{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T09:18:32Z","timestamp":1787649512577,"version":"build-2736575974"},"reference-count":37,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T00:00:00Z","timestamp":1762992000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100003806","name":"Hokuto Foundation for Bioscience","doi-asserted-by":"publisher","award":["none"],"award-info":[{"award-number":["none"]}],"id":[{"id":"10.13039\/501100003806","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Surg Pathol"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:p>\n                    Hepatocellular carcinoma (HCC) is a leading cause of cancer-related deaths. The role of leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5), a cancer stem cell marker, in HCC remains unclear. This study evaluated\n                    <jats:italic toggle=\"yes\">LGR5<\/jats:italic>\n                    expression in 50 primary solitary HCC patients using RNAscope, a highly sensitive RNA in situ hybridization method, and assessed its clinicopathological and prognostic significance. The\n                    <jats:italic toggle=\"yes\">LGR5<\/jats:italic>\n                    was expressed in some normal hepatocytes, particularly smaller cells, suggesting its involvement in regeneration. In HCC,\n                    <jats:italic toggle=\"yes\">LGR5<\/jats:italic>\n                    expression was detected in 25 patients. High\n                    <jats:italic toggle=\"yes\">LGR5<\/jats:italic>\n                    expression in the tumor center was significantly associated with high-grade HCC (\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    \u2009=\u2009.0491). At the invasion front, high\n                    <jats:italic toggle=\"yes\">LGR5<\/jats:italic>\n                    expression was correlated with high-grade HCC (\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    \u2009=\u2009.0140) and larger tumor size (&gt;5 cm) (\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    \u2009=\u2009.0334). A Kaplan-Meier analysis showed no significant difference in overall survival (OS) between the groups. However, high\n                    <jats:italic toggle=\"yes\">LGR5<\/jats:italic>\n                    expression at the tumor center was linked to shorter recurrence-free survival (RFS) (\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    \u2009=\u2009.0449). On multivariate analysis, high\n                    <jats:italic toggle=\"yes\">LGR5<\/jats:italic>\n                    expression at the tumor center was significantly associated with shorter RFS (HR, 6.77; 95% CI, 1.51-30.31;\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    \u2009=\u2009.0124) and larger tumor size (&gt;5 cm) (HR, 6.36; 95% CI, 1.57-25.84;\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    \u2009=\u2009.0096). The\n                    <jats:italic toggle=\"yes\">LGR5<\/jats:italic>\n                    expression at the invasion front was not significantly associated with either OS or RFS. The present findings suggest that\n                    <jats:italic toggle=\"yes\">LGR5<\/jats:italic>\n                    expression is associated with aggressive HCC characteristics and increased risk of recurrence, highlighting its potential as a prognostic marker for RFS in high-grade HCC.\n                  <\/jats:p>","DOI":"10.1177\/10668969251384894","type":"journal-article","created":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T22:03:52Z","timestamp":1763071432000},"page":"407-415","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>LGR5<\/i>\n                    Expression Is Associated With Prognosis in High-Grade Hepatocellular Carcinoma"],"prefix":"10.1177","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-1726-5881","authenticated-orcid":false,"given":"Shinsuke","family":"Sugenoya","sequence":"first","affiliation":[{"name":"Shinshu University School of 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