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A 53-year-old woman with post-menopausal vaginal bleeding underwent surgical excision followed by concurrent chemoradiation. A well demarcated tumor was growing in a discontinuous fashion at a location some distance from both the cervix and vulva. Microscopically, the tumor consisted of adenocarcinoma components and sarcoma components consisting of a sheet-like growth of spindle-shaped cells, and we diagnosed this tumor as primary vaginal carcinosarcoma. NGS analysis of each component identified the following variants, TP53, PIK3CA, KRAS and FBXW7. A comparison of microsatellite instability (MSI) and tumor mutation burden (TMB) showed that within both tissues the sarcomatous components had a higher MSI and TMB than the carcinomatous components. This case supports \u201ca monoclonal theory\u201d with the genome profile being similar to other malignant mixed M\u00fcllerian tumors.<\/jats:p>","DOI":"10.1177\/10668969211037915","type":"journal-article","created":{"date-parts":[[2021,8,31]],"date-time":"2021-08-31T06:23:10Z","timestamp":1630390990000},"page":"288-294","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Two Components of Variant Profiles in Primary Vaginal Carcinosarcoma via Next-Generation Sequencing and a Literature Review"],"prefix":"10.1177","volume":"30","author":[{"given":"Rakan","family":"Kotoku","sequence":"first","affiliation":[{"name":"Department of Obstetrics & Gynecology, Faculty of Medicine, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima 890-8520, 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