{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T08:28:26Z","timestamp":1787646506906,"version":"build-2736575974"},"reference-count":38,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T00:00:00Z","timestamp":1726444800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Surg Pathol"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:p>\n                    Epidermal growth factor receptor (\n                    <jats:italic toggle=\"yes\">EGFR<\/jats:italic>\n                    ) mutation screening in non-small cell lung cancer (NSCLC) is now used to guide treatment decisions to identify patients with\n                    <jats:italic toggle=\"yes\">EGFR<\/jats:italic>\n                    positive mutations that predict response to\n                    <jats:italic toggle=\"yes\">EGFR<\/jats:italic>\n                    tyrosine kinase inhibitors. This study aimed to explore with a prospective study the current testing practices and the predictive value of\n                    <jats:italic toggle=\"yes\">EGFR<\/jats:italic>\n                    mutations in a series of 261 patients with NSCLC. EGFR mutation testing was conducted using 2 different assays: bidirectional Sanger sequencing of polymerase chain reaction (PCR) and real-time PCR on the Rotor-Gene Q instrument. Epidermal growth factor receptor mutation testing was performed for 261 patients with lung cancer. Exons 18 to 21 were successfully analyzed in 113 tumors by Direct sequencing and in 148 tumors by real-time PCR. The prevalence of positive\n                    <jats:italic toggle=\"yes\">EGFR<\/jats:italic>\n                    -mutations in each method was 22.1% (N\u2009=\u200925) and 24.3% (N\u2009=\u200936), respectively (\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    \u2009=\u2009.3). In total,\n                    <jats:italic toggle=\"yes\">EGFR<\/jats:italic>\n                    mutations were detected in 59 patients among 261 patients with NSCLC. A statistically significant association between female sex, nonsmoking history, nonsolid major pattern, and a higher\n                    <jats:italic toggle=\"yes\">EGFR<\/jats:italic>\n                    mutation frequency. In this study, we investigated clinicopathological differences between tumors harboring exon 19del and those harboring L858R. We did not find any significant differences between the 2 mutations and gender or smoking features, interestingly, the prevalence of patients aged &gt;60 years was significantly higher in the L858R group than in the exon 19del group (81.8% vs 55.8%,\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    \u2009=\u2009.05). A significant association was observed between exon 19 deletions and the papillary major pattern, but no correlation was detected between exon 21 mutation and any histological pattern. This prospective study documented the real-world clinical testing of\n                    <jats:italic toggle=\"yes\">EGFR<\/jats:italic>\n                    mutation in Moroccan NSCLC patients. Our experience confirms the need to develop standards-based guidelines for the routine performance and evaluation of\n                    <jats:italic toggle=\"yes\">EGFR<\/jats:italic>\n                    testing to improve clinical care for this subset of lung cancer. On the other hand, our study demonstrated that tumors with exon 19 deletions and L858R harbor specific clinicopathological features in NSCLC.\n                  <\/jats:p>","DOI":"10.1177\/10668969241268379","type":"journal-article","created":{"date-parts":[[2024,9,17]],"date-time":"2024-09-17T14:52:46Z","timestamp":1726584766000},"page":"585-595","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Testing for\n                    <i>EGFR<\/i>\n                    Mutation in Moroccan NSCLC Patients: CHU Hassan II-Fez Experience"],"prefix":"10.1177","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4297-3380","authenticated-orcid":false,"given":"Sara","family":"Boukansa","sequence":"first","affiliation":[{"name":"Sidi Mohamed Ben Abdellah University"},{"name":"Sidi Mohamed Ben Abdellah University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ismail","family":"Mouhrach","sequence":"additional","affiliation":[{"name":"Sidi Mohamed Ben Abdellah University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4467-4555","authenticated-orcid":false,"given":"Fatima El","family":"Agy","sequence":"additional","affiliation":[{"name":"Sidi Mohamed Ben Abdellah University"},{"name":"Sidi Mohamed Ben Abdellah University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanaa","family":"Gamrani","sequence":"additional","affiliation":[{"name":"Sidi Mohamed Ben Abdellah University"},{"name":"Sidi Mohamed Ben Abdellah University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laila","family":"Bouguenouch","sequence":"additional","affiliation":[{"name":"Sidi Mohamed Ben Abdellah University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mounia","family":"Serraj","sequence":"additional","affiliation":[{"name":"Sidi Mohamed Ben Abdellah University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bouchra","family":"Amara","sequence":"additional","affiliation":[{"name":"Sidi Mohamed Ben Abdellah University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yassine","family":"Ouadnouni","sequence":"additional","affiliation":[{"name":"Sidi Mohamed Ben Abdellah University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Smahi","sequence":"additional","affiliation":[{"name":"Sidi Mohamed Ben Abdellah University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Badreeddine","family":"Alami","sequence":"additional","affiliation":[{"name":"Sidi Mohamed Ben Abdellah University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nawfel","family":"Mellas","sequence":"additional","affiliation":[{"name":"Sidi Mohamed Ben Abdellah University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zineb","family":"Benbrahim","sequence":"additional","affiliation":[{"name":"Sidi Mohamed Ben Abdellah University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hinde El","family":"Fatemi","sequence":"additional","affiliation":[{"name":"Sidi Mohamed Ben Abdellah University"},{"name":"Sidi Mohamed Ben Abdellah University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,9,16]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0033-1342949"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2011.37.2185"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa040938"},{"issue":"1","key":"e_1_3_2_5_2","first-page":"36","article-title":"Targeted therapy for non-small cell lung cancer: current standards and the promise of the future","volume":"4","author":"Chan BA","year":"2015","unstructured":"Chan BA, Hughes BGM. Targeted therapy for non-small cell lung cancer: current standards and the promise of the future. Transl Lung Cancer Res. 2015;4(1):36. doi:https:\/\/doi.org\/10.3978\/J.ISSN.2218-6751.2014.05.01","journal-title":"Transl Lung Cancer Res"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-021-01121-2"},{"issue":"1","key":"e_1_3_2_7_2","first-page":"48","article-title":"Epidermal growth factor receptor (EGFR) in lung cancer: an overview and update","volume":"2","author":"Bethune G","year":"2010","unstructured":"Bethune G, Bethune D, Ridgway N, Xu Z. Epidermal growth factor receptor (EGFR) in lung cancer: an overview and update. J Thorac Dis. 2010;2(1):48\u201351.","journal-title":"J Thorac Dis"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers9050052"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0143515"},{"issue":"9","key":"e_1_3_2_10_2","first-page":"2892","article-title":"EGFR mutation incidence in non-small-cell lung cancer of adenocarcinoma histology: a systematic review and global map by ethnicity (mutMapII)","volume":"5","author":"Midha A","year":"2015","unstructured":"Midha A, Dearden S, McCormack R. EGFR mutation incidence in non-small-cell lung cancer of adenocarcinoma histology: a systematic review and global map by ethnicity (mutMapII). 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Oncogene. 2009;28 Suppl 1(Suppl 1):S24hyphen;S31. doi:https:\/\/doi.org\/10.1038\/ONC.2009.198","journal-title":"Oncogene"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1177\/17588359221146131"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0043842"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2012-201240"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1097\/JTO.0000000000000481"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.29812"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1097\/JTO.0b013e3182769aa8"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0076164"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1556-0864(15)31573-2"},{"issue":"6","key":"e_1_3_2_25_2","first-page":"3189","article-title":"Relationship between EGFR mutations and clinicopathological features of lung adenocarcinomas diagnosed via small biopsies - PubMed","volume":"34","author":"Kim HJ","year":"2014","unstructured":"Kim HJ, Choi EY, Jin HJ, Shin K-C. 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