{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T16:15:48Z","timestamp":1777479348913,"version":"3.51.4"},"reference-count":33,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Clinica Colsanitas"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Clin Appl Thromb Hemost"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Accurate diagnosis of Von Willebrand Disease (VWD) is essential for effective management, but no single test can definitively confirm it due to the disease's complexity and the limitations of current methods. Tests measuring Von Willebrand Factor (VWF) binding activity, such as the platelet-dependent VWF activity test (VWF:Ab), have been developed, but their application in Latin American populations remains underexplored. This study aimed to evaluate the performance of the VWF:Ab test compared to the Ristocetin Cofactor test (VWF:GPIbR) in a Colombian population with suspected or confirmed VWD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A diagnostic cross-sectional and retrospective study was conducted, including adults referred for hematological evaluation due to a personal or family history of bleeding disorders. Samples were analyzed using both VWF:GPIbR and VWF:Ab tests (HemosIL\n                      <jats:sup>\u00ae<\/jats:sup>\n                      ) simultaneously. Diagnostic performance was assessed through sensitivity, specificity, predictive values. Additionally, correlation analyses were performed, and the level of agreement at various diagnostic thresholds was assessed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>125 patients were included, 88% of whom were women. A strong positive correlation was found between both tests (ICC = 0.96, p\u2009&lt;\u20090.001). VWF:Ab had a specificity of 98.8% and sensitivity of 71.4%. Degree of agreement for 89.6% threshold was Normal (Greater than 50 IU\/dL), Pathological (Less than 50.0 IU\/dL). Notably, 7.2% of samples were classified as unclassifiable by VWF:Ab, highlighting potential limitations in qualitative defect detection<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>VWF:Ab exhibited high specificity and a strong correlation with VWF:GPIbR. Nevertheless, the differences in subtype classification and the identification of unclassifiable samples underscore the necessity of using both tests in a complementary manner.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/10760296251401583","type":"journal-article","created":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T16:05:08Z","timestamp":1766073908000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Diagnostic Performance of Von Willebrand Activity Versus Ristocetin Cofactor Tests in the Primary Evaluation of Von Willebrand Disease in Colombia"],"prefix":"10.1177","volume":"31","author":[{"given":"Marcela","family":"Godoy-Corredor","sequence":"first","affiliation":[{"name":"Clinica Colsanitas, Grupo Keralty"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1847-4282","authenticated-orcid":false,"given":"Claudia C.","family":"Colmenares-Mejia","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Universitaria Sanitas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aura M.","family":"Reina-Soler","sequence":"additional","affiliation":[{"name":"Member of the Von Willebrand Committee Latin American Group of Thrombosis and Hemostasis"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0094-6421","authenticated-orcid":false,"given":"Natalia","family":"Gomez-Lopera","sequence":"additional","affiliation":[{"name":"Clinica Colsanitas, Grupo Keralty"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana Lozano-","family":"Gait\u00e1n","sequence":"additional","affiliation":[{"name":"Clinica Colsanitas, Grupo Keralty"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego A.","family":"Ruiz-Amaya","sequence":"additional","affiliation":[{"name":"Clinica Colsanitas, Grupo Keralty"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ximena","family":"Guti\u00e9rrez-Urue\u00f1a","sequence":"additional","affiliation":[{"name":"Clinica Colsanitas, Grupo Keralty"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario A.","family":"Isaza-Ruget","sequence":"additional","affiliation":[{"name":"Clinica Colsanitas, Grupo Keralty"},{"name":"Fundaci\u00f3n Universitaria Sanitas"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,12,18]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/jth.14591"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.hoc.2021.07.004"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2019.07.010"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1080\/10408363.2021.2014781"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1182\/hematology.2019000064"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2022007216"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-024-00536-8"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.2147\/JBM.S389241"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/hae.14547"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.2147\/IJGM.S321605"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/jth.14145"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/rth2.12807"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12220"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/rth2.12513"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/jth.14304"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0091687"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2020003265"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2021005431"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0025-7753(05)72210-7"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1055\/a-1806-9972"},{"key":"e_1_3_2_22_2","unstructured":"HemosIL. 2020. Von Willebrand Factor Ristocetin cofactor. Instrumentation Laboratory. Retrieved from http:\/\/www.ilww.com."},{"key":"e_1_3_2_23_2","unstructured":"Instrumentation Laboratory Company. HemosIL. (2020). Von Willebrand Factor Activity- 0020004700 Protocol. Instrumentation Laboratory. Retrieved from http:\/\/www.ilww.com."},{"key":"e_1_3_2_24_2","unstructured":"HemosIL. (2020). von Willebrand Factor Antigen \u2013 0020002300. Instrumentation Laboratory. Retrieved from http:\/\/www.ilww.com."},{"key":"e_1_3_2_25_2","unstructured":"HemosIL. 2020. Factor VIII deficient plasma \u2013 0020012800. Instrumentation Laboratory. Retrieved from http:\/\/www.ilww.com."},{"key":"e_1_3_2_26_2","unstructured":"HemosIL. 2017. LAB Tromboplastina Parcial Activada PTT SynthASil \u2013 0020006800. Instrumentation Laboratory. Retrieved from http:\/\/www.ilww.com."},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2014.04.033"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/hae.13957"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rpth.2023.100139"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12345"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12218"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-10-249102"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2016.09.003"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/jth.12594"}],"container-title":["Clinical and Applied Thrombosis\/Hemostasis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10760296251401583","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/10760296251401583","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10760296251401583","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T14:05:30Z","timestamp":1777385130000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/10760296251401583"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8]]},"references-count":33,"alternative-id":["10.1177\/10760296251401583"],"URL":"https:\/\/doi.org\/10.1177\/10760296251401583","relation":{},"ISSN":["1076-0296","1938-2723"],"issn-type":[{"value":"1076-0296","type":"print"},{"value":"1938-2723","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8]]},"article-number":"10760296251401583"}}