{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T06:07:29Z","timestamp":1777529249410,"version":"3.51.4"},"reference-count":29,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2010,2,4]],"date-time":"2010-02-04T00:00:00Z","timestamp":1265241600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cardiovasc Pharmacol Ther"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:p>\n                    Hydrogen sulfide (H\n                    <jats:sub>2<\/jats:sub>\n                    S) is a biological mediator produced by enzyme-regulated pathways from L-cysteine, which is a substrate for cystathionine-\u03b3-lyase (CSE). In myocardium, endogenously and exogenously administered H\n                    <jats:sub>2<\/jats:sub>\n                    S has been shown to protect against ischemia-reperfusion injury. We hypothesized that L-cysteine exerts its protective action through stimulation of H\n                    <jats:sub>2<\/jats:sub>\n                    S production. Rat isolated hearts were Langendorff-perfused and underwent 35-minute regional ischemia and 120-minute reperfusion. L-cysteine perfusion from 10 minutes before ischemia until 10 minutes after reperfusion limited infarct size in a concentration-dependent manner, maximal at 1 mmol\/L (control 36.4% \u00b1 2.4% vs L-cysteine 24.3% \u00b1 3.4%, P &lt; .05). This protective action was attenuated by the CSE inhibitor, DL-propargylglycine (PAG) 1 mmol\/L (31.4 \u00b1 5.9%, not significant vs control) but administration of the CSE cofactor pyridoxal-5\u2032-phosphate (PLP) 50 \u03bcmol\/L did not enhance the effect of L-cysteine. Ten minutes normoxic perfusion with L-cysteine 1 mmol\/L caused a 3-fold increase in myocardial H\n                    <jats:sub>2<\/jats:sub>\n                    S concentration (0.64 \u00b1 0.16 vs 2.01 \u00b1 0.07 \u03bcmol\/g protein, P &lt; .01), an effect that was significantly attenuated by PAG (1.17 \u00b1 0.15 \u03bcmol\/g protein). These data provide evidence that exogenous L-cysteine administration limits ischemia-reperfusion injury through a mechanism that appears to be at least partially dependent on H\n                    <jats:sub>2<\/jats:sub>\n                    S synthesis.\n                  <\/jats:p>","DOI":"10.1177\/1074248409357743","type":"journal-article","created":{"date-parts":[[2010,2,4]],"date-time":"2010-02-04T19:19:04Z","timestamp":1265311144000},"page":"53-59","source":"Crossref","is-referenced-by-count":33,"title":["L-Cysteine Stimulates Hydrogen Sulfide Synthesis in Myocardium Associated With Attenuation of Ischemia-Reperfusion Injury"],"prefix":"10.1177","volume":"15","author":[{"given":"David J.","family":"Elsey","sequence":"first","affiliation":[{"name":"Welsh School of Pharmacy, Cardiff University, Cardiff, United Kingdom, Royal Veterinary College, University of London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert C.","family":"Fowkes","sequence":"additional","affiliation":[{"name":"Royal Veterinary College, University of London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary F.","family":"Baxter","sequence":"additional","affiliation":[{"name":"Welsh School of Pharmacy, Cardiff University, Cardiff, United Kingdom,"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2010,2,4]]},"reference":[{"key":"atypb1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-03-01066.1996"},{"key":"atypb2","doi-asserted-by":"publisher","DOI":"10.1089\/152308603768295249"},{"key":"atypb3","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2007.11.003"},{"key":"atypb4","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.nutr.24.012003.132418"},{"key":"atypb5","author":"Elsey DJ","journal-title":"Cell Biochem 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