{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T23:01:12Z","timestamp":1793228472661,"version":"4.5.0"},"reference-count":11,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2022,3,27]],"date-time":"2022-03-27T00:00:00Z","timestamp":1648339200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Surg Pathol"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:p>Introduction: Weak acids, such as etheylenediaminetetraacetic acid (EDTA), chelate calcium ions from the surface of tissues, decalcifying slowly but preserving molecular structures for ancillary testing. There is a need for optimization of EDTA protocols for specimen decalcification. We studied the effects of EDTA on different types of bony specimens for quality assurance. Methods: Specimens included: proximal femur curettage (0.7\u2005g), fibula shave (1\u2005mm thick, 0.5\u2005g), tibia shave (2\u2005mm thick, 0.9\u2005g), and femur 11-gauge core biopsies. Curettage and shave specimens were placed in formalin then EDTA (Newcomersupply, Middleton, WI, USA) with continuous stirring. Specimens were removed at 24, 48, 72, 96, and 120\u2005hours. Core biopsies were processed in EDTA with heat at 4, 8, 12, and 16\u2005hours. X-ray imaging (Kubtec Xpert 40, Stratford, CT, USA) was obtained. Histological processing was attempted and H&amp;E slides compared to radiologic imaging. Results: Fibula and tibia sections showed appropriate radiolucency at 120\u2005hours and 7 days, respectively. Curettage specimens showed radiolucency of medullary bone at 48\u2005hours. Curettage and fibula sections showed quality histology at 96\u2005hours and 120\u2005hours, respectively. Quality tibia sections were obtained at 7 days, requiring one hour of additional block surface decalcification with 1% HCL solution. Medullary and cortical core biopsies showed quality histology at 12 and 16\u2005hours with heat, respectively. Conclusion: Imaging can determine appropriate decalcification of bony specimens. Medullary bone undergoes more rapid decalcification in EDTA than cortical bone. EDTA decalcification is best used for curettage and core biopsy specimens.<\/jats:p>","DOI":"10.1177\/10668969221088877","type":"journal-article","created":{"date-parts":[[2022,3,28]],"date-time":"2022-03-28T02:54:25Z","timestamp":1648436065000},"page":"853-860","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["A Quality Analysis of Bony Specimens for Optimal Ethylenediaminetetraacetic Acid (EDTA) Decalcification"],"prefix":"10.1177","volume":"30","author":[{"given":"J. Cody","family":"Craig","sequence":"first","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Freeman","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Schaundra","family":"Walton","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Demonica","family":"Rogers","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitra","family":"Mehrad","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0745-2160","authenticated-orcid":false,"given":"Jennifer B.","family":"Gordetsky","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA"},{"name":"Department of Urology, Vanderbilt University Medical Center, Nashville, TN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,3,27]]},"reference":[{"key":"bibr1-10668969221088877","volume-title":"Histotechnology: A Self-Instructional Text","author":"Carson FL","year":"2002"},{"key":"bibr2-10668969221088877","doi-asserted-by":"publisher","DOI":"10.1369\/0022155417733708"},{"key":"bibr3-10668969221088877","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-020-0503-6"},{"key":"bibr4-10668969221088877","doi-asserted-by":"publisher","DOI":"10.1016\/j.anndiagpath.2013.02.001"},{"key":"bibr5-10668969221088877","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0257416"},{"key":"bibr6-10668969221088877","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2016.116"},{"key":"bibr7-10668969221088877","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2021.08.007"},{"key":"bibr8-10668969221088877","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000001321"},{"key":"bibr9-10668969221088877","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-0989-7_15"},{"key":"bibr10-10668969221088877","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqz161"},{"key":"bibr11-10668969221088877","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-020-02848-y"}],"container-title":["International Journal of Surgical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10668969221088877","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/10668969221088877","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10668969221088877","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T10:22:26Z","timestamp":1777458146000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/10668969221088877"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,27]]},"references-count":11,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["10.1177\/10668969221088877"],"URL":"https:\/\/doi.org\/10.1177\/10668969221088877","relation":{},"ISSN":["1066-8969","1940-2465"],"issn-type":[{"value":"1066-8969","type":"print"},{"value":"1940-2465","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,27]]}}}