{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T22:44:32Z","timestamp":1780353872228,"version":"3.54.1"},"reference-count":14,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2022,2,14]],"date-time":"2022-02-14T00:00:00Z","timestamp":1644796800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Surg Pathol"],"published-print":{"date-parts":[[2022,9]]},"abstract":"Soft tissue tumors with RAF1 fusion had been emerging as a group of tumors with peculiar histology and immunoprofile. While a case series and rare case reports of RAF1 translocated sarcoma had been reported, to our knowledge a primary bone tumor with RAF1 translocation and fusion partner with MAP4 had not been described in the literature. The patient was a 60-year-old lady, with strong family history of breast cancer, who presented with pathological fracture of right humerus. X-ray revealed a 9.7\u2005cm juxta-articular lesion of the proximal humerus, which was expansile and lytic with a non-sclerotic well defined border distally, radiologically suggestive of a giant cell tumor of bone. Excision was performed after initial biopsy. Histology showed a monomorphic low grade spindle cell lesion with prominent hyalinized stroma. Immunohistochemistry demonstrated diffuse CD34 staining, with focal staining for S100. Gene sequencing for histone 3 H3 genes was negative for hotspot mutation. Targeted RNA-seq sequencing revealed the presence of MAP4::RAF1 fusion, which was confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR) and fluorescence in-situ hybridization (FISH) break-apart probes involving both genes. The overall features were consistent with a primary bone sarcoma with RAF1 fusion. This report expanded the spectrum of RAF1 fusion sarcoma and was the first report documenting its primary occurrence in bone.<\/jats:p>","DOI":"10.1177\/10668969221080059","type":"journal-article","created":{"date-parts":[[2022,2,14]],"date-time":"2022-02-14T11:52:33Z","timestamp":1644839553000},"page":"682-688","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":10,"title":["Novel\n MAP4::RAF1<\/i>\n Fusion in a Primary Bone Sarcoma: Expanding the spectrum of\n RAF1<\/i>\n Fusion Sarcoma"],"prefix":"10.1177","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9942-0109","authenticated-orcid":false,"given":"Maximus C. F.","family":"Yeung","sequence":"first","affiliation":[{"name":"Department of Pathology, The University of Hong Kong, Queen Mary Hospital, HKSAR"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert Y. L.","family":"Lam","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics and Traumatology, The University of Hong Kong, Queen Mary Hospital, HKSAR"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tony W. H.","family":"Shek","sequence":"additional","affiliation":[{"name":"Department of Pathology, The University of Hong Kong, Queen Mary Hospital, HKSAR"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,2,14]]},"reference":[{"key":"bibr1-10668969221080059","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22846"},{"key":"bibr2-10668969221080059","doi-asserted-by":"publisher","DOI":"10.1186\/s13000-018-0759-z"},{"key":"bibr3-10668969221080059","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22671"},{"key":"bibr4-10668969221080059","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-021-00806-w"},{"key":"bibr5-10668969221080059","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000675"},{"key":"bibr6-10668969221080059","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-020-00657-x"},{"key":"bibr7-10668969221080059","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"},{"key":"bibr8-10668969221080059","doi-asserted-by":"publisher","DOI":"10.1101\/120295"},{"key":"bibr9-10668969221080059","doi-asserted-by":"publisher","DOI":"10.1101\/gr.257246.119"},{"key":"bibr10-10668969221080059","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-020-0510-7"},{"key":"bibr11-10668969221080059","doi-asserted-by":"publisher","DOI":"10.1126\/science.1260419"},{"key":"bibr12-10668969221080059","doi-asserted-by":"publisher","DOI":"10.1148\/rg.331125089"},{"key":"bibr13-10668969221080059","doi-asserted-by":"publisher","DOI":"10.1007\/s00292-019-0623-2"},{"key":"bibr14-10668969221080059","doi-asserted-by":"publisher","DOI":"10.1038\/nm0710-749"}],"container-title":["International Journal of Surgical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10668969221080059","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/10668969221080059","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10668969221080059","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T10:22:19Z","timestamp":1777458139000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/10668969221080059"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,14]]},"references-count":14,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,9]]}},"alternative-id":["10.1177\/10668969221080059"],"URL":"https:\/\/doi.org\/10.1177\/10668969221080059","relation":{},"ISSN":["1066-8969","1940-2465"],"issn-type":[{"value":"1066-8969","type":"print"},{"value":"1940-2465","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,14]]}}}