{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T20:57:18Z","timestamp":1790024238278,"version":"4.0.1"},"reference-count":31,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cancer Control"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>The association between the expression of HIF-1\u03b1 in the laryngeal carcinoma and the prognosis of disease is quite well documented, but the significance of HIF-1\u03b1 C1772T polymorphism and its relation to disease phenotype have to be clarified. The aim of this study was to investigate the influence of C1772T polymorphism on the clinical-pathological characteristics and disease-free survival after initial surgical treatment of patients with laryngeal carcinoma.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>The prospective cohort study included 65 patients with laryngeal carcinoma. Two representative tumor tissue specimens were taken in each patient during surgery; 1 specimen was used to asses HIF-1\u03b1 C1772T polymorphism and the other 1 to determine the immunohistochemical expression of HIF-1\u03b1, VEGF, as well as CD 34 proteins. The comparison of polymorphism frequency between study and control population was conducted by collecting a 5\u00a0mL of peripheral venous blood samples in each subject.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Clinicopathological characteristics of laryngeal carcinoma didn\u2019t affect the expression of hypoxia-related biomarkers, such as HIF-1\u03b1, VEGF or MVD. The statistically significant association between HIF-1\u03b1 and VEGF expression was found ( P = .034), but not between HIF-1\u03b1 expression and MVD value ( P = .696). The expression of HIF-1\u03b1 was significantly higher among CT heterozygotes ( P = .029). We found a significantly more recurrence among CT heterozygotes compared with patients with CC homozygous alleles (57.10% and 24.30%, respectively; P = .007). Patients with C1772T polymorphic variants had significantly worse disease-free survival compared with patients without polymorphism (Log-rank test, P = .007).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>HIF-1\u03b1 C1772T polymorphism was significantly associated with worse disease-free survival which nominates it as a predictor of laryngeal carcinoma relapse. The preoperative assessment of hypoxia-related biomarkers should be used in everyday practice in order to determine the treatment modalities for laryngeal carcinoma.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/10732748221144457","type":"journal-article","created":{"date-parts":[[2022,12,5]],"date-time":"2022-12-05T17:56:43Z","timestamp":1670263003000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["The Expression of Hypoxia-Related Biomarkers: A Significance of HIF-1\u03b1 C1772T Polymorphism as Predictor of Laryngeal Carcinoma Relapse"],"prefix":"10.1177","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6049-0342","authenticated-orcid":false,"given":"Miljan M.","family":"Folic","sequence":"first","affiliation":[{"name":"Faculty of Medicine, University of Belgrade, Belgrade, Serbia"},{"name":"Clinic for Otorhinolaryngology and Maxillofacial Surgery, University Clinical Centre of Serbia, Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana V.","family":"Banko","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Belgrade, Belgrade, Serbia"},{"name":"Institute of Microbiology and Immunology, Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera N.","family":"Todorovic","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, School of Medicine of University of Zenica, Zenica, Bosnia and Herzegovina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nela S.","family":"Puskas","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Belgrade, Belgrade, Serbia"},{"name":"Institute of Histology and Embryology \u201cProf. Dr Aleksandar Dj. Kostic\u201d, Faculty of Medicine, University of Belgrade, Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jovica P.","family":"Milovanovic","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Belgrade, Belgrade, Serbia"},{"name":"Clinic for Otorhinolaryngology and Maxillofacial Surgery, University Clinical Centre of Serbia, Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanja B.","family":"Krejovic","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Belgrade, Belgrade, Serbia"},{"name":"Clinic for Otorhinolaryngology and Maxillofacial Surgery, University Clinical Centre of Serbia, Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neda Z.","family":"Dragicevic-Babic","sequence":"additional","affiliation":[{"name":"Center for Rare Diseases \u2013 Reference Center Northern Bavaria (ZESE), University Hospital Wuerzburg, Wurzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoran M.","family":"Bukumiric","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Belgrade, Belgrade, Serbia"},{"name":"Department of Medical Statistics and Informatics, Faculty of Medicine, University of Belgrade, Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biljana R.","family":"Milicic","sequence":"additional","affiliation":[{"name":"Statistics and Informatics, School of Dental Medicine, University of Belgrade, Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana D.","family":"Jotic","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Belgrade, Belgrade, Serbia"},{"name":"Clinic for Otorhinolaryngology and Maxillofacial Surgery, University Clinical Centre of Serbia, Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vojko B.","family":"Djukic","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Belgrade, Belgrade, Serbia"},{"name":"Clinic for Otorhinolaryngology and Maxillofacial Surgery, University Clinical Centre of Serbia, Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,12,5]]},"reference":[{"key":"bibr1-10732748221144457","doi-asserted-by":"publisher","DOI":"10.21147\/j.issn.1000-9604.2020.01.03"},{"key":"bibr2-10732748221144457","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21492"},{"key":"bibr3-10732748221144457","unstructured":"National Cancer Institute. 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