{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T10:19:25Z","timestamp":1777717165475,"version":"3.51.4"},"reference-count":43,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2016,11,30]],"date-time":"2016-11-30T00:00:00Z","timestamp":1480464000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Surg Pathol"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:p>Background. Diagnostic confirmation of spindle-cell melanoma (SM) or desmoplastic melanoma (DM) as a melanoma can be challenging. In conventional melanoma (CM), a recently established fluorescence in situ hybridization (FISH) assay for RREB1, MYB, CCND1 can be helpful. Here, we determined the presence of RREB1, MYB, and CCND1 abnormalities in an SM\/DM\/mixed cohort. Methods. We assembled 49 cases and performed 3 separate hybridizations for RREB1\/MYB\/CCND1. We assessed clinical utility in diagnostically challenging cases and performed a cost and turnaround time analysis. Results. With regard to the diagnosis of melanoma, the FISH assay is 76% sensitive (n = 31\/41 true positives melanomas) and 88% specific (n = 1\/8 false positive desmoplastic nevi). The prevalence of abnormalities in DM is lower (12\/19 cases, 63%; P = .03) than in SM (15\/18 cases, 83%; P = .27), mixed (4 of 4 cases), or the reported sensitivity in CM (345\/411 cases, 84%). The implied genetic differences in DM result in a higher false negative rate in DM (37%). Despite these limitations, when restricted to diagnostically challenging cases (n = 23), the FISH assay and, in particular, RREB1 was able to confirm melanoma in 70% (n = 16\/23). Individual probe sensitivities ( RREB1 &gt; MYB &gt; CCND1) and a cost and turnaround time analysis argues for a 2-step test algorithm that reduces the economic impact of FISH testing considerably (~55%; n = 69 vs 123 hybridizations). Conclusion. We propose a step-by-step genetic testing algorithm to support the diagnosis of melanoma in the setting of SM\/DM and show that FISH testing is useful in diagnostically challenging cases.<\/jats:p>","DOI":"10.1177\/1066896916680072","type":"journal-article","created":{"date-parts":[[2016,11,30]],"date-time":"2016-11-30T00:03:06Z","timestamp":1480464186000},"page":"148-157","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Performance Testing of\n                    <i>RREB1<\/i>\n                    ,\n                    <i>MYB<\/i>\n                    , and\n                    <i>CCND1<\/i>\n                    Fluorescence In Situ Hybridization in Spindle-Cell and Desmoplastic Melanoma Argues for a Two-Step Test Algorithm"],"prefix":"10.1177","volume":"25","author":[{"given":"Stephanie E.","family":"Weissinger","sequence":"first","affiliation":[{"name":"University of Ulm, Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manfred","family":"Frick","sequence":"additional","affiliation":[{"name":"University of Ulm, Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"M\u00f6ller","sequence":"additional","affiliation":[{"name":"University of Ulm, Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Basil A.","family":"Horst","sequence":"additional","affiliation":[{"name":"Columbia University Medical Center, New York, NY, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jochen K.","family":"Lennerz","sequence":"additional","affiliation":[{"name":"University of Ulm, Ulm, Germany"},{"name":"Massachusetts General Hospital\/Harvard Medical, Boston, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2016,11,30]]},"reference":[{"key":"bibr1-1066896916680072","first-page":"76","volume-title":"World Health Organization Classification of Tumors Pathology and Genetics of Skin Tumours","author":"McCarthy SW","year":"2006"},{"key":"bibr2-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1080\/00313020412331285336"},{"key":"bibr3-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1111\/ajd.12121"},{"key":"bibr4-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2009.075226"},{"key":"bibr5-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2010.075440"},{"key":"bibr6-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0560.2010.01568.x"},{"key":"bibr7-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1245\/ASO.2005.03.022"},{"key":"bibr8-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2011.196"},{"key":"bibr9-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2013.162"},{"key":"bibr10-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2004.23600.x"},{"key":"bibr11-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa050092"},{"key":"bibr12-1066896916680072","first-page":"2170","volume":"58","author":"Bastian BC","year":"1998","journal-title":"Cancer Res"},{"key":"bibr13-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e3181a1ef36"},{"key":"bibr14-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1080\/00313020902915875"},{"key":"bibr15-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2009.72"},{"key":"bibr16-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1001\/archdermatol.2009.386"},{"key":"bibr17-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2010.228"},{"key":"bibr18-1066896916680072","doi-asserted-by":"crossref","first-page":"830","DOI":"10.5858\/2011-0048-RAIR.1","volume":"135","author":"Gerami P","year":"2011","journal-title":"Arch Pathol Lab Med"},{"key":"bibr19-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1097\/PAP.0b013e3182169b69"},{"key":"bibr20-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2011.03984.x"},{"key":"bibr21-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1177\/1066896912445923"},{"key":"bibr22-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2013.04.001"},{"key":"bibr23-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0560.2010.01666.x"},{"key":"bibr24-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e318229cf66"},{"key":"bibr25-1066896916680072","first-page":"169","volume":"101","author":"Clemente C","year":"2009","journal-title":"Pathologica"},{"key":"bibr26-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2013.128"},{"key":"bibr27-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-199612000-00008"},{"key":"bibr28-1066896916680072","doi-asserted-by":"crossref","first-page":"327","DOI":"10.5858\/2009-0733-RA.1","volume":"135","author":"Zembowicz A","year":"2011","journal-title":"Arch Pathol Lab Med"},{"key":"bibr29-1066896916680072","doi-asserted-by":"publisher","DOI":"10.2353\/jmoldx.2006.050083"},{"key":"bibr30-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2014.59"},{"key":"bibr31-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1097\/PAI.0b013e318279bc0a"},{"key":"bibr32-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1097\/DAD.0b013e31827a0b98"},{"key":"bibr33-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2230.2012.04416.x"},{"key":"bibr34-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2010.03607.x"},{"key":"bibr35-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e3181c805c4"},{"key":"bibr36-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1111\/pde.12382"},{"key":"bibr37-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2009.177"},{"key":"bibr38-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2012.04293.x"},{"key":"bibr39-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2015.39"},{"key":"bibr40-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2011.04087.x"},{"key":"bibr41-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2010.05.043"},{"key":"bibr42-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0560.2011.01675.x"},{"key":"bibr43-1066896916680072","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2016.04.059"}],"container-title":["International Journal of Surgical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1066896916680072","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1066896916680072","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1066896916680072","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T10:21:26Z","timestamp":1777458086000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1066896916680072"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,30]]},"references-count":43,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,4]]}},"alternative-id":["10.1177\/1066896916680072"],"URL":"https:\/\/doi.org\/10.1177\/1066896916680072","relation":{},"ISSN":["1066-8969","1940-2465"],"issn-type":[{"value":"1066-8969","type":"print"},{"value":"1940-2465","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,30]]}}}