{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T06:11:38Z","timestamp":1777615898033,"version":"3.51.4"},"reference-count":20,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2010,10,12]],"date-time":"2010-10-12T00:00:00Z","timestamp":1286841600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Cardiovasc Pharmacol Ther"],"published-print":{"date-parts":[[2011,3]]},"abstract":"<jats:p>Hypoxia-reoxygenation (HR) is a major driver for angiogenesis in atherosclerotic plaques and tumors. Angiogenesis is a multistep process requiring stimulation of proliferation and migration of endothelial cells in response to a number of growth factors, including transforming growth factor (TGF\u03b21). Aspirin (acetylsalicylic acid) has been shown to reduce atherosclerosis-related events as well as development of certain tumors. We examined the role of aspirin in HR-mediated angiogenesis from human umbilical vein endothelial cells (HUVECs). We found that aspirin (0.5 and 1 mmol\/L) markedly (by about 30%, P &lt; .01) reduced HR-mediated tube formation. Next, we studied changes in TGF\u03b21 and its type 1 receptor (TGF\u03b2-R1) as well as in the transcription of p53 and p21 during HR-mediated angiogenesis. Hypoxia-reoxygenation modestly increased TGF\u03b21 and decreased its type 1 receptor (TGF\u03b2-R1) transcription (both P-NS) and reduced the transcription of p53 and p21 (P &lt; .05). Treatment of HUVECs with aspirin suppressed TGF\u03b21 and enhanced TGF\u03b2-R1 mRNA expression during HR (both P &lt; .05 vs HR alone) without a change in p53 and p21 (P-NS). In other experiments, treatment of cells with TGF\u03b21 antibody modestly decreased HR-mediated angiogenesis; however, TGF\u03b21 antibody treatment significantly enhanced the inhibitory effect of aspirin on tube formation. Based on these data, we suggest that the inhibitory effect of aspirin on HR-mediated angiogenesis involves TGF\u03b21-TGF\u03b2-R1 pathway.<\/jats:p>","DOI":"10.1177\/1074248410378505","type":"journal-article","created":{"date-parts":[[2010,10,17]],"date-time":"2010-10-17T22:27:38Z","timestamp":1287354458000},"page":"105-110","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":11,"title":["Angiostatic Effects of Aspirin in Hypoxia-Reoxygenation Are Linked to Modulation of TGF\u03b21 Signaling"],"prefix":"10.1177","volume":"16","author":[{"given":"Magomed","family":"Khaidakov","sequence":"first","affiliation":[{"name":"Division of Cardiovascular Medicine, University of Arkansas for Medical Sciences and VA Medical Center, Little Rock, AR, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacob","family":"Szwedo","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Medicine, University of Arkansas for Medical Sciences and VA Medical Center, Little Rock, AR, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sona","family":"Mitra","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Medicine, University of Arkansas for Medical Sciences and VA Medical Center, Little Rock, AR, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jawahar L.","family":"Mehta","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Medicine, University of Arkansas for Medical Sciences and VA Medical Center, Little Rock, AR, USA, or"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2010,10,12]]},"reference":[{"key":"atypb1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000233387.90257.9b"},{"key":"atypb2","doi-asserted-by":"publisher","DOI":"10.1080\/10611860802088523"},{"key":"atypb3","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2007.10.009"},{"key":"atypb4","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.107.152272"},{"key":"atypb5","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.107.096446"},{"key":"atypb6","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M708820200"},{"key":"atypb7","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200969060-00001"},{"key":"atypb8","doi-asserted-by":"publisher","DOI":"10.5551\/jat.12.185"},{"key":"atypb9","doi-asserted-by":"publisher","DOI":"10.1159\/000200449"},{"key":"atypb10","doi-asserted-by":"publisher","DOI":"10.1096\/fj.06-5987com"},{"key":"atypb11","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1746.2003.03152.x"},{"key":"atypb12","author":"Khaidakov M.","journal-title":"Cardiovasc Res"},{"key":"atypb13","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.107.166405"},{"key":"atypb14","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.4.1603"},{"issue":"2","key":"atypb15","doi-asserted-by":"crossref","first-page":"1837","DOI":"10.1242\/jcs.115.9.1837","volume":"115","author":"van Wetering S.","year":"2002","journal-title":"J Cell Sci"},{"key":"atypb16","doi-asserted-by":"publisher","DOI":"10.2741\/3573"},{"key":"atypb17","doi-asserted-by":"publisher","DOI":"10.1016\/S1050-1738(03)00142-7"},{"key":"atypb18","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M104536200"},{"key":"atypb19","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.048942"},{"key":"atypb20","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-1818"}],"container-title":["Journal of Cardiovascular Pharmacology and Therapeutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1074248410378505","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1074248410378505","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T16:38:47Z","timestamp":1777394327000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1074248410378505"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,10,12]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2011,3]]}},"alternative-id":["10.1177\/1074248410378505"],"URL":"https:\/\/doi.org\/10.1177\/1074248410378505","relation":{},"ISSN":["1074-2484","1940-4034"],"issn-type":[{"value":"1074-2484","type":"print"},{"value":"1940-4034","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,10,12]]}}}