{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T16:12:49Z","timestamp":1777479169082,"version":"3.51.4"},"reference-count":14,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Appl Thromb Hemost"],"published-print":{"date-parts":[[2006,1]]},"abstract":"<jats:p>A commercially available turbidometric assay has been evaluated for the measurement of von Willebrand factor ristocetin cofactor activity (VWF:RCo). The assay is simple, rapid, and can be cost-effectively performed on automated coagulation analyzers. This study\u2019s aim is to illustrate the performance of the automated VWF:RCo assay and its capacity to identify patients with von Willebrand disease (VWD). By direct comparison with a conventional VWF:RCo assay, performed on an aggregometer, the concordance between the two assays was 96%. With minor modifications, the automated assay showed a detection level of 0.03 kIU\/L with linearity to 2.00 kIU\/L. The imprecision of the automated assay was reduced compared to the conventional assay procedure with CV of 6.8% at the 1.00 kIU\/L level and 8.6% at the 0.30 kIU\/L level. The automated VWF:RCo assay was also suitable as a screening test to detect VWD in patients investigated for the cause of an increased bleeding tendency. In this situation the automated VWF:RCo assay was tested simultaneously with an automated immunoassay for von Willebrand antigen. Receiver operating curves for the diagnosis of VWD showed a greater area under the curve for the automated VWF:RCo assay compared to the immunoassay, 0.98 vs. 0.94, although the difference did not reach significance. In conclusion, the modified automated VWF:RCo assay shows better precision, lower detection limit, is faster to perform with a lower cost per test compared to the conventional aggregometer based VWF:RCo activity method and is an alternative to an antigen immunoassay as a screening test for VWD.<\/jats:p>","DOI":"10.1177\/107602960601200110","type":"journal-article","created":{"date-parts":[[2006,3,31]],"date-time":"2006-03-31T17:34:07Z","timestamp":1143826447000},"page":"61-67","source":"Crossref","is-referenced-by-count":12,"title":["Evaluation of a Rapid Automated Assay for Analysis of von Willebrand Ristocetin Cofactor Activity"],"prefix":"10.1177","volume":"12","author":[{"given":"Karin","family":"Strandberg","sequence":"first","affiliation":[{"name":"Department of Coagulation Disorders, Department of Clinical Chemistry, Lund University, Malm\u00f6 University Hospital, Malm\u00f6, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Lethagen","sequence":"additional","affiliation":[{"name":"Department of Coagulation Disorders, Lund University, Malm\u00f6 University Hospital, Malm\u00f6, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerstin","family":"Andersson","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry, Lund University, Malm\u00f6 University Hospital, Malm\u00f6, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margareta","family":"Carlson","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry, Lund University, Malm\u00f6 University Hospital, Malm\u00f6, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Hillarp","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry, Lund University, Malm\u00f6 University Hospital, Malm\u00f6, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2006,1,1]]},"reference":[{"key":"atypb1","doi-asserted-by":"publisher","DOI":"10.1053\/beha.2001.0136"},{"key":"atypb2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2516.2002.00611.x"},{"key":"atypb3","doi-asserted-by":"publisher","DOI":"10.1053\/beha.2001.0133"},{"key":"atypb4","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1613224"},{"key":"atypb5","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2001.02597.x"},{"key":"atypb6","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2516.1999.0050s2028.x"},{"key":"atypb7","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1613027"},{"key":"atypb8","first-page":"362","volume":"26","author":"Howard MA","year":"1971","journal-title":"Thromb Diathes Heamorrh"},{"key":"atypb9","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1657727"},{"key":"atypb10","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1613765"},{"key":"atypb11","doi-asserted-by":"publisher","DOI":"10.1054\/blre.1999.0116"},{"key":"atypb12","first-page":"306","volume":"34","author":"Macfarlane DE","year":"1975","journal-title":"Thromb Diath Haemorrh"},{"key":"atypb13","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(78)90268-2"},{"key":"atypb14","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1613154"}],"container-title":["Clinical and Applied Thrombosis\/Hemostasis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/107602960601200110","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/107602960601200110","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T14:05:02Z","timestamp":1777385102000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/107602960601200110"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,1]]},"references-count":14,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2006,1]]}},"alternative-id":["10.1177\/107602960601200110"],"URL":"https:\/\/doi.org\/10.1177\/107602960601200110","relation":{},"ISSN":["1076-0296","1938-2723"],"issn-type":[{"value":"1076-0296","type":"print"},{"value":"1938-2723","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,1]]}}}