr43-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ctdb.2015.07.002"},{"key":"bibr44-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2005.10.007"},{"key":"bibr45-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1177\/0022034517703580"},{"key":"bibr46-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2010.10.023"},{"key":"bibr47-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2009.07920.x"},{"key":"bibr48-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b18-00084"},{"key":"bibr49-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/5845402"},{"key":"bibr50-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.29061"},{"key":"bibr51-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.498634"},{"key":"bibr52-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(99)00118-1"},{"key":"bibr53-10556656211054004","first-page":"152","volume":"92","author":"Ma HL","year":"2015","journal-title":"Biol Reprod"},{"key":"bibr54-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1002\/bdr2.1589"},{"key":"bibr55-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ctdb.2015.09.001"},{"key":"bibr56-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.2006.047837"},{"key":"bibr57-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.9644"},{"key":"bibr58-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1242\/dev.148007"},{"key":"bibr59-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1111\/jop.12950"},{"key":"bibr60-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1002\/bdra.20684"},{"key":"bibr61-10556656211054004","doi-asserted-by":"publisher","DOI":"10.2174\/2211536607666180801094528"},{"key":"bibr62-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2010.12.002"},{"key":"bibr63-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2015.06.014"},{"key":"bibr64-10556656211054004","doi-asserted-by":"publisher","DOI":"10.3171\/2016.8.SPINE16248"},{"key":"bibr65-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1002\/humu.23415"},{"key":"bibr66-10556656211054004","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.5636"},{"key":"bibr67-10556656211054004","doi-asserted-by":"publisher","DOI":"10.4317\/medoral.18357"},{"key":"bibr68-10556656211054004","doi-asserted-by":"crossref","first-page":"e0132662","DOI":"10.1371\/journal.pone.0132662","volume":"10","author":"Potter AS","year":"2015","journal-title":"PLoS One"},{"key":"bibr69-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1038\/ng1295-409"},{"key":"bibr70-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1177\/0022034517716915"},{"key":"bibr71-10556656211054004","doi-asserted-by":"crossref","first-page":"e0139359","DOI":"10.1371\/journal.pone.0139359","volume":"10","author":"Rustagi Y","year":"2015","journal-title":"PLoS One"},{"key":"bibr72-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2016.08.019"},{"key":"bibr74-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.22546"},{"key":"bibr73-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2005.07.027"},{"key":"bibr75-10556656211054004","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2017.00165"},{"key":"bibr76-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2013.07.029"},{"key":"bibr77-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1242\/dev.052647"},{"key":"bibr78-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.10.079"},{"key":"bibr79-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-012-0915-6"},{"key":"bibr80-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2012.07.004"},{"key":"bibr81-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2018.02.003"},{"key":"bibr82-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2013.06.027"},{"key":"bibr83-10556656211054004","unstructured":"TarBase. TarBase v.8. 2021. Available at: http:\/\/carolina.imis.athena-innovation.gr\/diana_tools\/web\/index.php?r=tarbasev8%2Findex (accessed June 10, 2021)."},{"key":"bibr84-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.17.3869"},{"key":"bibr85-10556656211054004","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1111\/j.1600-0722.2012.00967.x","volume":"120","author":"Vukojevic K","year":"2012","journal-title":"Eur J Oral Sci"},{"key":"bibr86-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2018.03.006"},{"key":"bibr87-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1007\/s10529-012-1018-0"},{"key":"bibr88-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/j.livres.2020.06.001"},{"key":"bibr89-10556656211054004","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2017.4248"},{"key":"bibr90-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1556\/ABiol.62.2011.2.4"},{"key":"bibr91-10556656211054004","doi-asserted-by":"publisher","DOI":"10.2174\/2211536604666150710125743"},{"key":"bibr92-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.082578dw"},{"key":"bibr93-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.35594"},{"key":"bibr94-10556656211054004","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.16742"},{"key":"bibr95-10556656211054004","doi-asserted-by":"publisher","DOI":"10.2147\/CMAR.S185124"},{"key":"bibr96-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.05.014"},{"key":"bibr97-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1242\/dev.052001"},{"key":"bibr98-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1242\/dev.083352"},{"key":"bibr99-10556656211054004","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1005253"},{"key":"bibr100-10556656211054004","first-page":"7209","volume":"17","author":"Zhou X","year":"2018","journal-title":"Mol Med Rep"}],"container-title":["The Cleft Palate Craniofacial Journal"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10556656211054004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/10556656211054004","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10556656211054004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T16:21:28Z","timestamp":1777393288000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.sagepub.com\/doi\/10.1177\/10556656211054004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,3]]},"references-count":100,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1177\/10556656211054004"],"URL":"https:\/\/doi.org\/10.1177\/10556656211054004","relation":{},"ISSN":["1055-6656","1545-1569"],"issn-type":[{"value":"1055-6656","type":"print"},{"value":"1545-1569","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,3]]}}}