{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T08:28:07Z","timestamp":1787646487271,"version":"build-2736575974"},"reference-count":22,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T00:00:00Z","timestamp":1700697600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Surg Pathol"],"published-print":{"date-parts":[[2024,9]]},"abstract":"\n Loss of histone H3K27 Trimethylation (H3K27me3) immunohistochemical expression is commonly used as an ancillary test and a surrogate marker for the diagnosis of malignant peripheral nerve sheath tumor (MPNST). A potential histological mimic of MPNST is sarcomatoid carcinoma. Prompted by an index specimen of sarcomatoid carcinoma with H3K27me3 loss and the lack of literature on such phenomenon, we sought to determine the frequency of H3K27me3 loss of expression in a cohort of sarcomatoid carcinomas. Fifty specimens of primary and metastatic sarcomatoid carcinomas with spindle cell morphology mimicking MPNST were prospectively and retrospectively retrieved from our institutional archives and stained with an antibody to H3K27me3. H3K27me3 staining was lost in 4 of the 50 specimens (8%). These specimens included a primary sarcomatoid urothelial carcinoma of the bladder resection, two local recurrences (sarcomatoid squamous cell carcinoma of the larynx and oral cavity) as well as a metastatic sarcomatoid renal cell carcinoma. Next-generation sequencing performed on all four specimens demonstrated gene mutations and copy number alterations with\n TP53<\/jats:italic>\n ,\n FANC<\/jats:italic>\n (\n FANCD2<\/jats:italic>\n and\n FANCI<\/jats:italic>\n ), and\n TERT<\/jats:italic>\n being the most common gene mutations and\n CDKN2A\/B<\/jats:italic>\n copy number loss and 11q region amplification being the most common copy number gene alterations. Mutations involving\n NF1, SUZ12,<\/jats:italic>\n or\n EED<\/jats:italic>\n were absent in all tested specimens. In conclusion, H3K27me3 expression may be lost in as many as 8% of sarcomatoid carcinomas which can pose as a potential diagnostic pitfall, especially in challenging sarcomatoid carcinoma specimens with absent keratin staining.\n <\/jats:p>","DOI":"10.1177\/10668969231215819","type":"journal-article","created":{"date-parts":[[2023,11,24]],"date-time":"2023-11-24T01:40:11Z","timestamp":1700790011000},"page":"1089-1094","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Loss of Histone H3K27 Trimethylation (H3K27me3) Expression as a Potential Diagnostic Pitfall in Sarcomatoid Carcinoma"],"prefix":"10.1177","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2473-4474","authenticated-orcid":false,"given":"Megan L.","family":"Zilla","sequence":"first","affiliation":[{"name":"University of Pittsburgh Medical Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4751-7678","authenticated-orcid":false,"given":"Ivy","family":"John","sequence":"additional","affiliation":[{"name":"University of Pittsburgh Medical Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5556-7376","authenticated-orcid":false,"given":"Rana","family":"Naous","sequence":"additional","affiliation":[{"name":"University of Pittsburgh Medical Center"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,11,23]]},"reference":[{"key":"e_1_3_3_2_2","unstructured":"Nielsen GP Chi P. Malignant Peripheral Nerve Sheath Tumor. WHO Classification of Tumours Editorial Board. Soft Tissue and Bone Tumours. Lyon (France): International Agency for Research on Cancer; 2020. (WHO classification of tumours series 5th ed.; vol. 3). https:\/\/publications.iarc.fr\/588."},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2015.134"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000564"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqx060"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3095"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-17-0151"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1260200"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-021-00776-z"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2016.1790"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.5301\/jbm.5000168"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/his.13703"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40246-015-0054-y"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks638"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.07.003"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/371257a0"},{"key":"e_1_3_3_17_2","article-title":"TERT Promoter mutations are enriched in oral cavity cancers and associated with locoregional recurrence","volume":"5","author":"Yu Y","year":"2021","unstructured":"Yu Y, Fan D, Song X, et al. TERT Promoter mutations are enriched in oral cavity cancers and associated with locoregional recurrence. JCO Precis Oncol. 2021;5:PO.20.00515.","journal-title":"JCO Precis Oncol"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1481"},{"key":"e_1_3_3_19_2","article-title":"P53 mutations in human cancers","author":"Hollstein M","unstructured":"Hollstein M, Sidransky D, Vogelstein B, Harris CC. P53 mutations in human cancers. Science. 1991;253(5015):49-53.","journal-title":"Science"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/his.14223"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3095"},{"issue":"1","key":"e_1_3_3_22_2","article-title":"Genetics of human malignant peripheral nerve sheath tumors","volume":"2","author":"Pemov A","year":"2019","unstructured":"Pemov A, Li H, Presley W, Wallace MR, Miller DT. Genetics of human malignant peripheral nerve sheath tumors. Neurooncol Adv. 2019;2(Suppl 1):i50-i61.","journal-title":"Neurooncol Adv"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-016-1540-6"}],"container-title":["International Journal of Surgical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10668969231215819","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/10668969231215819","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10668969231215819","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T08:12:02Z","timestamp":1787645522000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/10668969231215819"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,23]]},"references-count":22,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1177\/10668969231215819"],"URL":"https:\/\/doi.org\/10.1177\/10668969231215819","relation":{},"ISSN":["1066-8969","1940-2465"],"issn-type":[{"value":"1066-8969","type":"print"},{"value":"1940-2465","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,23]]}}}