{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T16:17:09Z","timestamp":1777479429958,"version":"3.51.4"},"reference-count":17,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Clin Appl Thromb Hemost"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Defibrotide is a DNA-derived polyelectrolyte with anti-ischemic and antithrombotic effects, primarily used in post-bone marrow transplantation (BMT) veno-occlusive disease (VOD). Currently, it is produced from porcine mucosal tissue, which presents cultural, economic, and supply limitations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>This study compared coagulation activity, molecular profiles, and DNA content of porcine- versus ovine-derived defibrotide.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Active pharmaceutical ingredient (API) samples of 19 porcine and 9 ovine defibrotide batches were dissolved in saline (1\u2005mg\/mL) and added to pooled plasma and whole blood (100\u2005\u00b5g\/mL). Coagulation was assessed using activated partial thromboplastin time (aPTT) and thrombin time (TT). Anti-Xa and anti-IIa activities were measured by amidolytic assays. DNA content was quantified via the heparin red method, and molecular weight was determined by size exclusion chromatography.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Neither porcine nor ovine preparations showed anticoagulant effects in aPTT. Both produced mild, comparable anticoagulant activity in TT. Anti-Xa and anti-IIa values were similar across sources. Molecular weight profiles were also comparable (ovine: 16.8\u2009\u00b1\u20090.24\u2005kDa vs porcine: 18.02\u2009\u00b1\u20090.32\u2005kDa). DNA content showed no significant difference (ovine: 75.78 \u03bcg vs porcine: 79 \u03bcg\/10 \u03bcg API).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Ovine- and porcine-derived defibrotide demonstrated equivalent coagulation, molecular, and DNA content profiles. These findings suggest ovine mucosa is a viable alternative source for defibrotide, potentially improving accessibility, reducing cost, and addressing dietary restrictions associated with porcine-derived products.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/10760296261429228","type":"journal-article","created":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T10:10:03Z","timestamp":1774260603000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["A Comparative Study of Porcine and Ovine Derived Defibrotide: Coagulation Profiles, Molecular Characteristics, and DNA Content"],"prefix":"10.1177","volume":"32","author":[{"given":"Hussein","family":"Abourahma","sequence":"first","affiliation":[{"name":"Loyola University, Chicago Cardiovascular Institute"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2901-0339","authenticated-orcid":false,"given":"Prakasha","family":"Kempaiah","sequence":"additional","affiliation":[{"name":"Loyola University, Stritch School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ambar","family":"Farooqui","sequence":"additional","affiliation":[{"name":"Loyola University, Chicago Cardiovascular Institute"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emily","family":"Krupa","sequence":"additional","affiliation":[{"name":"Loyola University, Chicago Cardiovascular Institute"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omer","family":"Iqbal","sequence":"additional","affiliation":[{"name":"Loyola University, Chicago Cardiovascular Institute"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3465-2499","authenticated-orcid":false,"given":"Jawed","family":"Fareed","sequence":"additional","affiliation":[{"name":"Loyola University, Chicago Cardiovascular Institute"},{"name":"Loyola University, Stritch School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiming","family":"Yao","sequence":"additional","affiliation":[{"name":"Loyola University, Stritch School of Medicine"},{"name":"Ronnsi Pharmaceuticals, Jiangsu Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2026,3,23]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2022.103372"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2009.08.024"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2021005410"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/mtna.2016.42"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2013.05.001"},{"key":"e_1_3_4_7_2","volume-title":"LiverTox: Clinical and Research Information on Drug-Induced Liver Injury","author":"Defibrotide","year":"2012","unstructured":"Defibrotide. LiverTox: Clinical and Research Information on Drug-Induced Liver Injury. National Institute of Diabetes and Digestive and Kidney Diseases; 2012. Accessed August 7, 2025. http:\/\/www.ncbi.nlm.nih.gov\/books\/NBK548496\/"},{"issue":"1","key":"e_1_3_4_8_2","first-page":"21","article-title":"Effect of defibrotide on platelet function","volume":"22","author":"Ulutin ON","year":"1996","unstructured":"Ulutin ON, Balkuv-Ulutin S, Bezer-G\u00f6ker B, et al. Effect of defibrotide on platelet function. Semin Thromb Hemost. 1996;22(Suppl 1):21\u201324.","journal-title":"Semin Thromb Hemost"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-199345020-00007"},{"key":"e_1_3_4_10_2","unstructured":"World Population Clock: 8.2 Billion People (LIVE 2025) - Worldometer. 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