{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T08:14:30Z","timestamp":1788077670018,"version":"build-2784847793"},"reference-count":31,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"2025 University-Level Basic Medical Research Project Training Camp Special Program","award":["2025QN105"],"award-info":[{"award-number":["2025QN105"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Clin Appl Thromb Hemost"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Sepsis is frequently complicated by systemic inflammatory injury and cardiac dysfunction, which serve as risk factors of adverse clinical outcomes and increased mortality risk. To clarify the function of miR-130a-3p in sepsis diagnosis and its regulatory role in sepsis-induced inflammatory injury and cardiac dysfunction, based on clinical analysis and cellular assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The study recruited 126 patients with sepsis and an matched number of healthy controls. The potential of miR-130a-3p to differentiate between sepsis and healthy controls was evaluated by receiver operating characteristic (ROC) curve. Quantify miR-130a-3p and PDK4 levels using real-time quantitative polymerase chain reaction (RT-qPCR). Indicators of inflammation, cardiac dysfunction and oxidative stress in cells were assessed by corresponding biochemical assays and enzyme-linked immunosorbent assay (ELISA) kits. The functional properties of H9C2 cells were evaluated by cell counting kit-8 (CCK-8) assay and flow cytometry. Bioinformatics analysis and luciferase activity assays were employed to identify the downstream targets of miR-130a-3p.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>H9C2 cell viability was impaired, with the increased levels of interleukin-1beta (IL-1\u03b2), interleukin-6 (IL-6), cardiac troponin I (cTnI), creatine kinase MB (CK-MB), malondialdehyde (MDA), and decreased superoxide dismutase (SOD) activity. When miR-130a-3p expression was repressed, the levels of inflammation and cardiac dysfunction indicators were diminished, and the oxidative stress damage and cell viability was restored. PDK4 is a direct target of miR-130a-3p and is low-expressed in sepsis patients and LPS-induced H9C2 cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>miR-130a-3p modulates inflammatory response and cardiac dysfunction by mediating PDK4, representing a potential effective target for sepsis diagnosis and intervention.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/10760296261468449","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T13:03:06Z","timestamp":1787835786000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["miR-130a-3p Exacerbates Sepsis-Induced Cardiac Dysfunction by Promoting Inflammation and Targeting PDK4"],"prefix":"10.1177","volume":"32","author":[{"given":"Jinyu","family":"Liu","sequence":"first","affiliation":[{"name":"Affiliated Rudong Hospital of Xinglin College"},{"name":"Nantong University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Yuan","sequence":"additional","affiliation":[{"name":"Second Military Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2489-9586","authenticated-orcid":false,"given":"Wei","family":"Chen","sequence":"additional","affiliation":[{"name":"The First People\u2019s Hospital of Lanzhou"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongqin","family":"Shen","sequence":"additional","affiliation":[{"name":"Jiangsu Provincial Hospital of Chinese Medicine Chongqing Hospital (Chong-qing Yongchuan District Traditional Chinese Medicine 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