{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T06:28:56Z","timestamp":1777530536530,"version":"3.51.4"},"reference-count":55,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2019,4,29]],"date-time":"2019-04-29T00:00:00Z","timestamp":1556496000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["NS094896"],"award-info":[{"award-number":["NS094896"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Cardiovasc Pharmacol Ther"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Uncontrollable bleeding is a major cause of mortality and morbidity worldwide. Effective hemostatic agents are urgently needed. Red cell microparticles (RMPs) are a highly promising hemostatic agent. This study evaluated the safety profile of RMPs preliminary to clinical trial.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Results:<\/jats:title>\n                    <jats:p>RMPs were prepared from type O+ human red blood cell by high-pressure extrusion. Male rats were treated with RMPs either a 1 \u00d7 bolus, or 4 \u00d7 or 20 \u00d7 administered over 60 minutes. The vehicle-treated group was used as a control. Effects on physiological parameters were evaluated; namely, blood pressure, body and head temperature, hematocrit, and blood gases. We did not observe any adverse effects of RMPs on these physiological parameters. In addition, brain, heart, and lungs of rats treated with 4 \u00d7 dose (bolus followed by infusion over 60 minutes) or vehicle were examined histologically for signs of thrombosis or other indications of toxicity. No thrombosis or indications of toxicity in brain, heart, or lungs were observed. Studies revealed that RMPs were distributed mainly in liver, spleen, and lymph nodes, and were potentially excreted through the kidneys.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Our study indicates that RMP administration appears not to have any negative impact on the parameters studied and did not produce thrombosis in heart, brain, and lungs. However, more detailed long-term studies confirming the safety of RMP as a hemostatic agent are warranted.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1074248419838512","type":"journal-article","created":{"date-parts":[[2019,4,30]],"date-time":"2019-04-30T00:55:11Z","timestamp":1556585711000},"page":"474-483","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Preclinical Evaluation of Safety and Biodistribution of Red Cell Microparticles: A Novel Hemostatic Agent"],"prefix":"10.1177","volume":"24","author":[{"given":"Ashish K.","family":"Rehni","sequence":"first","affiliation":[{"name":"Peritz Scheinberg Cerebral Vascular Disease Research Laboratories, University of Miami Miller School of Medicine, Miami, FL, USA"},{"name":"Department of Neurology, University of Miami Miller School of Medicine, Miami, FL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vibha","family":"Shukla","sequence":"additional","affiliation":[{"name":"Peritz Scheinberg Cerebral Vascular Disease Research Laboratories, University of Miami Miller School of Medicine, Miami, FL, USA"},{"name":"Department of Neurology, University of Miami Miller School of Medicine, Miami, FL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hever","family":"Navarro Quero","sequence":"additional","affiliation":[{"name":"Wallace H Coulter Platelet Laboratory, Division of Hematology and Oncology, Department of Medicine, University of Miami Miller School of Medicine, Miami, FL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"Carlos","family":"Bidot","sequence":"additional","affiliation":[{"name":"Wallace H Coulter Platelet Laboratory, Division of Hematology and Oncology, Department of Medicine, University of Miami Miller School of Medicine, Miami, FL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Conner R.","family":"Haase","sequence":"additional","affiliation":[{"name":"Peritz Scheinberg Cerebral Vascular Disease Research Laboratories, University of Miami Miller School of Medicine, Miami, FL, USA"},{"name":"Department of Neurology, University of Miami Miller School of Medicine, Miami, FL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ensign Anise A.","family":"Crane","sequence":"additional","affiliation":[{"name":"Peritz Scheinberg Cerebral Vascular Disease Research Laboratories, University of Miami Miller School of Medicine, Miami, FL, USA"},{"name":"Department of Neurology, University of Miami Miller School of Medicine, Miami, FL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6776-5206","authenticated-orcid":false,"given":"Shivam G.","family":"Patel","sequence":"additional","affiliation":[{"name":"Peritz Scheinberg Cerebral Vascular Disease Research Laboratories, University of Miami Miller School of Medicine, Miami, FL, USA"},{"name":"Department of Neurology, University of Miami Miller School of Medicine, Miami, FL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastian","family":"Koch","sequence":"additional","affiliation":[{"name":"Peritz Scheinberg Cerebral Vascular Disease Research Laboratories, University of Miami Miller School of Medicine, Miami, FL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yeon S.","family":"Ahn","sequence":"additional","affiliation":[{"name":"Wallace H Coulter Platelet Laboratory, Division of Hematology and Oncology, Department of Medicine, University of Miami Miller School of Medicine, Miami, FL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenche","family":"Jy","sequence":"additional","affiliation":[{"name":"Wallace H Coulter Platelet Laboratory, Division of Hematology and Oncology, Department of Medicine, University of Miami Miller School of Medicine, Miami, FL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0173-5338","authenticated-orcid":false,"given":"Kunjan R.","family":"Dave","sequence":"additional","affiliation":[{"name":"Peritz Scheinberg Cerebral Vascular Disease Research Laboratories, University of Miami Miller School of Medicine, Miami, FL, USA"},{"name":"Department of Neurology, University of Miami Miller School of Medicine, Miami, FL, USA"},{"name":"Neuroscience Program, University of Miami Miller School of Medicine, Miami, FL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2019,4,29]]},"reference":[{"key":"bibr1-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1097\/01.ta.0000199961.02677.19"},{"key":"bibr2-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1097\/00005373-199905000-00003"},{"key":"bibr3-1074248419838512","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2016.160754"},{"key":"bibr4-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0154857"},{"key":"bibr5-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2007.06949.x"},{"issue":"139","key":"bibr6-1074248419838512","first-page":"155","volume":"22","year":"2013","journal-title":"Prescrire Int"},{"key":"bibr7-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.112.675231"},{"key":"bibr8-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1007\/s00268-007-9087-2"},{"key":"bibr9-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2017.0639"},{"key":"bibr10-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1592\/phco.28.4.485"},{"key":"bibr11-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.107.493601"},{"key":"bibr12-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1160\/TH08-08-0521"},{"key":"bibr13-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1615646"},{"issue":"10","key":"bibr14-1074248419838512","doi-asserted-by":"crossref","first-page":"7171","DOI":"10.1016\/S0021-9258(18)53161-6","volume":"268","author":"Chang CP","year":"1993","journal-title":"J Biol Chem"},{"key":"bibr15-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0104200"},{"key":"bibr16-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.110.233403"},{"key":"bibr17-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3148.2008.00892.x"},{"issue":"4","key":"bibr18-1074248419838512","first-page":"751","volume":"110","author":"Jy W","year":"2013","journal-title":"Thromb Haemost"},{"key":"bibr19-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2005.05.004"},{"key":"bibr20-1074248419838512","doi-asserted-by":"publisher","DOI":"10.4103\/0976-500X.81895"},{"key":"bibr21-1074248419838512","first-page":"16022","volume":"3","author":"Huang Y","year":"2016","journal-title":"Molecular Therapy"},{"key":"bibr22-1074248419838512","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.00599"},{"key":"bibr23-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2006.12.015"},{"key":"bibr24-1074248419838512","doi-asserted-by":"publisher","DOI":"10.3109\/09537104.2012.749397"},{"issue":"5","key":"bibr25-1074248419838512","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1111\/j.1537-2995.2007.01630.x-i2","volume":"48","author":"Bosman GJ","year":"2008","journal-title":"Transfusion"},{"key":"bibr26-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1007\/s11239-015-1299-y"},{"key":"bibr27-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-011-0198-4"},{"key":"bibr28-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1038\/srep17611"},{"key":"bibr29-1074248419838512","doi-asserted-by":"crossref","first-page":"3770","DOI":"10.1182\/blood.V128.22.3770.3770","volume":"128","author":"Cho SJW","year":"2016","journal-title":"Blood"},{"key":"bibr30-1074248419838512","doi-asserted-by":"crossref","first-page":"3769","DOI":"10.1182\/blood.V128.22.3769.3769","volume":"128","author":"Azad MJW","year":"2016","journal-title":"Blood"},{"key":"bibr31-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03095.x"},{"key":"bibr32-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1186\/cc8050"},{"key":"bibr33-1074248419838512","volume-title":"Clinical Methods: The History, Physical, and Laboratory Examinations","author":"Trulock EP","year":"1990","edition":"3"},{"key":"bibr34-1074248419838512","volume-title":"Surgical Treatment: Evidence-Based and Problem-Oriented","author":"Kuhn C","year":"2001"},{"key":"bibr35-1074248419838512","volume-title":"Measurements of Cardiac Function. Cardiovascular Physiology","author":"Mohrman DE","year":"2014","edition":"8"},{"key":"bibr36-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000097829.89204.0C"},{"key":"bibr37-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12008"},{"key":"bibr38-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2012.04758.x"},{"key":"bibr39-1074248419838512","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18091850"},{"key":"bibr40-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1172\/JCI26987"},{"key":"bibr41-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2012.10.011"},{"key":"bibr42-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1159\/000442681"},{"key":"bibr43-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1186\/cc13303"},{"key":"bibr44-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1097\/TA.0b013e3182606301"},{"key":"bibr45-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1016\/j.jamcollsurg.2011.12.032"},{"key":"bibr46-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14268"},{"key":"bibr47-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.25376"},{"key":"bibr48-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1038\/srep15636"},{"key":"bibr49-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1002\/jps.24251"},{"key":"bibr51-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2012.05.028"},{"key":"bibr52-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2014.12.013"},{"key":"bibr53-1074248419838512","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1182\/blood.V126.23.1146.1146","volume":"126","author":"Jy WBC","year":"2015","journal-title":"Blood"},{"key":"bibr54-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-07-167148"},{"key":"bibr55-1074248419838512","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-04-1578"},{"key":"bibr56-1074248419838512","volume-title":"Good Laboratory Practice for Nonclinical Laboratory Studies; Proposed Rule Economics Staff, Office of Planning, Office of Policy and Planning, Office of the Commissioner","author":"Food and Drug Administration Department of Health and Human Services","year":"2016"}],"container-title":["Journal of Cardiovascular Pharmacology and Therapeutics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1074248419838512","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1074248419838512","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1074248419838512","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T16:43:00Z","timestamp":1777394580000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.sagepub.com\/doi\/10.1177\/1074248419838512"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,29]]},"references-count":55,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2019,4,29]]},"published-print":{"date-parts":[[2019,9]]}},"alternative-id":["10.1177\/1074248419838512"],"URL":"https:\/\/doi.org\/10.1177\/1074248419838512","relation":{},"ISSN":["1074-2484","1940-4034"],"issn-type":[{"value":"1074-2484","type":"print"},{"value":"1940-4034","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,29]]}}}