{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T12:14:39Z","timestamp":1783426479465,"version":"3.54.6"},"reference-count":33,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T00:00:00Z","timestamp":1757289600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Guizhou Provincial Science and Technology Program","award":["Qian Ke He Basic Research - ZK[2022] General Progr"],"award-info":[{"award-number":["Qian Ke He Basic Research - ZK[2022] General Progr"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["Grant No. 82360183"],"award-info":[{"award-number":["Grant No. 82360183"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["The Cleft Palate Craniofacial Journal"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To investigate the effects of zinc concentration on palatal development in fetal mice and its association with the aryl hydrocarbon receptor (AhR) signaling pathway.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Pregnant C57BL\/6J mice were fed diets with varying zinc concentrations and randomly divided into a zinc-rich (ZR) group, a normal-zinc (NZ) group, and a zinc-deficient (ZD) group. Embryonic development was observed, and the expression levels of AhR signaling pathway-related factors were examined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      No cleft palate was observed in the ZR group or NZ group, whereas the zinc-deficient group exhibited a cleft palate incidence of 27.45%. Hematoxylin and Eosin (HE) staining results revealed failed palatal shelf contact and fusion in the ZD group, whereas complete fusion occurred in the ZR group and a normal medial edge epithelial formed in the NZ group. Results from Immunohistochemistry (IHC), qRT-PCR, and Western blot analyses collectively demonstrated that, compared to the NZ group, the ZR group exhibited significant upregulation (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      \u2009&lt;\u2009.05) in both mRNA and protein expression levels of AhR, HSP90, CYP1A1, and SP1, whereas the ZD group displayed significant downregulation (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      \u2009&lt;\u2009.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Zinc concentration is intimately correlated with fetal mouse palatal development, where zinc deficiency may contribute to cleft palate formation through suppression of AhR signaling pathway, whereas zinc-rich conditions facilitate activation of this pathway. This study reveals the regulatory role of zinc-AhR signaling axis in palatal development through mouse models, offering novel theoretical insights into zinc deficiency-induced cleft palate pathogenesis and establishing a foundational framework for preventive interventions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/10556656251375952","type":"journal-article","created":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T14:22:33Z","timestamp":1757341353000},"page":"2339-2347","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["A Preliminary Study of the Relationship Between Zinc and the AhR Signaling Pathway During Palatal Development"],"prefix":"10.1177","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-8530-2181","authenticated-orcid":false,"given":"Shenhu","family":"Liang","sequence":"first","affiliation":[{"name":"Zunyi Medical University"},{"name":"People's Hospital of Zhongjiang County, Deyang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxiang","family":"Zhai","sequence":"additional","affiliation":[{"name":"Zunyi Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongwei","family":"Chen","sequence":"additional","affiliation":[{"name":"Zunyi Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Yang","sequence":"additional","affiliation":[{"name":"Zunyi Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Shu","sequence":"additional","affiliation":[{"name":"Zunyi Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianmin","family":"Luo","sequence":"additional","affiliation":[{"name":"Zunyi Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Wang","sequence":"additional","affiliation":[{"name":"Zunyi Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Wang","sequence":"additional","affiliation":[{"name":"Zunyi Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5944-9879","authenticated-orcid":false,"given":"Qinggao","family":"Song","sequence":"additional","affiliation":[{"name":"Zunyi Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,9,8]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.656865"},{"issue":"7","key":"e_1_3_2_3_2","article-title":"Global birth prevalence of orofacial clefts: A systematic review[J]","volume":"98","author":"Panamonta V","year":"2015","unstructured":"Panamonta V, Pradubwong S, Panamonta M, Chowchuen B. 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