{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T17:45:21Z","timestamp":1777484721789,"version":"3.51.4"},"reference-count":27,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cancer Control"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To determine the dysregulated signaling pathways of head and neck squamous cell carcinoma associated with circulating tumor cells (CTCs) via single-cell molecular characterization.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Head and neck squamous cell carcinoma (HNSCC) has a significant global burden and is a disease with poor survival. Despite trials exploring new treatment modalities to improve disease control rates, the 5\u00a0year survival rate remains low at only 60%. Most cancer malignancies are reported to progress to a fatal phase due to the metastatic activity derived from treatment-resistant cancer cells, regarded as one of the most significant obstacles to develope effective cancer treatment options. However, the molecular profiles of cancer cells have not been thoroughly studied.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Here, we examined in-situ HNSCC tumors and pairwisely followed up with the downstream circulating tumor cells (CTCs)-based on the surrogate biomarkers to detect metastasis that is established in other cancers \u2013 not yet being fully adopted in HNSCC treatment algorithms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Specifically, we revealed metastatic HNSCC patients have complex CTCs that could be defined through gene expression and mutational gene profiling derived from completed single-cell RNASeq (scRNASeq) that served to confirm molecular pathways inherent in these CTCs. To enhance the reliability of our findings, we cross-validated those molecular profiles with results from previously published studies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Thus, we identified 5 dysregulated signaling pathways in CTCs to derive HNSCC biomarker panels for screening HNSCC in situ tumors.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/10732748241251571","type":"journal-article","created":{"date-parts":[[2024,6,13]],"date-time":"2024-06-13T06:27:58Z","timestamp":1718260078000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":8,"title":["Single-Cell Molecular Profiling of Head and Neck Squamous Cell Carcinoma Reveals Five Dysregulated Signaling Pathways Associated With Circulating Tumor Cells"],"prefix":"10.1177","volume":"31","author":[{"given":"Andres","family":"Stucky","sequence":"first","affiliation":[{"name":"Department of Basic Sciences, School of Medicine, Loma Linda University, Loma Linda, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chi T.","family":"Viet","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, School of Dentistry, Loma Linda University, Loma Linda, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bradley E.","family":"Aouizerat","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, College of Dentistry, New York University, New York, NY, USA"},{"name":"Bluestone Center for Clinical Research, College of Dentistry, New York University, New York, NY, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, College of Dentistry, New York University, New York, NY, USA"},{"name":"Bluestone Center for Clinical Research, College of Dentistry, New York University, New York, NY, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Coleen","family":"Doan","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, School of Dentistry, Loma Linda University, Loma Linda, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tarun","family":"Mundluru","sequence":"additional","affiliation":[{"name":"Herman Ostrow School of Dentistry, Department of Periodontics and Diagnostic Sciences, University of Southern California, Los Angeles, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parish","family":"Sedhiazadeh","sequence":"additional","affiliation":[{"name":"Herman Ostrow School of Dentistry, Department of Periodontics and Diagnostic Sciences, University of Southern California, Los Angeles, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uttam K.","family":"Sinha","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuelian","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences, School of Medicine, Loma Linda University, Loma Linda, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences, School of Medicine, Loma Linda University, Loma Linda, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9699-9204","authenticated-orcid":false,"given":"Shengwen Calvin","family":"Li","sequence":"additional","affiliation":[{"name":"Neuro-Oncology and Stem Cell Research Laboratory, Center for Neuroscience Research, CHOC Children\u2019s Research Institute, Children\u2019s Hospital of Orange County, Orange, CA, USA"},{"name":"Department of Neurology, University of California\u2014Irvine School of Medicine, Orange, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, Zhuhai People\u2019s Hospital, Zhuhai Clinical Medical College of Jinan University, Zhuhai, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiang F.","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences, School of Medicine, Loma Linda University, Loma Linda, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2024,6,13]]},"reference":[{"key":"bibr1-10732748241251571","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.29189"},{"key":"bibr2-10732748241251571","doi-asserted-by":"publisher","DOI":"10.1002\/hed.24589"},{"key":"bibr3-10732748241251571","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.12086"},{"issue":"1","key":"bibr4-10732748241251571","first-page":"116","volume":"3","author":"Li SC","year":"2018","journal-title":"Stem Cell Res Ther"},{"key":"bibr5-10732748241251571","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2018.01.064"},{"key":"bibr6-10732748241251571","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870140209"},{"key":"bibr7-10732748241251571","doi-asserted-by":"publisher","DOI":"10.1002\/1878-0261.13113"},{"key":"bibr8-10732748241251571","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-016-2468-x"},{"key":"bibr9-10732748241251571","doi-asserted-by":"publisher","DOI":"10.1002\/ohn.186"},{"key":"bibr10-10732748241251571","doi-asserted-by":"publisher","DOI":"10.4252\/wjsc.v12.i8.706"},{"key":"bibr11-10732748241251571","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2023.216187"},{"key":"bibr12-10732748241251571","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.8b01272"},{"key":"bibr13-10732748241251571","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4096"},{"key":"bibr14-10732748241251571","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/giy083"},{"key":"bibr15-10732748241251571","volume-title":"A Language and Environment for Statistical Computing","author":"R: A language and environment for statistical computing Secondary R","year":"2018"},{"key":"bibr16-10732748241251571","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-03056-x"},{"key":"bibr17-10732748241251571","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1135489"},{"key":"bibr18-10732748241251571","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.39304.486285.DB"},{"key":"bibr19-10732748241251571","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.31638"},{"key":"bibr20-10732748241251571","doi-asserted-by":"publisher","DOI":"10.1016\/j.neo.2022.100799"},{"key":"bibr21-10732748241251571","doi-asserted-by":"publisher","DOI":"10.3390\/ph15030378"},{"key":"bibr22-10732748241251571","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2022.105720"},{"key":"bibr23-10732748241251571","doi-asserted-by":"publisher","DOI":"10.1016\/j.tranon.2021.101117"},{"key":"bibr24-10732748241251571","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2022.105383"},{"key":"bibr25-10732748241251571","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8101504"},{"key":"bibr26-10732748241251571","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-022-10256-5"},{"key":"bibr27-10732748241251571","doi-asserted-by":"publisher","DOI":"10.4252\/wjsc.v10.i2.15"}],"container-title":["Cancer Control"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10732748241251571","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/10732748241251571","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10732748241251571","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T14:24:01Z","timestamp":1777386241000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/10732748241251571"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1]]},"references-count":27,"alternative-id":["10.1177\/10732748241251571"],"URL":"https:\/\/doi.org\/10.1177\/10732748241251571","relation":{},"ISSN":["1073-2748","1526-2359"],"issn-type":[{"value":"1073-2748","type":"print"},{"value":"1526-2359","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1]]},"article-number":"10732748241251571"}}